การทดสอบความรุนแรงของเชื้อ Vibrio parahaemolyticus ในปูทะเล (Scylla sp.)
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ก.ค. 20, 2017
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ความรุนแรงของเชื้อ ปูทะเล วิบริโอ พาราฮีโมไลติคัส
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บทคัดย่อ
การศึกษาการก่อให้เกิดโรคในปูทะเล (Scylla sp.) ที่ได้รับเชื้อ Vibrio parahaemolyticus โดยการนำปูทะเลสุขภาพดีมาทำการฉีดเชื้อ V. parahaemolyticus ที่ปริมาณความเข้มข้น 101-1010 CFU (Colony Forming Unit) ต่อมิลลิลิตรหลังจากฉีดเชื้อแบคทีเรียที่ปริมาณความเข้มข้น 108-1010 พบว่าปูมีอัตราการตายสะสม 100 เปอร์เซ็นต์ภายในเวลา 1 วัน และความเข้มข้นเชื้อแบคทีเรีย 107 CFU ต่อมิลลิลิตร ทำให้ปูมีอัตราการตายสะสม 100 เปอร์เซ็นต์ ภายใน 2 วัน ส่วนที่ความเข้มข้นเชื้อปริมาณ 106 CFU ต่อมิลลิลิตร ทำให้ปูมีอัตราการตายสะสม 22.22 เปอร์เซ็นต์ ภายใน 30 วัน นอกจากนี้ที่ปริมาณความเข้มข้น 101-105 CFU ต่อมิลลิลิตร พบว่าปูไม่มีอัตราการตายสะสมเกิดขึ้นซึ่งการก่อโรคของ V. parahaemolyticus ทำให้เกิดความรุนแรงถึงขั้นทำให้ปูทะเลตายได้อย่างรวดเร็ว
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รูปแบบการอ้างอิง
Srimeetian, P., Suwanmala, J. และ Tunkijjanukij, S. (2017) “การทดสอบความรุนแรงของเชื้อ Vibrio parahaemolyticus ในปูทะเล (Scylla sp.)”, สัตวแพทย์มหานครสาร, 12(1), น. 19–27. available at: https://li01.tci-thaijo.org/index.php/jmvm/article/view/114186 (สืบค้น: 21 ธันวาคม 2025).
ประเภทบทความ
บทความวิจัย
เอกสารอ้างอิง
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Ayres, P.A. and Edwards, E. 1982. Notes on the distribution of black spot shell disease in crustacean fisheries. Chem. Ecol. 1: 125-130.
Chaweepack, T., Ruangpan, L. and Nuphin, V. 2007. Parasites and vibrio flora of wild broodstock banana shrimp (Penaeus merguiensis) from eastern gulf of Thailand. Technical paper. Coastal Fisheries Research and Development Bureau. Chanthaburi Coastal Fisheries Research and Development Center; Department of Fisheries, Chanthaburi (Thailand). (in Thai)
Costa-Ramos, C. and Rowley, A. F. 2004. Effect of extracellular products of Pseudoalteromonas atlantica on the edible crab Cancer pagurus. Appl. Environ. Microbiol. 70(2): 729-735.
Davis, W. J. and Sizemore, K. R.1982. Incidence of Vibrio species associated with Blue Crabs Callinectes sapidus collected from Galveston Bay, Texas. Appl. Environ. Microbiol. 43(5): 1092-1097.
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Ruangpan, L., Tanasomwang, W. and Sangrungruang, K. 1997. Bacterial flora of intensively cultured black tiger shrimp (Penaeus monodon). Proceedings of the 35th Kasetsart University Annual Conference: Fisheries, Science, Engineering, Environmental Management, Home Economics, Education and Economics. (in Thai)
Somjetlertcharoen, A. 1993. Study on Vibrio species and oxolinic acid residues from Penaeus monodon Fabricius in intensive culture ponds. The thesis of Kasetsart University. Bangkok. (in Thai)
Srimeetian, P. 2010. Antioxidant and lipid peroxidation in molting cycle of mud crab (Scylla serrata Forskål 1775). The thesis of Kasetsart University. Bangkok. (in Thai)
Srimeetian, P., and Tunkijjanukij, S. 2016. Differential protein expression in haemolymph of first-stage red sternum mud crabs (Scylla serrata). Thai J. Vet. Med. 46(3): 401-407.
Sudheesh, P.S. and Xu, H.S. 2001. Pathogenicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon Fabricius: possible role of extracellular proteases. Aquaculture.196: 37-46.
Vogan, C.L. and Rowley, A.F. 2002. Effects of shell disease syndrome on the haemocytes and humoral defences of the edible crab, Cancer pagurus. Aquaculture. 205: 237–252.
Aguirre-Guzmán, G., Sánchez-Martínez, G. J., Perez-Castañeda, R., Palacios-Monzón, A., Trujillo-Rodríguez, T. and Cruz-Hernández, I. N. 2010. Pathogenicity and infection route of Vibrio parahaemolyticus in American White Shrimp, Litopenaeus vannamei. J. World Aquacult. Soc. 41(3): 464-470.
Ayres, P.A. and Edwards, E. 1982. Notes on the distribution of black spot shell disease in crustacean fisheries. Chem. Ecol. 1: 125-130.
Chaweepack, T., Ruangpan, L. and Nuphin, V. 2007. Parasites and vibrio flora of wild broodstock banana shrimp (Penaeus merguiensis) from eastern gulf of Thailand. Technical paper. Coastal Fisheries Research and Development Bureau. Chanthaburi Coastal Fisheries Research and Development Center; Department of Fisheries, Chanthaburi (Thailand). (in Thai)
Costa-Ramos, C. and Rowley, A. F. 2004. Effect of extracellular products of Pseudoalteromonas atlantica on the edible crab Cancer pagurus. Appl. Environ. Microbiol. 70(2): 729-735.
Davis, W. J. and Sizemore, K. R.1982. Incidence of Vibrio species associated with Blue Crabs Callinectes sapidus collected from Galveston Bay, Texas. Appl. Environ. Microbiol. 43(5): 1092-1097.
Direkbusarakom, S. and Rojanapitayakul, S. 1998. Effect of chlorine (Calcium hypochlorite) in seawater and seawater with soil on Vibrio harveyi. Proceedings of the 36th Kasetsart University Annual Conference: Abstracts. (in Thai)
Intanakom, J. 2010. Study on morphological, bacterial identification and histology in mud crab (Scylla sp.) with red thoracic-abdominal syndrome. The thesis of Kasetsart University. Bangkok. (in Thai)
Laoprasert, T. and Laoprasert, S. 2010. Diseases in mud crab, Scylla sp. Thai Fisheries Gazette (Thailand). 63: 265-267. (in Thai)
Laoprasert, T., Laoprasert, S., Soonthornwit, S. and Phonwari, N. 2010. Study on bacterial flora in mud crab (Scylla sp.). Thai Fisheries Gazette (Thailand). 63: 238-241. (in Thai)
Plainpun, N. 2014. Induction of red thoracic – abominal syndrome with bacteria isolated from mud crab (Scylla sp.). The thesis of Kasetsart University. Bangkok. (in Thai)
Promchairat., J., Limsuwan, C. and Chuchird, N. 2013. Experimental exposure and evaluation of Artemia sp. to Vibrio sp. As possible vectors of pathogenic bacteria. Proceedings of 51st Kasetsart University Annual Conference: Veterinary Medicine, Fisheries. (in Thai)
Ruangpan, L., Tanasomwang, W. and Sangrungruang, K. 1997. Bacterial flora of intensively cultured black tiger shrimp (Penaeus monodon). Proceedings of the 35th Kasetsart University Annual Conference: Fisheries, Science, Engineering, Environmental Management, Home Economics, Education and Economics. (in Thai)
Somjetlertcharoen, A. 1993. Study on Vibrio species and oxolinic acid residues from Penaeus monodon Fabricius in intensive culture ponds. The thesis of Kasetsart University. Bangkok. (in Thai)
Srimeetian, P. 2010. Antioxidant and lipid peroxidation in molting cycle of mud crab (Scylla serrata Forskål 1775). The thesis of Kasetsart University. Bangkok. (in Thai)
Srimeetian, P., and Tunkijjanukij, S. 2016. Differential protein expression in haemolymph of first-stage red sternum mud crabs (Scylla serrata). Thai J. Vet. Med. 46(3): 401-407.
Sudheesh, P.S. and Xu, H.S. 2001. Pathogenicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon Fabricius: possible role of extracellular proteases. Aquaculture.196: 37-46.
Vogan, C.L. and Rowley, A.F. 2002. Effects of shell disease syndrome on the haemocytes and humoral defences of the edible crab, Cancer pagurus. Aquaculture. 205: 237–252.
