Insoluble phosphate solubilisation and acid phosphatase activity of bacteria isolated from organic paddy soils

Authors

  • Kittima Waithaisong Microbiology Department Faculty of Science Silpakorn University
  • Kamonrat Kliangpradith Environmental Science Department, Faculty of Science, Silpakorn University, Nakhon Pathom Campus
  • Punnaray Punnasiri Environmental Science Department, Faculty of Science, Silpakorn University
  • Praewpran Thaingthaum Environmental Science Department, Faculty of Science, Silpakorn University
  • Taksawan Thongaram Microbiology Department, Faculty of Science, Silpakorn University
  • Urarux Romruen Microbiology Department, Faculty of Science, Silpakorn University
  • Orawan Borirak Microbiology Department, Faculty of Science, Silpakorn University
  • Porntip Sridang Environmental Science Department, Faculty of Science, Silpakorn University

Keywords:

Organic paddy soils, Phosphate-solubilising bacteria, Tricalcium phosphate solubilisation, Organic phosphorus mineralisation, Acid phosphatase activity

Abstract

An increase in phosphorus (P) availability through the addition of microbial inoculant, such as phosphate-solubilising bacteria (PSB), in organic agricultural soils, could be a good option for increasing soil fertility. We evaluated phosphate-solubilising (PS) ability and phosphate-mineralising (PM) ability of PSB isolated from organic paddy soils. Total soil bacteria were isolated on LB agar and tested on NBRIP agar for tricalcium phosphate (TCP) solubilisation. Three PSB strains were selected, based on their colonial morphologies on LB agar, for the measurement of PS ability in NBRIP broth, and PM ability in the presence of acid phosphatase substrate, p-nitrophenyl phosphate in culture broth. PSB were identified by morphology and a molecular technique using 16S rRNA gene. The results indicated that the selected PSB were able to solubilise TCP and presented acid phosphatase activity. The TCP solubilisation was performed via acid production in NBRIP broth. The strain Ca-106, which was closely related to Bacillus sp. based on the identity of 16S rRNA gene sequences, presented the TCP solubilisation of 181.57 mg P l-1 which was higher than that in the previous report. The other strains (Ca-203 and Ca-270) solubilised TCP with the values of 195.48 mg P l-1 and 180.46 mg P l-1 respectively. They were closely related to Enterobacter sp., based on the identity of 16S rRNA gene sequences. The activity of extracellular acid phosphatase presented the values of 8.12 µM pNP ml-1 h-1, 7.41 µM pNP ml-1 h-1 and 4.40 µM pNP ml-1 h-1 for the strains Ca-106, Ca-203 and Ca-270, respectively. The high activity of acid phosphatase was observed for the selected PSB when compared to the previous reports that used the same technique. Taken together, the results indicated that all selected PSB had both PS ability via the acidification process and PM ability via the production of extracellular acid phosphatase. They can be potentially applied as microbial inoculants for increasing, especially, organic P mineralisation in organic agricultural soils. Further, the PSB strains merit a further study with a target plant, in pot experiments, in order to verify their ability to promote plant growth.

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Bacterial growth curve in NBRIP broth supplemented with 1g l-1 TCP at different time intervals. Mean values (n=3) were plotted against times. Black triangle represented the strain Ca-106, black circle represented the strain Ca-203 and black square represented the strain Ca-270 (A). Graph bars show mean values of in vitro phosphatase activity (n=3) of the phosphatase fraction collected at 24h. Different letters indicated the differences of mean values (ANOVA, p<0.05) (B).

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Published

2022-08-23

How to Cite

1.
Waithaisong K, Kliangpradith K, Punnasiri P, Thaingthaum P, Thongaram T, Romruen U, Borirak O, Sridang P. Insoluble phosphate solubilisation and acid phosphatase activity of bacteria isolated from organic paddy soils. Health Sci Tech Rev [Internet]. 2022 Aug. 23 [cited 2024 Dec. 21];15(2):20-9. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/251284

Issue

Vol. 15 No. 2 (2022): May - September

Section

Research articles

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