In vitro culture of Cleisostoma arietinum for conservation: Race orchid species

Authors

  • สุมนา เหลืองฐิติกาญจนา Department of Biotechnology, School of Agriculture and Natural Resources, University of Phayao, Phayao Province 56000
  • กนกอร ศรีม่วง International Professional Training and Service in Agriculture and Food, School of Agriculture and Natural Resources, University of Phayao, Phayao Province 56000
  • วาสนา พิทักษ์พล Department of Agriculture, School of Agriculture and Natural Resources, University of Phayao, Phayao Province 56000

Keywords:

Cleisostoma arietinum, tissue culture, in vitro

Abstract

The study is aimed to perform tissue culture of the seed of Cleisostoma arietinum (Rchb.f.) Garay for propagation on MS (Murashige and Skoog) medium or VW (Vacine and Went) medium without/with the various concentration of BA (6-benzylaminopurine). The result showed that the shoots were induced by VW medium supplemented with coconut water, and BA or kinetin, while the roots were convinced by the media with coconut water, and NAA (1-napthyl acetic acid) or IBA [4-(indol-3-yl) butyric acid] at various concentrations. The plantlets were transplanted into the pots, and the survival rate was 57.4% after 4 weeks.

References

1. Cleisotoma arietinum (Rchb.f.) Garay. Available from: https://www.orchidcambodia.com/cleisosto ma-arietinum.html

2. Distabanjong K, Distabanjong C, Ruengwiset B. In vitro propagation and conservation of Rhynchostylis gigantea (Lindl.) Ridl.). Bangkok: Proceedings of 48th Kasetsart University Annual Conference; 2010:571-578.

3. Panis B, Lambardi M. Status of cryopreser- vation technologies in plants (crops and forest trees). Proceedings of the Role of Biotechnology. Italy: Villa Gualino;2005:43-54.

4. Hirano T, Godo T, Mii M, Ishikawa K. Cryopre-servation of immature seeds of Bletilla striata by vitrification. Plant Cell Rep. 2005;23:534-539.

5. Maneerattanarungroj P, Bunnag S, Monthatong M. In vitro conservation of Cleisostoma areitinum (Rchb.f.) garay, rare Thai orchid species by an encapsulation-dehydration method. Asian J Plant Sci. 2007;6:1235-1240.

6. Sungkumlong, Deb CR. Effects of different factors on immature embryo culture, PLBs differentiation and rapid mass multiplication of Coelogyne suaveolens (Lindl.) Hook. Indian J Exp Biol. 2008;46:243-248.

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Published

2019-04-24

How to Cite

1.
เหลืองฐิติกาญจนา ส, ศรีม่วง ก, พิทักษ์พล ว. In vitro culture of Cleisostoma arietinum for conservation: Race orchid species. Health Sci Tech Rev [Internet]. 2019 Apr. 24 [cited 2024 Apr. 19];12(1):13-6. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/202882

Issue

Vol. 12 No. 1 (2019): January - April

Section

Research articles

License

ผู้นิพนธ์ต้องรับผิดชอบข้อความในบทนิพนธ์ของตน มหาวิทยาลัยพะเยาไม่จำเป็นต้องเห็นด้วยกับบทความที่ตีพิมพ์เสมอไป ผู้สนใจสามารถคัดลอก และนำไปใช้ได้ แต่จะต้องขออนุมัติเจ้าของ และได้รับการอนุมัติเป็นลายลักษณ์อักษรก่อน พร้อมกับมีการอ้างอิงและกล่าวคำขอบคุณให้ถูกต้องด้วย

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