Anticancer potential of Moringa oleifera aqueous leaf extract on human cholangiocarcinoma cells

Authors

  • Jiraporn Jindapol1 Faculty of Medicine, Khon Kaen University
  • Bongkorchporn Bunlear Faculty of Medicine, Khon Kaen University
  • Paramet Poldongnok Faculty of Medicine, Khon Kaen University
  • Panipak Siripaopradit Faculty of Medicine, Khon Kaen University
  • Siriwat Shiraarnon Faculty of Medicine, Khon Kaen University
  • Krittaya Phukmee Faculty of Medicine, Khon Kaen University
  • Laddawan Sengkunpai Faculty of Medicine, Khon Kaen University

Keywords:

Cholangiocarcinoma, Anticancer, Moringa oleifera leaf extract, Apoptosis, Metastasis

Abstract

Cholangiocarcinoma (CCA) is an aggressive malignancy with poor responses to conventional chemotherapeutic agents. At present, the development of new strategies for treatment of this deadly disease is highly desired. Medicinal herbs and dietary plants provide very attractive approaches in chemotherapy. The aim of this study was to determine the potential anticancer effect of Moringa oleifera aqueous leaf extract (MOE) against human CCA cells, KKU-100. According to the MTT assay, MOE decreased CCA cell viability with the IC50 values of 3.8 ± 0.1 and 2.6 ± 0.3 mg/mL at 24 and 48 h, respectively. MOE also inhibited the colony forming capability of the cells as determined by clonogenic assay. Moreover, MOE at 5 mg/mL could induce the apoptotic cell death of CCA cells by which the percentage of early apoptotic cells and late apoptotic/necrosis cells were 10.5 ± 0.7% and 23.7 ± 2.3%, respectively. The anti-migration and anti-invasive effects of the extract were subsequently investigated by wound healing and invasion assay, respectively. The results showed that MOE could significantly suppress migratory and invasive abilities of CCA cells. Taken together, MOE exhibits potential anticancer activities against CCA by limiting ability of cell to thrive, inducing apoptotic cell death and suppressing metastatic ability of CCA cells.  

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รูปแสดงการบุกรุกของเซลล์มะเร็งท่อน้ำดีเมื่อได้รับสารสกัดใบมะรุม 2 mg/mL เทียบกับเซลล์กลุ่มควบคุม

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Published

2024-12-24

How to Cite

1.
Jindapol1 J, Bunlear B, Poldongnok P, Siripaopradit P, Shiraarnon S, Phukmee K, Sengkunpai L. Anticancer potential of Moringa oleifera aqueous leaf extract on human cholangiocarcinoma cells . Health Sci Tech Rev [internet]. 2024 Dec. 24 [cited 2025 Mar. 17];17(3):32-4. available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/264663

Issue

Vol. 17 No. 3 (2024): September - December 2024

Section

Research articles

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