Cytotoxicity against breast cancer cell line of 7R-acetylmelodorinol isolated from Xylopia pierrei Hance

  • Chutamas Thepmalee Division of Biochemistry School of Medical Sciences University of Phayao
  • Natchalida Deesa
  • Ananda Thongsri
  • Ratchanaporn Chokchaisiri
  • Lucksagoon Ganranoo
  • Chonthida Thephinlap
  • Krissana Khoothiam
  • Nittiya Suwannasom
  • Aussara Panya
Keywords: 7R-acetylmelodorinol, Breast cancer cell line, Cytotoxicity activity

Abstract

7R-acetylmelodorinol is extracted and purified compound from stem bark of Xylopia pierrei Hance. Previous studies, it was reported that 7R-acetylmelodorinol has the cytotoxicity activity against human small cell lung cancer (NCI-H187). However, the cytotoxicity activity against breast cancer is still unclear. The aim of the study is to determine cytotoxicity effect of 7R-acetylmelodorinol in breast cancer cell lines. The cell viabilities of MCF-7 breast cancer cell line after treated with various concentrations of 7R-acetylmelodorinol were evaluated by crystal violet staining, MTT assay, and clonogenic assay, respectively. For the crystal violet staining, treatment cells with 50 and 100 µM of 7R-acetylmelodorinol significantly decreased cell viability of breast cancer cells for 24 and 48 hours. The IC50 of 7R-acetylmelodorinol for 24 and 48 hours in MCF-7 was 8.57±0.012 and 7.80±0.013 µM, respectively. The result of clonogenic assay revealed that colony formation showed a significant decrease in breast cancer cell treated with 7R-acetylmelodorinol at concentrations of 4 and 8 µM (p< 0.01). Taken together, 7R-acetylmelodorinol has cytotoxicity activity against breast cancer cells by three different methods. These findings suggested that 7R-acetylmelodorinol might be a useful compound for breast cancer therapy.

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Published
2021-04-30
How to Cite
1.
Thepmalee C, Deesa N, Thongsri A, Chokchaisiri R, Ganranoo L, Thephinlap C, Khoothiam K, Suwannasom N, Panya A. Cytotoxicity against breast cancer cell line of 7R-acetylmelodorinol isolated from Xylopia pierrei Hance. Naresaun Phayao J [Internet]. 2021Apr.30 [cited 2021Sep.23];14(1):25-2. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/247035
Section
Research articles