In vitro growth of Dioscorea alata L. by different concentrations of mannitol
Keywords:
Slowing growth, mannitol, Dioscorea alata L.Abstract
The study was aimed to determine in vitro growth of Dioscorea alata L. by different concentrations of mannitol for the optimum medium on slowing growth for conservation of D. alata L. in vitro. Nodal segments were cultured on solid MS (Murashige and skoog, 1962) with the addition of mannitol at different concentrations of 4 levels: 0, 1, 2 and 3 % (w/v) of culture at a temperature of 25 ± 2 °C light for 16 hours a day, the light intensity of 2,500 lux cultured for 90 days without any subculture. Number of shoots, nodes and leaves per plant, percentage of root formation, and shoot length were recorded. The results investigated that 2% (w/v) mannitol was obtained the lowest of number of shoots per plant, percentage of root formation, and shoot length. While, 3% (w/v) of mannitol gave the lowest of number of nodes and leaves, respectively.
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