In Vitro Production of Embryo and Cryopreservation as the Assistant Technology of Producing Quality Beef Cattle

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Chatree Chumnandee
Nawarat Pha-obnga
Malai Chongcharoen


The objective of this study was to observe the techniques of in vitro embryo production and cryopreservation at the laboratory level as the assistant technology of producing quality beef cattle. A total of 124 embryos with at least 4 cells which developed from 342 cumulus oocyte complexes (COCs) were divided into 3 groups; group 1; non-vitrification (control), group 2; microdroplets vitrification  (MDV), and group 3; solid surface vitrification (SSV). The results showed that the proportion of fertilization oocytes was 52.9% (181/342). The proportion of embryos with at least 4 cells was 36.3% (124/342). The vitrified embryos in MDV and SSV groups can not to develop into morula and blastocyst stage. The proportion of blastocyst (4.9%; 2/40) in control group was not different among groups (P>0.05), while proportion of morula (26.8%; 11/40) was significantly higher than those of both vitrified groups (P≤0.05). The embryo quality in MDV group was classified at score 3 (10.0%) and score 4 (50.0%), as well as in SSV group was classified at score 3 (2.3%) and score 4 (39.5%) which not different between vitrified groups (P>0.05). In conclusion, the results showed that in vitro embryo production in our laboratory can produce embryos at 4-16 cells and a few of blastocysts. However, the vitrified embryos cannot develop into other stages.


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