Antioxidant and Anti-acne Causing Bacteria (Cutibacterium acnes) Activities of Essential Oil from Marigold Flower (Tagetes erecta L.)
Keywords:
Marigold Flower, Essential Oil, Antioxidants, Antibacterial ActivityAbstract
The objectives of this research are to study antioxidant activity by DPPH assay, total phenolic content, and anti-acne causing bacteria (C. acnes) activity of essential oil extracted from the seed and petal of marigold flower using disc diffusion assay. The essential oil’s antioxidant activities were tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), and the total phenolic content was tested by Folin-Ciocalteau’s reagent method using gallic acid as a standard. The results found that the essential oil from petal has antioxidant activity. The percentage of free radical inhibition was 50. The total phenolic content of the essential oil extracted from petal was higher than that of seed extracts. The inhibition zone of essential oils from petals was 19.79±2.45 mm. These results show that essential oil from petals has both antioxidant and antibacterial activities. Therefore, this information can be used for further development in medical and cosmetic science.
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Manuela G, Zlata K, Gordana K, Olivera G. Effects of Dietary Marigold Extract on Lutein Content, Yolk Color and Fatty Acid Profile of Omega-3 Eggs. J. Sci. Food. Agric. 2019; 99(5): 2292-2299.
N Rajput, M Naeem, S Ali, Y Rui, W Tian. Effect of Dietary Supplementation of Marigold Pigment on Immunity, Skin and Meat Color and Growth Performance of Broiler Chickens. Braz. J. Poultry. Sci. 2012; 14(4): 233-304.
Rattanaporn P, Manthana M, Arom S. Study on Chemical Compositions of Marigold (Tagetes erecta). The 39th Kasetsart University Annual Conference: Plants, Agricultural Extension and Communication. 2001; 404-409.
Suphod T, Kongsak B, Laddawan P, Onuma C. Phytochemical Contents and Antioxidants Activity of Extracts from Rocco Deep Gold Marigold Flowers (Tagetes erecta L.). J. Phranakhon Rajabhat: Science and Technology. 2022; 17(2): 13-25.
Maša H, Mojca Št, Željko K. Extraction of Lutein from Marigold Flower Petals Experimental Kinetics and Modelling. Food. Sci. Tech. 2008; 41(10): 2008-2016.
Shaziya M, Rubiya R, Bibhu P.P, Vasudha S, Mohd A. Green Extraction of Lutein from Marigold Flower Petals, Process Optimization and Its Potential to Improve the Oxidative Stability of Sunflower Oil. Ultrason. Sonochem. 2022; 85: 105994.
P Kasemwattanarot, S Chuachang, P Ramakul. Extraction of Lutein from Marigold Flower Using Solvent Extraction. International Conference on Engineering and Industrial Technology (ICEIT 2020), Pattaya, Thailand. 2020; 965.
Wisinee P, Phatthanon P, Motonobu Go, Artiwan S. Supercritical Carbon Dioxide Extraction of Marigold Lutein Fatty Acid Esters: Effects of Cosolvents and Saponification Conditions. Sep. Sci. Technol. 2011; 46(4): 605-610.
Qingsong L, Yanting L, Jianwen L, Yunchun W, Shengnan L, Huixi B, Wencheng Z, Zeyu W. Microemulsion-Based Lutein Extraction from Marigold Petals: Process Optimization and Stabilization Evaluation. Ind. Crops. Prod. 2023; 195: 116454.
Neha M, Vishal V. K, Ashwin K. Green Ultrasound-Assisted Extraction and Life Cycle Assessment of Lutein from Marigold Flowers Using Biocompatible Surfactants, Int. J. Chem. React. Eng. 2023; 19-24.
Diana M, Joaquín N.D.H., David V.B., Ramón F.R., Tiziana F., Guillermo R. Bio-Accessibility and Antioxidant Activity of Calendula officinalis Supercritical Extract as Affected by in Vitro Co-Digestion with Olive Oil. J. Agric. Food. Chem. 2016; 64(46): 8828-8837.
Ivan M.S., Ivana M.S.G., Physical, Chemical and Antioxidant Stability Studies of a Topical Formulation Containing Pot Marigold (Calendula officinalis L.) Flowers Extract. Adv. Technol. 2021; 0(1): 11-19.
K.C. Preethi, Girija K, Ramadasan K. Antioxidant Potential of an Extract of Calendula officinalis Flowers in Vitro and in Vivo. Pharm. Biol. 2006; 44(9): 691-697.
Chul H.K., Chul H.K., Sung J.R., Y. C.K. Antioxidant and Skin Anti-Aging Effects of Marigold Methanol Extract, Toxicol. Res. 2018; 34(1): 31-39.
Molly C, Elma B. Anti-Aging Effects of Select Botanicals: Scientific Evidence and Current Trends. Cosmetics. 2018; 5(3): 54.
Chul H.K, Sung J.R, Young C.K. Antioxidant and Skin Anti-Aging Effects of Marigold Methanol Extract. Toxicol. Res. 2018; 34(1): 31-39.
Arya P.G., Fathima R., Nanditha Das P.M, Noufira P.N, N. Tamilselvan. Formulation and Evaluation of Marigold Anti-aging Cream. Int. J. Adv. Eng. Manag. 2023; 5(6): 673-678.
Raquel M, Elisa L, M. Rosa P.O, Jose S.U, Ana M.M, Concentration of Antioxidant Compounds from Calendula officinalis through Sustainable Supercritical Technologies, and Computational Study of Their Permeability in Skin for Cosmetic Use. Antioxidants. 2022; 11(1).
Jamnian C, Shetshutchai N, Jutamas M, Ratri B. Effect of Aqueous Extract of Marigold Flowers on Antioxidants and Inhibition of α-Amylase and α-Glucosidase Activities, Khon Kaen Agr. J. 2019; 47(2): 293-306.
Wason P, Sakaewan O, Kanokwan K, Kongsak B. In Vitro α-Amylase and α-Glucosidase Inhibitory Effects, Antioxidant Activities, and Lutein Content of Nine Different Cultivars of Marigold Flowers. Molecules. 2023; 28: 3314.
Guno S.C, Rachna A, Chandana M. Antidiabetic and Antihyperlipidemic Effect of Hydro-Alcoholic Extract of Calendula officinalis. Int. Res. J. Pharm. 2011; 2(1): 61-65.
Esmaeel E, Saeed S, Rahman T, The Protective Effect of Marigold Hydroalcoholic Extract in STZ-Induced Diabetic Rats: Evaluation of Cardiac and Pancreatic Biomarkers in the Serum. J. Bot. 2016.
Kusmiati, Wiobet C, Fifi A, Rahmi H. Effect Lutein of Marigold Flower (Tagetes erecta L.) on Decreasing Glucose and Malondialdehyde Levels in Alloxan-Induced Blood Mice. International Conference on Biology and Applied Science (ICOBAS), 13–14 March 2019, Malang, Indonesia.
Weiyou W, Honggao X, Hua C, Kedong T, Fuguo L, Yanxiang G. In Vitro Antioxidant, Anti-Diabetic and Antilipemic Potentials of Quercetagetin Extracted from Marigold (Tagetes erecta L.) Inflorescence Residues. J. Food. Sci. Technol. 2016; 53: 2614–2624.
Nellippatta D, Basavan D, Salma K, Divanji M, Hebbani N.S., Tripathy A. Bioactive Compounds from Marigold Processing Waste: Extraction, Isolation, and Antidiabetic Activity, J. Appl. Pharm Sci. 2023; 13(1).
Diva S, Marta S.F, José M.S.L, Maria T.C, Isabel F.A. Anti-Inflammatory Activity of Calendula officinalis L. Flower Extract. J. Cosmet. 2021; 8(2).
V Duran, M Matic, M Jovanovć, N Mimica, Z Gajinov, M Poljacki, P Boza, Results of the Clinical Examination of an Ointment with Marigold (Calendula officinalis) Extract in the Treatment of Venous Leg Ulcers, Comparative Study. Int. J. Issue. React. 2005; 27(3): 101-106.
M Buzzi, F de Freitas, M de Barros Winter, Therapeutic Effectiveness of a Calendula officinalis Extract in Venous Leg Ulcer Healing, J. Wound. Care. 2016; 25(12): 732-739.
Leila M. L. P, Ruy S. L. J, Leonice M. F. T, Marina C. V, José R. P, Neusa M. P. Wound Healing and Anti-Inflammatory Effect in Animal Models of Calendula officinalis L. Growing in Brazil. Evid. Based. Comp. Alternat. Med. 2012; 375671.
Deeksheetha P. V, Gheena S, Pratibha R, Rajeshkumar S, Karthikeyan R. In Vitro Evaluation of Antioxidant and Anti-Inflammatory Potentials of Herbal Formulation Containing Marigold Flower (Calendula officinalis L.) Tea. Cureus. 2023; 15(8).
Korengath C. P, Girija K, Ramadasan K. Anti-Inflammatory Activity of Flower Extract of Calendula officinalis Linn. and Its Possible Mechanism of Action. Indian. J. Exp. Biol. 2009; 47(2): 113-20.
Fabiana V. S, Valdete O. S, Rodrigo N. S, Rafael C. S. Effectiveness of Skin Protectors and Calendula officinalis for Prevention and Treatment of Radiodermatitis: an Integrative Review, Review Rev. Bras. Enferm. 2020.
Shihab S, Margaret A. M, Andrew D. V, Eileen G, Robyn C, Scott C, Michael P. Efficacy of Topical Calendula officinalis on Prevalence of Radiation-Induced Dermatitis: A Randomized Controlled Trial. Australas. J. Dermatol. 2021; 62(1): 35-40.
De Cedrón M. G, Mouhid L, García-Carrascosa E, Fornari T, Reglero G, De Molina A. R. Marigold Supercritical Extract as Potential Co-Adjuvant in Pancreatic Cancer: The Energetic Catastrophe Induced via BMP8B Ends up with Autophagy-Induced Cell Death, Front. Bioeng. biotechnol. 2019.
Mouhid L, De Cedrón M. G, Vargas T, García-Carrascosa E, Herranz N, García-Risco M. R, Reglero G., Fornari T, De Molina A. R. Identification of Antitumoral Agents Against Human Pancreatic Cancer Cells from Asteraceae and Lamiaceae Plant Extracts. BMC Compl. Alt. Med. 2018; 18(1).
Sak K, Nguyen H. T. H, Thao D. T, Raal A. Cytotoxic Effect of Chamomile (Matricaria recutita) and Marigold (Calendula officinalis) Extracts on Human Melanoma SK-MEL-2 and Epidermoid Carcinoma KB Cells. Cogent. Med. 2017; 4(1): 1333218.
Abdoul-Latif F. M, Elmi A, Mérito A, Nour M, Risler A, Ainane A, Bignon J, Ainane T, Essential Oils of Tagetes minuta and Lavandula coronopifolia from Djibouti: Chemical Composition, Antibacterial Activity and Cytotoxic Activity against Various Human Cancer Cell Lines. Int. J. Plant. Biol. 2022; 13(3): 315–329.
Jiménez-Medina E, García-Lor A, Paco L, Algarra I, Collado A, Garrido F. A New Extract of the Plant Calendula officinalis Produces a Dual in Vitro Effect: Cytotoxic Anti-tumor Activity and Lymphocyte Activation. BMC Cancer. 2006; 6(1).
Kanathip S, Apitsada S, Kanokpich W, Asamaporn M, The Comparison of Phenolic Compounds, Flavonoids and Antioxidant Activities of the Ethanolic Extracts of Shoots, Leaves, Fruit and Seeds of Leucaena leucocephala. Naresuan Phayao J. 2020; 13(3): 66-73.
Andressa B, Gisely C. L, João C. P. M. Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium brasiliense L. 2013; 18(6): 6852–6865.
Amornrat S, Kanlayaporn J, Srisuda H. The Study of Antibacterial in Weed Extracts. VRU Research and Development Journal of Science and Technology. 2016; 1: 69-72.