ฤทธิ์ต้านอนุมูลอิสระและฤทธิ์ต้านแบคทีเรียก่อสิว (Cut bacterium acnes) ของน้ำมันหอมระเหยที่สกัดจากเมล็ดและกลีบดอกดาวเรือง (Tagetes erecta L.)
DOI:
https://doi.org/10.14456/kcsj.2024.5คำสำคัญ:
ดอกดาวเรือง, น้ำมันหอมระเหย, สารต้านอนุมูลอิสระ, ฤทธิ์ต้านเชื้อแบคทีเรียบทคัดย่อ
งานวิจัยนี้มีจุดประสงค์เพื่อศึกษาฤทธิ์ต้านอนุมูลอิสระด้วยวิธี DPPH assay ปริมาณฟีนอลิกรวม และฤทธิ์ต้านแบคทีเรียก่อสิว (C. acnes) ด้วยวิธี disc diffusion assay จากน้ำมันหอมระเหยที่สกัดจากเมล็ดและกลีบดอกดาวเรืองแห้ง โดยนำน้ำมันหอมระเหยที่ได้ทดสอบฤทธิ์ต้านอนุมูลอิสระด้วย 2,2-diphenyl-1-picrylhydrazyl (DPPH) และทดสอบปริมาณฟีนอลิกรวมด้วย Folin-Ciocalteau’s reagent โดยใช้กรดแกลลิกเป็นสารมาตรฐาน ผลการศึกษาพบว่าน้ำมันหอมระเหยที่สกัดจากกลีบดอกดาวเรืองมีฤทธิ์ต้านอนุมูลอิสระ ซึ่งมีร้อยละการยับยั้งอนุมูลอิสระเท่ากับร้อยละ 50 และพบว่ามีปริมาณฟีนอลิกรวมมากกว่าน้ำมันหอมระเหยที่สกัดจากเมล็ดดาวเรือง ผลการทดสอบฤทธิ์ต้านแบคทีเรียก่อสิวด้วยวิธี disc diffusion assay พบว่า น้ำมันหอมระเหยที่สกัดจากกลีบดาวเรืองมีพื้นที่การยับยั้ง 19.79±2.45 จากการศึกษานี้พบว่าน้ำมันหอมระเหยที่สกัดจากกลีบดาวเรืองมีฤทธิ์ต้านอนุมูลอิสระและฤทธิ์ต้านแบคทีเรียก่อสิวซึ่งสามารถนำข้อมูลจากการวิจัยนี้ไปพัฒนาในทางการแพทย์และวิทยาศาสตร์เครื่องสำอางต่อไป
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