In vitro growth of Dioscorea alata L. by different concentrations of mannitol

  • Nootjaree Singphan คณะเทคโนโลยีการเกษตรและเทคโนโลยีอุตสาหกรรม มหาวิทยาลัยราชภัฏเพชรบูรณ์
  • Sureepon Yoddee
Keywords: Slowing growth, mannitol, Dioscorea alata L.

Abstract

The study was aimed to determine in vitro growth of Dioscorea alata L. by different concentrations of mannitol for the optimum medium on slowing growth for conservation of D. alata L. in vitro. Nodal segments were cultured on solid MS (Murashige and skoog, 1962) with the addition of mannitol at different concentrations of 4 levels: 0, 1, 2 and 3 % (w/v) of culture at a temperature of 25 ± 2 °C light for 16 hours a day, the light intensity of 2,500 lux cultured for 90 days without any subculture. Number of shoots, nodes and leaves per plant, percentage of root formation, and shoot length were recorded. The results investigated that 2% (w/v) mannitol was obtained the lowest of number of shoots per plant, percentage of root formation, and shoot length. While, 3% (w/v) of mannitol gave the lowest of number of nodes and leaves, respectively.

Downloads

Download data is not yet available.

References

1. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum. 1962;15(3):473-497.
2. Khalid AK, Weiming C. The effects of mannitol and salinity stresses on growth and biochemical accumulations in lemon balm. Acta Ecol Sinica. 2011; 31(2):112-120.
3. Mahmoud A H M, Taghreed A I. Enhanced in vitro production of Ruta graveolens L. coumarins and rutin by mannitol and ventilation. Plant Cell Tissue Organ Cult. 2012;111(3):335-343.
4. Sabah M H, Kadhim M I, Shatha I Y. Effect of shock and elevated levels of mannitol on callus growth, regeneration and proline accumulation in Ruta graveolens cultures. Int J Curr Microbiol App Sci. 2014; 3(11):479-488.
5. Matin G, Danial K, Gholamreza B. Effect of mannitol on some morphological characteristics of in vitro stevia rebaudiana Bertoni. Biharean Biol. 2017;11(2):94-97.
6. Lersrutaiyotin R, Roongruengchanchai N, Chindonnirat J, Burikam S, Wongmanirot M. Growth of sugarcane plantlets on media limiting concentration of inorganic nutrient and growth promoter supplement. Kasetsart J Nat Sci. 1993;27:4-8.
7. Golmirzaie A, Toledo J. (1999). Noncryogenic, long-term germplasm storage. In:Hall RD. editor. Methods in Molecular Biology, Vol. 111: Plant cell culture protocols. Totowa: Humana Press Inc; p. 95-101.
8. Helena L, Dick V. Uptake of mannitol from the media by in vitro grown plants. Plant Cell Tissue Organ Cult. 1996;45(2):103-107.
Published
2020-04-26
How to Cite
1.
Singphan N, Yoddee S. In vitro growth of Dioscorea alata L. by different concentrations of mannitol. Naresaun Phayao J [Internet]. 2020Apr.26 [cited 2020Aug.8];13(1):21-5. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/221381
Section
Research articles