Effect of Tinospora crispa (L.) extracts on anti-pyretic activity, platelet amount and blood biochemistry in wistar rats
DOI:
https://doi.org/10.60045/hsstr-2023-253113Keywords:
Boraphet, Fever, Platelet, Blood biochemistryAbstract
Boraphet (Tinospora crispa (L.) Miers ex Hook. f. & Thomson) is a plant found in central Asia and Southeast Asia. Traditional medicines in many countries including Thailand have used parts of the vine, roots and leaves of boraphet for alleviating and treating many diseases such as anti-inflammatory, anti-pyretic, anti-diabetics, immunomodulatory, anti-malarial, anti-bacterial, anticancer as well as for appetizer, etc. This research aimed to evaluate the anti-pyretic effect, the effect on the platelet amount and the blood clinical chemistry of the boraphet extract in Wistar rats. Meanwhile, the ethanolic and aqueous extract of boraphet on these effects were compared. The blood samples were collected at 72 hours prior to the experiment then the fever was induced by diphtheria-tetanus-pertussis (DTP) vaccine. When the fever was detected, the boraphet extract was administered by oral garvage at the doses equivalent to dried boraphet powder of 30, 90 and 270 mg/kg. The body temperatures were measured rectally every 30 minutes until 240 minutes. The blood samples were then collected once again. The results of this study showed that the mean reduction in body temperature of ethanolic extract (TCE) at 30, 90, 270 mg/kg were 1.62±0.33, 1.68±0.51 and 1.48±0.32°C, respectively where as those of aqueous extracts (TCW) were 1.18±0.19, 1.14±0.31 and 1.32±0.36°C, respectively. The positive control groups receiving paracetamol and ibuprofen had the mean temperature reduction of 1.62±0.78 and 1.48±0.18°C, respectively, while the negative control group showed the mean temperature reduction of 0.96±0.31°C. At the end of the experiment, the platelet amount was not affected by the boraphet extracts. Blood biochemistry analyses showed all group values do not difference significantly. However, the rats treated with 270 mg/kg of boraphet aqueous extract had slightly increase in enzyme AST and ALT values, but they were not significantly different from the control groups. It can be concluded that the boraphet ethanolic extracts were more effective in reducing body temperature than aqueous extracts but not significantly different from the positive control groups. The platelet amount and blood biochemistry were not affected by the oral single doses of boraphet extracts significantly. (p>0.05).
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