Inhibitory effect of Bridelia ovata Decne extract on HepG2 cell migration and invasion stimulated by fibroblast-conditioned media

  • Hifza Baig Applied Biological Sciences Program
  • Penchatr Diskul-Na-Ayudthaya Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210
  • Churat Weeraphan Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210
  • N. Monique Paricharttanakul Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210
  • Jisnuson Svasti 1 Applied Biological Sciences Program, 2 Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210
  • Chantragan Srisomsap Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210
Keywords: Bridelia ovata Decne extract, human liver cancer cell line, HepG2, cytotoxicity

Abstract

Migration and invasion are the critical step during tumor metastasis. Therefore, one of the goals for cancer treatment is to suppress the migration and invasion of cancer cells. Here, the effect of Bridelia ovata Decne (BO) extract on cell migration and invasion was investigated using human liver cancer cell lines (HepG2) as a model system. Crude ethanol extract is obtained from plant extract using speed vacuum apparatus. The cytotoxic activities of ethanolic extract on HepG2 cell line were evaluated using 3-[4, 5-Dimethyl-2- thiazolyl]- 2, 5-diphenyltetrazolium bromide (MTT) assay. In vitro migration and invasion assays were performed using a modified Boyden chambers. The results showed that at 80% survival (IC20) with 47 μg/mL as concentration of BO extract inhibited invasion and migration of HepG2 cells. It could suggest that BO extract might be involved in the cytotoxic activity against HepG2 carcinoma cell.

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Published
2015-03-12
How to Cite
1.
Baig H, Diskul-Na-Ayudthaya P, Weeraphan C, Paricharttanakul NM, Svasti J, Srisomsap C. Inhibitory effect of Bridelia ovata Decne extract on HepG2 cell migration and invasion stimulated by fibroblast-conditioned media. Naresaun Phayao J [Internet]. 2015Mar.12 [cited 2021Sep.19];8(1):6-10. Available from: https://li01.tci-thaijo.org/index.php/journalup/article/view/42655
Section
Research articles