Genetic diversity, Phytochemical composition and bioactivity, of saba snake grass, (Clinacanthus nutans (Burm. f.) Lindau) of Northern Thailand

Authors

  • Salinee Chiangchin Faculty of Science, Chiang Mai University
  • Supawadee Thongyim Faculty of Science, Chiang Mai University
  • Hataichanok Pundith Faculty of Science, Chiang Mai University
  • Thida Kaewkod Faculty of Science, Chiang Mai University
  • Angkhana Inta Faculty of Science, Chiang Mai University
  • Yingmanee Tragoolpua Faculty of Science, Chiang Mai University
  • Santi Watthana Institute of Science, Suranaree University of Technology
  • Siriphorn Rotarayanont Faculty of Science, Chiang Mai University
  • Aussara Panya Faculty of Science, Chiang Mai University

Keywords:

Sabah snake grass, Crude extract, Phytochemical composition, Bioactivity

Abstract

The study aimed to investigate genetic diversity, phytochemical composition, and bioactivity of Clinacanthus nutans collected from tree provinces of northern Thailand including Chiang Mai, Chiang Rai, and Phayao using sequence-related amplified polymorphism (SRAP-PCR) technique. The data suggested that C. nutans could be clearly differentiated from those of the same family. Thin layer chromatography was performed to determine the major phytochemical composition in C. nutans extract. The results showed that the major composition in C. nutans extract was flavonoid. We investigated the bioactivity of the extracts. The results showed that the extracts of C. nutans taken from Chiang Mai possessed the highest antioxidant and antibacterial activity. A significant correlation was found between the antioxidant and antibacterial activity of extract and the highest amount of flavonoid content.

References

Kunsorn P, Ruangrungsi N, Lipipun V, Khanboon A, Rungsihirunrat K. The identities and anti-herpes simplex virus activity of Clinacanthus nutans and Clinacanthus siamensis. Asian Pac J Trop Biomed. 2013;3(4): 284-290.

Yoke KY, Jun JT, Soek ST, Siau HM, Gwendoline CLE, Hoe SC, Zuraini A. Clinacanthus nutans Extracts are Antioxidant with Antiproliferative Effect on Cultured Human Cancer Cell Lines. J.Evid.-Based Integr. Med. 2013.

Nik MA, Nik AR, Nurliyana MY, Natasha Nur Afiqah MNF, Mohd AO, Muhajir H, Mohd AL. Antitumor and antioxidant effects of Clinacanthus nutans Lindau in 4 T1 tumorbearing mice. BMC Compl Alternative Med. 2019.

Yang HS, Peng TW, Madhavan P, Abdul MS, Akowuah GA. Phytochemical analysis and antibacterial activity of methanolic extract of Clinacanthus nutans leaf. Int J Drug Dev Res. 2013;5(3): 349-355.

Sangeetha A, Prabu R, Naadeirmuthu and Clara Carol K. In Vitro Screening of Cytotoxic, Antimicrobial and Antioxidant Activities of Clinacanthus nutans (Acanthaceae) leaf extracts. Trop J Pharm Res. 2014;13(9): 1455-1461.

Sekar M, Rashid NA. Formulation, evaluation and antibacterial properties of herbal ointment containing methanolic extract of Clinacanthus nutans leaves. Int J. Pharm Clin Res. 2016;8(8): 1170-1174.

Guo XD, Ma YJ, Parry J, Gao JM, Yu LL, Wang M. Phenolics content and antioxidant activity of tartary buckwheat from different locations. Molecules;16(12): 9850-9867.

Li G, Quiros CF. Sequencerelated amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet. 2001;103: 455-461.

Panya A, Pundith H, Thongyim S, Kaewkod TH, Chitov TH, Bovonsombut S, et al. Antibiotic-Antiapoptotic dual function of Clinacanthus nutans (Burm. f.) Lindau leaf extracts against bovine nastitis. Antibiotics. 2020;9(7): 1-13.

Abramoff MD, Magalhaes PJ, Ram SJ. Image Processing with ImageJ. Biophotonics International. 2004;11(7): 36-42.

Fong SY, Piva T, Urban S, Huynh T. Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae). J Med Plant Res. 2014;8(25): 903-914.

Kennedy DO, Wightman EL. Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain function. Adv Nutr. 2011;2(1): 32-50.

Khoo LW, Kow SA, Lee MT, Tan CP, Shaari K, Tham CL, Abas F. A Comprehensive Review on Phytochemistry and Pharmacological Activities of Clinacanthus nutans (Burm.f.) Lindau. Evid Based Complement Alternat Med. 2018;1-39.

Norliza AL, Chua LS, Siti Nor AA, Rashid, Noor AZ. Antioxidants, Polyphenols and Marker Phytochemicals Content from Different Parts of Clinacanthus nutans (C.nutans). Tran on Sci and Tech. 2017; 4(3-3): 336 - 341.

Swee-Hua EL, Maitha AA, Shaima IA, Swee-Yee C, Aish A, Chun-Wai M, Kok-Song L. Antibacterial Activity of Clinacanthus nutans Polar and Non-Polar Leaves and Stem Extracts. Biomed Pharmacol J 2020;13(3): 1169-1174.

Published

2021-12-01

How to Cite

1.
Chiangchin S, Thongyim S, Pundith H, Kaewkod T, Inta A, Tragoolpua Y, Watthana S, Rotarayanont S, Panya A. Genetic diversity, Phytochemical composition and bioactivity, of saba snake grass, (Clinacanthus nutans (Burm. f.) Lindau) of Northern Thailand. Health Sci Tech Rev [Internet]. 2021 Dec. 1 [cited 2024 Mar. 29];14(3):63-7. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/247147

Issue

Section

Research articles