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Leptospirosis is an important zoonotic disease, especially for farmers and those involved in animal husbandry. Infected swamp buffaloes usually are asymptomatic but infertility issues may present themselves. This study was designed to detect pathogenic Leptospira spp. by polymerase chain reaction (PCR) technique in commercially available and widely used frozen semen samples from 56 swamp buffalo bulls in Thailand. DNA samples were extracted and grouped into seven experimental group samples (eight samples per test tube). Polymerase Chain Reaction assays were performed using single primer LipL32 and 16S rRNA and multiplex primer Ph In and Sa. The results showed that Leptospira spp. was not detected in any of the seven experimental group samples. However, penicillin and streptomycin were added in the extenders of these frozen semen samples, which could have acted as antibiotics for Leptospira spp. or The buffalo bulls were healthy without Leptospira spp. Infection.
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