The On Top Supplementation of Endoxylanase Enhances Growth Performance and Alleviates Oxidative Stress in Broiler Fed Diets Containing High Non-Starch Polysaccharide
Main Article Content
Abstract
This study was conducted to evaluate the effects of dietary on-top xylanase supplementation on growth performance and oxidative stress of broilers fed diets containing high non-starch polysaccharide (NSP). A total of 1,500 one-day-old male Ross 308 broiler chicks were randomly assigned to 5 treatments with 12 replicates per treatment (25 birds per replicate) using completely randomized design (CRD). The basal diet was formulated to contain high level of wheat and barley as sources of NSP. The experimental diets were supplemented with 5 different levels of xylanase including 0, 1,200, 2,400, 4,800, and 9,600 U/g feed, respectively. The experiment was run for a period of 35 days. During the 1-10 and 11-24 days of age (DOA), dietary xylanase supplementation improved body weight gain (BWG, P<0.05) and feed conversion ratio (FCR, P<0.05) of broilers, but had no effect on feed intake when compared to that of the control group. No significant difference in growth performance among dietary treatments was observed during 25-35 DOA. For overall performance (1-35 DOA), dietary xylanase supplementation had no effect on feed intake. However, xylanase supplementation increased BWG (P<0.05) and improved FCR (P<0.01) of broilers when compared to that of the control group. The on-top supplementation of xylanase at ³ 2,400 U/g feed significantly decreased serum MDA of broilers (P<0.01). The results showed that the on-top supplementation xylanase supplementation improved growth performance and reduced oxidative stress of broilers fed high NSP diets.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Anwar, U., Riaz, M., Farooq Khalid, M., Mustafa, R., Farooq, U., Ashraf, M., Munir, H., Auon, M., Hussain, M. and Hussain, M. 2023. Impact of exogenous xylanase and phytase, individually or in combination, on performance, digesta viscosity and carcass characteristics in broiler birds fed wheat-based diets. Animals. 13(2), 278.
Arczewska-Wlosek, A., Swiatkiewicz, S., Bederska-Lojewska, D., Orczewska-Dudek, S., Szczurek, W., Boros, D., Fras, A., Tomaszewska, E., Dobrowolski, P. and Muszynski, S. 2019. The efficiency of xylanase in broiler chickens fed with increasing dietary levels of rye. Animals. 9(2): 46.
Aviagen. 2019. Ross broiler: Nutrition specifications. The local Aviagen, US.
Bedford, M.R. and Cowieson, A.J. 2012. Exogenous enzymes and their effects on intestinal microbiology. Anim. Feed Sci. Technol. 173(1-2): 76-85.
Cardoso, V., Fernandes, E., Santos, H., Maçãs, B., Lordelo, M., Telo da Gama, L., Ferreira, L., Fontes, C. and Ribeiro, T. 2018. Variation in levels of non-starch polysaccharides and endogenous endo-1, 4-β-xylanases affects the nutritive value of wheat for poultry. Br. Poult. Sci. 59(2): 218-226.
Choct, M. and Annison, G. 1992. Anti-nutritive effect of wheat pentosans in broiler chickens: Roles of viscosity and gut microflora. Br. Poult. Sci. 33(4): 821-834.
Choct, M., Hughes, R.J., Wang, J., Bedford, M., Morgan, A. and Annison, G. 1996. Increased small intestinal fermentation is partly responsible for the anti‐nutritive activity of non‐starch polysaccharides in chickens. Br. Poult. Sci. 37(3): 609-621.
Claeson Bohnstedt, K. 2005. Determination of biomarkers for lipid peroxidation and oxidative stress: Development of analytical techniques and methods, PhD. Thesis. Institutionen för analytisk kemi.
Cleophas, M. C., Crişan, T.O., Lemmers, H., Toenhake-Dijkstra, H., Fossati, G., Jansen, T.L., Dinarello, C.A., Netea, M.G. and Joosten, L.A. 2016. Suppression of monosodium urate crystal-induced cytokine production by butyrate is mediated by the inhibition of class I histone deacetylases. Ann. Rheum. Dis. 75(3): 593-600.
Dai, D., Wu, S.-g., Zhang, H.-j., Qi, G.-h. and Wang, J. 2020. Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model. J. Anim. Sci. Biotechnol. 11: 1-16.
Damen, B., Pollet, A., Dornez, E., Broekaert, W.F., Haesendonck, I.V., Trogh, I., Arnaut, F., Delcour, J.A. and Courtin, C.M. 2012. Xylanase-mediated in situ production of arabinoxylan oligosaccharides with prebiotic potential in whole meal breads and breads enriched with arabinoxylan rich materials. Food Chem. 131(1): 111-118.
DeKeyser, K., Dierick, N., Kuterna, L., Maigret, O., Kaczmarek, S., Rutkowski, A. and Vanderbeke, E. 2018. Non-starch polysaccharide degrading enzymes in corn and wheat-based broiler diets: dual activity for major substrates. J. Agric. Sci. Technol. A. 8(2): 76-88.
Dornez, E., Gebruers, K., Delcour, J.A. and Courtin, C.M. 2009. Grain-associated xylanases: occurrence, variability, and implications for cereal processing. Trends Food Sci. Technol. 20(11-12): 495-510.
Duarte, M. E., Zhou, F.X. Dutra Jr, W.M. and Kim, S.W. 2019. Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs. Anim. Nutr. 5(4): 351-358.
Geissmann, T. and Neukom, H. 1973. note of ferulic acid as a constituent of the water-insoluble pentosans of wheat flour. Cereal Chem. 5: 414-416.
Gonzalez-Ortiz, G., Sola-Oriol, D., Martinez-Mora, M., Perez, J. and Bedford, M.R. 2017. Response of broiler chickens fed wheat-based diets to xylanase supplementation. Poult. Sci. 96(8): 2776-2785.
Gorenz, B., Iseri, V., Rubach, J. and Dilger, R.N. 2022. Xylanase supplementation of pelleted wheat-based diets increases growth efficiency and apparent metabolizable energy and decreases viscosity of intestinal contents in broilers. Poult. Sci. 101(12): 102220.
Grotto, D., Santa Maria, L., Boeira, S., Valentini, J., Charão, M., Moro, A., Nascimento, P., Pomblum, V. and Garcia. S. 2007. Rapid quantification of malondialdehyde in plasma by high performance liquid chromatography–visible detection. J. Pharm. Biomed. Anal. 43(2): 619-624.
Iji, P., Saki, A. and Tivey, D. 2001. Body and intestinal growth of broiler chicks on a commercial starter diet. 1. Intestinal weight and mucosal development. Br. Poult. Sci. 42(4): 505-513.
Inayah, S. R., Mutia, R., Jayanegara, A., Yanza, Y.R. and Amnah, S. 2022. Effects of xylanase supplementation on the performance, nutrient digestibility, and digestive organ profiles of broiler chickens: a meta-analysis. World. Poult. Res. 12: 199-211.
Jha, R. and Mishra, P. 2021. Dietary fiber in poultry nutrition and their effects on nutrient utilization, performance, gut health, and on the environment: a review. J. Anim. Sci. Biotechnol. 12: 1-16.
Kiarie, E., Walsh, M., Romero, L., Arent, S. and Ravindran, V. 2017. Nutrient and fiber utilization responses of supplemental xylanase in broiler chickens fed wheat based diets are independent of the adaptation period to test diets. Poult. Sci. 96(9): 3239-3245.
Knudsen, K.E.B. 2014. Fiber and nonstarch polysaccharide content and variation in common crops used in broiler diets. Poult. Sci. 93(9): 2380-2393.
Koh, A., De Vadder, F., Kovatcheva-Datchary, P. and Bäckhed, F. 2016. From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites. Cell. 165(6): 1332-1345.
Kouzounis, D., Jonathan, M.C., Soares, N., Kabel, M.A. and Schols, H.A. 2022. In vivo formation of arabinoxylo-oligosaccharides by dietary endo-xylanase alters arabinoxylan utilization in broilers. Carb. Poly. 291: 119527.
Lee, S., Apajalahti, J., Vienola, K., González-Ortiz, G., Fontes, C.M.G.A. and Bedford, M.R. 2017. Age and dietary xylanase supplementation affects ileal sugar residues and short chain fatty acid concentration in the ileum and caecum of broiler chickens. Anim. Feed Sci. Technol. 234: 29-42.
Lee, S. H., Hosseindoust, A., Laxman Ingale, S., Rathi, P.C., Yoon, S.Y., Choi, J.W. and Kim, J.S. 2020. Thermostable xylanase derived from Trichoderma citrinoviride increases growth performance and non-starch polysaccharide degradation in broiler chickens. Br. Poult. Sci. 61(1): 57-62.
Lei, Z., Shao, Y., Yin, X., Yin, D., Guo, Y. and Yuan, J. 2016. Combination of xylanase and debranching enzymes specific to wheat arabinoxylan improve the growth performance and gut health of broilers. J. Agric. Food Chem. 64(24): 4932-4942.
Lin, Q., Ou, S. and Wen, Q. 2014. In vitro antioxidant activity of feruloyl arabinose isolated from maize bran by acid hydrolysis. J. Food Sci. Technol. 51: 1356-1362.
Liu, D., Guo, S. and Guo, Y. 2012. Xylanase supplementation to a wheat-based diet alleviated the intestinal mucosal barrier impairment of broiler chickens challenged by Clostridium perfringens. Avian Pathol. 41(3): 291-298.
Liu, W.-C. and Kim, I.-H. 2017. Effects of dietary xylanase supplementation on performance and functional digestive parameters in broilers fed wheat-based diets. Poult. Sci. 96(3): 566-573.
Mahmoud, A. M., Hussein, O.E., Hozayen, W.G., Bin-Jumah, M. and Abd El-Twab, S.M. 2020. Ferulic acid prevents oxidative stress, inflammation, and liver injury via upregulation of Nrf2/HO-1 signaling in methotrexate-induced rats. Environ. Sci. Pollut. Res. 27: 7910-7921.
Masey-O’Neill, H., Singh, M. and Cowieson, A. 2014. Effects of exogenous xylanase on performance, nutrient digestibility, volatile fatty acid production and digestive tract thermal profiles of broilers fed on wheat-or maize-based diet. Br. Poult. Sci. 55(3): 351-359.
McGee, D., Bamberg, T., Vitkus, S. and McGhee, J. 1995. A synergistic relationship between TNF-alpha, IL-1 beta, and TGF-beta 1 on IL-6 secretion by the IEC-6 intestinal epithelial cell line. J. Immunol. 86(1): 6.
Mishra, B. and Jha, R. 2019. Oxidative stress in the poultry gut: Potential challenges and interventions. Front. Vet. Sci. 6: 60.
Moita, V. H. C., Duarte, M.E. and Kim. S.W. 2022. Functional roles of xylanase enhancing intestinal health and growth performance of nursery pigs by reducing the digesta viscosity and modulating the mucosa-associated microbiota in the jejunum. J. Anim. Sci. 100(5): skac116.
Montagne, L., Crévieu-Gabriel, I., Toullec, R. and Lallès, J.P. 2003. Influence of dietary protein level and source on the course of protein digestion along the small intestine of the veal calf. J. Dairy Sci. 86(3): 934-943.
Motta, F., Andrade, C. and Santana, M. 2013. A review of xylanase production by the fermentation of xylan: classification, characterization and applications. Sustainable degradation of lignocellulosic biomass-techniques, applications commercialization. 251-276 p.
Niazi, A., Khan, A., Alina, Z., Asghar, A., Ishfaq, B., Kousar, S., Riaz, L., Abbas, R. and Naseer, F. 2017. Effect of supplementation of xylanase on feed efficiency and serum biochemistry in broilers. Res. j. Pharmacol. Pharmacy. 1(1): 1-9.
Passos, A. A., Park, I., Ferket, P., von Heimendahl, E. and Kim, S.W. 2015. Effect of dietary supplementation of xylanase on apparent ileal digestibility of nutrients, viscosity of digesta, and intestinal morphology of growing pigs fed corn and soybean meal based diet. Anim. Nutri. 1(1): 19-23.
Pereira, G. V., Abdel-Hamid, A.M. Dutta, S., D’Alessandro-Gabazza, C.N., Wefers, D., Farris, J.A., Bajaj, S., Wawrzak, Z., Atomi, H. and Mackie, R.I. 2021. Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nat. Commun. 12(1): 459.
Phaniendra, A., Jestadi, D.B. and Periyasamy, L. 2015. Free radicals: properties, sources, targets, and their implication in various diseases. Indian J. Clin. Biochem. 30: 11-26.
Raza, A., Bashir, S. and Tabassum, R. 2019. An update on carbohydrases: growth performance and intestinal health of poultry. Heliyon. 5(4).
Röhe, I. and Zentek, J. 2021. Lignocellulose as an insoluble fiber source in poultry nutrition: a review. J. Anim. Sci. Biotechnol. 12(1): 82.
Rosicka-Kaczmarek, J., Komisarczyk, A., Nebesny, E. and Makowski, B. 2016. The influence of arabinoxylans on the quality of grain industry products. European Food Research and Technology. 242: 295-303.
Ruemmele, F., J. Beaulieu, S. Dionne, E. Levy, E. Seidman, N. Cerf-Bensussan, and M. Lentze. 2002. Lipopolysaccharide modulation of normal enterocyte turnover by toll-like receptors is mediated by endogenously produced tumour necrosis factor α. Gut. 51(6): 842-848.
Saeton, W., Jitviriyanon, S., Songserm, O., and Ruangpanit, Y. 2021. Effect of NSPase supplementation in wheat containing diet on performance, digesta viscosity and apparent metabolizable energy of broiler. J. Mahanakorn Vet. Med. Vol.16 No.1: 53-62. (in Thai)
SAS. 2021. SAS® On Demand for academics: Studio. SAS Institute Inc. Cary, North Carolina, USA.
Singh, Z., Karthigesu, I.P., Singh, P. and K. Rupinder, K. 2014. Use of malondialdehyde as a biomarker for assessing oxidative stress in different disease pathologies: a review. Iran. J. Public Health. 43(supple 3): 7-16.
Smeets, N., Nuyens, F., Van Campenhout, L., Delezie, E. and Niewold, T. 2018. Interactions between the concentration of non-starch polysaccharides in wheat and the addition of an enzyme mixture in a broiler digestibility and performance trial. Poult. Sci. 97(6): 2064-2070.
Tiwari, U. P., Chen, H. Kim, S.W. and Jha, R. 2018. Supplemental effect of xylanase and mannanase on nutrient digestibility and gut health of nursery pigs studied using both in vivo and in vitro models. Anim. Feed Sci. Technol. 245: 77-90.
Usami, M., Kishimoto, K., Ohata, A., Miyoshi, M., Aoyama, M., Fueda, Y. and Kotani, J. 2008. Butyrate and trichostatin A attenuate nuclear factor κB activation and tumor necrosis factor α secretion and increase prostaglandin E2 secretion in human peripheral blood mononuclear cells. Nutr. Res. 28(5): 321-328.
Van Hoeck, V., Papadopoulos, G.A., Giannenas, I., Lioliopoulou, S., Tsiouris, V., Mantzios, T., Kiskinis, K., Grivas, I., Gonzalez Sanchez, A.L., Vasanthakumari, B.L., Fortomaris, P. and Morisset, D. 2021. New intrinsically thermostable xylanase improves broilers’ growth performance, organ weights, and affects intestinal viscosity and pH. Agriculture. 11(12): 1235.
Vasanthakumari, B., Gedye, K., Abdollahi, M., Di Benedetto, M., Sanchez, D. G., Wealleans, A. and Ravindran, V. 2023. A new monocomponent xylanase improves performance, ileal digestibility of energy and nutrients, intestinal morphology, and intestinal microbiota in young broilers. J. Appl. Poult. Res. 32(1), 100301.
Wang, J., Liu, S., Ma, J. and Piao, X. 2021. Changes in growth performance and ileal microbiota composition by xylanase supplementation in broilers fed wheat-based diets. Front. Microbiol. 12: 706396.
Yang, P., Hu, H., Li, Y., Ai, Q., Zhang, W., Zhang, Y. and Mai, K. 2019. Effect of dietary xylan on immune response, tight junction protein expression and bacterial community in the intestine of juvenile turbot (Scophthalmus maximus L.). Aquaculture. 512: 734361.
Zhang, L., Xu, J., Lei, L., Jiang, Y., Gao, F. and Zhou, G. 2014. Effects of xylanase supplementation on growth performance, nutrient digestibility and non-starch polysaccharide degradation in different sections of the gastrointestinal tract of broilers fed wheat-based diets. Asian-Aust. J. Anim. Sci. 27(6): 855.
Zhang, S., Wang, C., Sun, Y., Wang, G., Chen, H., Li, D., Yu, X. and Chen, G. 2018. Xylanase and fermented Polysaccharide of Hericium caputmedusae reduce pathogenic infection of broilers by improving antioxidant and anti-inflammatory properties. Oxid. Med. Cell. Longev. 2018(1): 4296985.
Zhang, Z., Yang, P. and Zhao, J. 2022. Ferulic acid mediates prebiotic responses of cereal-derived arabinoxylans on host health. Anim. Nutri. 9: 31-38.