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The objective of this study was to observe the techniques of in vitro embryo production and cryopreservation at the laboratory level as the assistant technology of producing quality beef cattle. A total of 124 embryos with at least 4 cells which developed from 342 cumulus oocyte complexes (COCs) were divided into 3 groups; group 1; non-vitrification (control), group 2; microdroplets vitrification (MDV), and group 3; solid surface vitrification (SSV). The results showed that the proportion of fertilization oocytes was 52.9% (181/342). The proportion of embryos with at least 4 cells was 36.3% (124/342). The vitrified embryos in MDV and SSV groups can not to develop into morula and blastocyst stage. The proportion of blastocyst (4.9%; 2/40) in control group was not different among groups (P>0.05), while proportion of morula (26.8%; 11/40) was significantly higher than those of both vitrified groups (P≤0.05). The embryo quality in MDV group was classified at score 3 (10.0%) and score 4 (50.0%), as well as in SSV group was classified at score 3 (2.3%) and score 4 (39.5%) which not different between vitrified groups (P>0.05). In conclusion, the results showed that in vitro embryo production in our laboratory can produce embryos at 4-16 cells and a few of blastocysts. However, the vitrified embryos cannot develop into other stages.
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