Antibacterial activity of Murdannia loriformis (Hassk.) on Staphylococcus aureus isolated from the surface of instruments used in kindergarten classrooms
Keywords:
Antibacterial activity, Kindergarten classrooms, Murdannia loriformis (Hassk.)Abstract
This study aimed to examine the antibacterial activity of Murdannia loriformis (Hassk.) leaf ethanol extract. In this study, 72 samples from the surface of instruments used in kindergarten classrooms, such as student desks, wooden toys, and plastic toys, were collected and isolated as types of bacteria, i.e., S. aureus, S. epidermidis, and E. coli. All samples were contaminated with S. aureus (70.8%) and S. epidermidis (62.5%). However, E. coli was not contaminated by the samples. The results of antibacterial activities from M. loriformis leaf ethanol extract at concentrations of 10, 20, and 30 mg/mL using the agar well diffusion method revealed that all concentrations of M. loriformis leaf ethanol extract could inhibit S. aureus with a mean diameter of inhibitory effects at 4.5, 15.17, and 17.50 mm, respectively. Moreover, the result showed that M. loriformis leaf ethanol extract against S. aureus had a minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). MIC values are 6.25 mg/mL, and MBC values are 100 mg/mL.
Keywords: Murdannia loriformis (Hassk.), Staphylococcus aureus, Antibacterial activity, Kindergarten classrooms
References
Wertheim HF, Melles DC, Vos MC, van Leeuwen W, van Belkum A, Verbrugh HA, et al. The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis. 2005;5(12):751-62.
Lin J, Zhang T, Bai C, Liang J, Ye J, Yao Z. School environmental contamination of methicillin-sensitive Staphylococcus aureus as an independent risk factor for nasal colonization in schoolchildren: An observational, cross-sectional study. PLoS One. 2018;3(11):e0208183.
Fritz SA, Krauss MJ, Epplin EK, Burnham CA, Garbutt J, Dunne WM, et al. The natural history of contemporary Staphylococcus aureus nasal colonization in community children. Pediatr Infect Dis J. 2011;30(4):349-51.
Biranjia-Hurdoyal S, Quirin T. Comparative contamination rate of toys in kindergartens and households. Am J Infect Control. 2012;40(6):577-81.
Kaplan SL, Hulten KG, Gonzalez BE, Hammerman WA, Lamberth L, Versalovic J, et al. Three-year surveillance of community-acquired Staphylococcus aureus infections in children. Clin Infect Dis. 2005;40(12):1785-91.
Ledwaba SE, Becker P, Traore-Hoffman A, Potgieter N. Bacterial contamination of children’s toys in rural day care centres and households in South Africa. Int J Environ Res Public Health. 2019,16(2900) https://doi.org/10.3390/ijerph16162900.
El-Kased RF, Gamaleldin NM. Prevalence of bacteria in primary Schools. J Pure Appl Microbiol. 2020;14(4): 2627-36.
Poonthananiwatkul B, Lim RH, Howard RL, Pibanpaknitee P, Williamson EM. Traditional medicine use by cancer patients in Thailand. J Ethnopharmacol. 2015;168:100-7.
Neamsuvan O, Madeebing N, Mah L, Lateh W. A survey of medicinal plants for diabetes treating from Chana and Nathawee district, Songkhla province, Thailand. J Ethnopharmacol. 2015;174:82-90.
Jiratchariyakul W, Kummalue T. Experimental therapeutics in breast cancer cells. Current and Alternative Therapeutic Modalities, IntechOpen, London, UK, 2011.
Jiratchariyakul W, Vongsakul M, Sunthornsuk L, Moongkarndi P, Narintorn A, Somanabandhu A, et al. Immunomodulatory effect and quantitation of a cytotoxic glycosphingolipid from Murdannia loriformis. J Nat Med. 2006;60(3):210-6.
Chomnawang MT, Surassmo S, Nukoolkarn VS, Gritsanapan W. Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria. J Ethnopharmacol. 2005;101(1-3):330-3.
Jiratchariyakul W, Moongkarndi P, Okabe, H, Frahm, A. W. Investigation of anticancer components from Murdannia loriformis (hassk.) Rolla Rao et Kammathy. Thai J Pharmacol. 1998;5(1):10-20.
Vitsutthi M. Antagonistic effect of Staphylococci of extracts from some local plants in Nakorn-Ratchasima province. KKU Sci. J. 2017;45(4):805-16.
Bloemendaal AL, Brouwer EC, Fluit AC. Methicillin resistance transfer from Staphylocccus epidermidis to methicillin-susceptible Staphylococcus aureus in a patient during antibiotic therapy. PLoS One. 2010;5(7); e11841.
U.S. Food and Drug Administration. Bacteriological Analytical Manual Chapter 4: Enumeration of Escherichia coli and the Coliform Bacteria. 2020. Retrieved Sep 5, 2021, from https://www.fda.gov/food/laboratory-methods-food/bam-chapter-4-enumeration-escherichia-coli-and-coliform-bacteria.
CLSI. Performance standards for antimicrobial disk susceptibility tests; Approved standard. 11th ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2012. CLSI document M02-A11.
Burakorn J, Praphruet R. Antibacterial activities of seven indigenous vegetables. J Thai Trad Alt Med. 2012;10(1):11-22.
Kamonwannasit S, Nantapong N, Kumkrai P, Luecha P, Kupittayanant S, Chudapongse N. Antibacterial activity of Aquilaria crassna leaf extract against Staphylococcus epidermidis by disruption of cell wall. Ann. Clin. Microbiol. Antimicrob. 2013;12(20):1-7.
Limsuwan S, Subhadhirasakul S, Voravuthikuncha SP. Medicinal plants with significant activity against important pathogenic bacteria. Pharm Biol. 2009;47(8):683-9.
Ali S, Al-Harbi MM, Rahman SR. Bacterial isolates, present on surface of toys in child care centers, in Al-Rass City, Al-Qassim reigon. K.S.A. EJPMR. 2018;5(5):409-14.
Boretti VS, Corrêa RN, dos Santos SS, Leão MV, Gonçalves e Silva CR. Sensitivity profile of Staphylococcus spp. and Streptococcus spp. isolated from toys used in a teaching hospital playroom. 2014;32(3):151-56.
Aleksejeva V, Dovbenko A, Kroica J, Skadin I. Toys in the playrooms of children's hospitals: A potential source of nosocomial bacterial infections. Children (Basel). 2021;8(10):914.
Avila-Aguero ML, German G, Paris MM, Herrera JF, Safe Toys Study Group. Toys in a pediatric hospital: are they a bacterial source?. Am J Infect Control. 2004;32(5):287-90.
Kasorn N, Nathapindhu G. Type and quantity of microorganisms on the surface of instruments used in kindergarten classrooms of Khon Kaen municipality. KKU Res J (GS). 2010;10(4):97-106.
Che Sulaiman IS, Mohamad A, Ahmed OH. Murdannia loriformis: A Review of Ethnomedicinal Uses, Phytochemistry, Pharmacology, Contemporary Application, and Toxicology. Evid Based Complement Alternat Med. 2021;2021:9976202.
Miklasinska-Majdanik M, Kepa M, Wojtyczka RD, Idzik D, Wasik TJ. Phenolic compounds diminish antibiotic resistance of Staphylococcus Aureus Clinical Strains. Int J Environ Res Public Health. 2018;15(10):2321.
Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005;26(5):343-56.
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