Effects of medium components on in vitro multiplication efficiency of Siam tulip
คำสำคัญ:
Cytokinins, Shoot multiplication, Concentration of mediumบทคัดย่อ
Siam tulip (Curcuma alismatifolia) is a significant economic ornamental plant in Thailand. A commercial production of Siam tulip should mainly use disease-free rhizome to avoid disease accumulation, which could affect the export of Siam tulip’s rhizomes. Therefore, the enhancing efficiency of tissue culture of Siam tulip for disease-free and quality-consistent in plant production is another approach to improve the quality of Siam tulip production for export capacity. This study aimed to evaluate the effects of cytokinins and nutrient concentrations in MS medium, by using shoots harvested from cv. Red Shadow and cv. Khao Yai as explant for the experiments. The results showed that the 4 mg/L of N6-benzylaminopurine (BAP) resulted in the highest shoot multiplication of cv. Khao Yai and 5 mg/L of BAP gave the highest shoot multiplication of cv. Red Shadow. While Kinetin resulted in the increasing of length of explants. Moreover, the reduction of nutrient concentration in MS medium to 0.5 times and the increase of sucrose concentration to 2 times or 6% provided the highest shoot multiplication. While reducing the nutrient concentration in MS medium to 0.25 times decreased the growth of both cv. Red Shadow and cv. Khao Yai.
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