Selection of free-living nitrogen fixing bacteria to promote on rice growth

  • จตุพร บุณณดากุล Department of Biotechnology, Faculty of Science, Maejo University, Chiangmai Province 50290
  • ศิราภรณ์ ชื่นบาล Department of Environmental Technology, Faculty of Science, Maejo University, Chiangmai Province 50290
  • ฐปน ชื่นบาล Department of Environmental Technology, Faculty of Science, Maejo University, Chiangmai Province 50290
  • ศรีกาญจนา คล้ายเรือง Department of Biotechnology, Faculty of Science, Maejo University, Chiangmai Province 50290
  • ศุภธิดา อํ่าทอง Department of Soil Science, Faculty of Agricultural Production, Maejo University, Chiangmai Province 50290
Keywords: Nitrogen fixing bacteria, rice rhizosphere soil, bio-fertilizer


The study was aimed to isolate the bacteria from rice rhizosphere soil samples, and selected the freeliving nitrogen fixing bacteria. There were totally 310 isolates with 26 isolates for nitrogen fixing bacteria. The qualification testing showed 10 bacterial isolates, with the high effectiveness of nitrogen fixation and indole-3- acetic acid (IAA) production. Identification of selected bacteria using analyzing the 16S rRNA genes sequence indicated three species such as Bacillus altitudinis CM2-2, Bacillus aryabhattai LP1-5 and Pseudomonas nitroreducens PY7-6, which had produced high nitrogen compounds at 15.60 ± 0.40, 7.70 ± 0.70 and 6.30 ± 0.10 mg/L respectively. Beside, Pseudomonas nitroreducens PY7-6 had provided the highest amount of IAA at 23.90 ± 0.20 mg/L. The NPK chemical fertilizer and isolated bacteria had been applied into the rice pot during the 30-day seedling period. The NPK and Bacillus altitudinis CM2-2 was the most treatment for the rice growth including the stem height, root length, leaf number, and fresh and dry weight, significant differences compared with the control. However, there was no significant difference of the nitrogen contents. In conclusion, Bacillus altitudinis CM2-2 might be developed as bio-fertilizer.


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How to Cite
บุณณดากุลจ, ชื่นบาลศ, ชื่นบาลฐ, คล้ายเรืองศ, อํ่าทองศ. Selection of free-living nitrogen fixing bacteria to promote on rice growth. Naresaun Phayao J [Internet]. 2019Aug.30 [cited 2020Jul.10];12(2):32-0. Available from:
Research articles