Genetic relationship among Paphiopedilum parishii ( Rchb. f. ) Stein, from two sources of Northern provinces using amplified fragment length polymorphism (AFLP) technique

Authors

  • กนกอร ศรีม่วง International Professional Training and Service in Agriculture and Food, School of Agriculture and Natural Resources, University of Phayao, Phayao Province 56000
  • ศุภลักษณ์ ภูมิคง The Botanical Garden Organization, Ministry of Natural Resources and Environment, Mae Rim District, Chiangmai Province 50180

Keywords:

Orchidaceae, Paphiopedilum parishii (Rchb.f.) Stein, genetic relationship

Abstract

The lady slipper, Paphiopedilum parishii (Rchb.f.) Stein (lady slipper), is named based on their habitat such as Kanchanaburi, and Chiang Dao. For P. parishii (Chiang Dao) district is also namely P. dianthum Tang & Wang. The authors aimed to investigate the genetic relationship of lady slippers among the samples from 2 sources, using amplified fragment length polymorphism (AFLP) technique. The results revealed relatively low genetic differentiation (66.11%).

References

1. Academic Segment, Resear and Development Center. Paphiopedilum parishii (Rchb.f.) The Botanical Garden Organization ( BGO) , Ministry of Natural Resource and Environmental, Thailand. Available from: 31https://www.qsbg.org/database/botanic_book%20full%20option/search_detail.asp?botanic_id=1350

2. Surin Peyachoknagul. DNA markers: from basic to application. Bangkok: Kasetsart University; 2009.

3. Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990;12(1):13-15.

4. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, et al. AFLP: A new technique for DNA fingerprintings. Nucleic Acid Res. 1995; 23:4407-4414.

5. Rolf FJ. NTSYSpc: Numerical taxonomy and multivariate analysis system version 2.1. User Guide. New York: Exeter Setauket; 2000

6. Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979;76(10):5269-5273.

7. Nei M. Genetic distance between populations. Am Nat. 1972; 106:283-292.

8. Sneath PHA, Sokal RR, Numerical Taxonomy: The principles and practice of numerical classification. San Francisco: Freeman WH and Company; 1973.

9. Yeh FC, Yang RC, Boyle T. Popgene. Microsoft Window- based freeware for population genetic analysis. Release 1. 31. Canada; University of Alberta; 1999.

10. Wanichkul J, Changtragoon S. Investigation of genetic diversity of Paphiopedilum exul by using AFLP (amplified fragment length polymorphism) markers. Proceeding of biodiversity Research from the past to the future; 2005 Aug 21-24; Phetchaburi, Thailand.

11. Frankham R, Ballou JD, Briscoe DA. Introduction to conservation genetics. Cambridge: Cambridge University Press; 2002. 617 p.12. Li A, Luo YB, Xiong ZT, Ge S. A preliminary study on conservation genetics of three endangered orchids species. Acta Botanica Sinica. 2002;44(2):250-252.

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Published

2019-08-30

How to Cite

1.
ศรีม่วง ก, ภูมิคง ศ. Genetic relationship among Paphiopedilum parishii ( Rchb. f. ) Stein, from two sources of Northern provinces using amplified fragment length polymorphism (AFLP) technique. Health Sci Tech Rev [Internet]. 2019 Aug. 30 [cited 2024 Nov. 17];12(2):41-5. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/200403

Issue

Vol. 12 No. 2 (2019): May - August

Section

Research articles

License

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