Genotyping of Acanthamoeba spp. from Soil in Narathiwat Province, Thailand

Authors

  • Kruawan Hounkong Princess of Naradhiwas University
  • Peerapon Sornying
  • Jirayu Buatong
  • Nongyao Sawangjaroen

Keywords:

Free-living amoeba, Acanthamoeba spp, Soil, Narathiwat, Genotype

Abstract

Acanthamoeba spp. are free-living amoeba which are widely distributed worldwide. Some genotypes are known to cause diseases in corneal and central nervous system. In the present study, Acanthamoeba spp. (44 isolates) obtained by culture and identify under microscope from soil in 3 districts (Bacho, Yi-ngo, and Ra-ngae) of Narathiwat province from our earlier survey were used. The identification and genotyping of Acanthamoeba spp. were performed by PCR reactions and DNA sequencing. The results showed that 34.1% (15/44) were positive for Acanthamoeba spp. and belong to genotypes T3 (3 isolates), T4 (5 isolates), T5 (4 isolates), T17 (1 isolate) and T18 (2 isolates). Genotypes T3, T4, T5 and T18 have been reported to associate with human diseases. This is the first report proving the contamination of pathogenic Acanthamoeba spp. in soil from Narathiwat province.

References

Adamska, M. (2015). Molecular characterization of Acanthamoeba spp. occurring in water bodies and patients in Poland and redefinition of polish T16 genotype. Journal of Eukaryotic Microbiology, 63, 262-270.

Bunsuwansakul, C., Mahboob, T., Hounkong, K., Laohaprapanon, S., Chitapornpan, S., Jawjit, S., …, Nissapatorn, V. (2019). Acanthamoeba in Southeast Asia-Overview and Challenges. The Korean Journal of Parasitology, 57(4), 341-357.

Casaro, D., Walochnik, W., Kohsler, M., & Rott, M.B. (2015). Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli sp.nov. (genotype T19). Parasitology Research, 114, 2481-2490.

Coronado-Velazquez, D., Silva-Olivares, A., Castro-Munozledo, F., Lares-Jimenez, L.F., Rodriguez-Anaya, L.Z., Shibayama, M., & Serrano-Luna, J. (2020). Acanthamoeba mauritaniensis genotype T4D: An environmental isolate displays pathogenic behavior. Parasitology International, 74, 1-11.

Di cave, D., DAlfonso, R., Comlavi, K.A.D., DOrazi, C., Monno, R., & Berrilli, F. (2014). Genotyping heterogeneity based on 18S-rRNA gene sequence among Acanthamoeba isolation from clinical samples in Italy. Experimental Parasitology, 145, 46-49.

Esboei, B.R., Fakhar, M., Saberi, R., Barati, M., Moslemi, M., Hassannia, H., … Jalallou, N. (2020). Genotyping and phylogenic study of Acanthamoeba isolates from human keratitis and swimming pool water samples in Iran. Parasite Epidemiology and Control, 11, e00164.

Gatti, S., Rama, P., Matuska, S., Berrilli, P., Guerriero, S., Cavallero, A., … Cave, D.D. (2010). Isolation and genotyping of Acanthamoeba strain from corneal infection in Italy. Journal of Medical Microbiology, 59, 1324-1330.

Hall, T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.

Hounkong, K., Sukchan, P., Sujiwattanarat, P., Dangmanee, N., & Sornying, P. (2020). Survey of free-living amoeba from soil sources using culture method in Narathiwat province, Thailand. Princess of Naradhiwas University Journal, 13(1), 279-290.

Jercic, M.I., Aguayo, C., Saldarriaga-Cordoba, M., Muino, L., Chenet, A.M., Lagos, J., … Fernandez, J. (2019). Genotyping diversity of Acanthamoeba strain isolated from Chilean patients with Acanthamoeba keratitis. Parasites and Vectors, 12, 58.

Koyun, I., Koloren, Z., Karaman, U., Tsiami, A., & Karanis, P. (2020). Acanthamoeba spp. in river water samples from the Black Sea region, Turkey. Journal of Water and Health, 18(2), 186-199.

Ledee, D. R., Iovieno, A., Miller, D., Mandal, N., Diaz, M., Fell, J., … Alfonsa, E.C. (2009). Molecular identification of T4 and T5 genotypes in isolates from Acanthamoeba keratitis patients. Journal of Clinical Microbiology, 47, 1458-1462.

Lekkla, A., Suttikornchai, C., Bovornkitti, S., & Sukthana, Y. (2005). Free-living ameba contamination in natural hot springs in Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health, 36(4), 5-9.

Maciver, S.K., Asif, M., Simmen, M.W., & Lorenzo-Morales, J. (2013). A systematic analysis of Acanthamoeba genotype frequency correlated with source and pathogenicity: T4 is confirmed as a pathogen-rich genotype. European Journal Protistology, 49, 217-221.

Magnet, A., Henriquens-Gil, N., Galvan-Diaz, A.L., Izquiedo, F., Fenoy, S., & del Aguila, C. (2014). Novel Acanthamoeba 18S rRNA gene sequence type from an environmental isolate. Parasitology Research, 113, 2845-2850.

Matsui, T., Maeda, T., Kusakabe, S., Arita, H., Yagita, K., Morii, E., & Kanakura, Y. (2018). A case report of granulomatous amoebic encephalitis by group 1 Acanthamoeba genotype T18 diagnosed by the combination of morphological examination and genetic analysis. Diagnosis Pathology, 13, 27.

Nacapunchai, D., Kino, H., Ruangsitticha, C., Sriwichai, P., & Ishih, A. (2001). Terada M. A brief survey of free-living amebae in Thailand and Hamamatsu District, Japan. The Southeast Asian Journal of Tropical Medicine and Public Health, 32, 179-182.

Niyyati, M., Lorenzo-Morales, J., Rezaie, S., Rahimi, F., Martin-Navarro, C.M., … Rezaeian, M. (2010). First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran. Experimental Parasitology, 126(1), 89-90.

Sangruchi, T., Martinez, A.J., & Visvesvara, G.S. (1994). Spontaneous granulomatous amebic encephalitis: report of four cases from Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health, 25(2), 309-313.

Schroeder, J.M., Booton, G.C., Hay, J., Niszl, I.A., Seal, D.V., Markus, M.B., … Byers, T.J. (2001). Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge. Journal of Clinical Microbiology, 39, 1903-1911.

Siddiqui, R., & Khan, N. A. (2012). Biology and pathogenesis of Acanthamoeba. Parasite and Vectors, 5, 1-13.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA 6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

Tanveer, T., Hameed, A., Muazzam, A.G., Jun, S.Y., Gul, A., & Matin, A. (2013). Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan. Parasitology Research, 112, 2925-2932.

Thammaratana, T., Laummaunwai, P., & Boonmars, T. (2016). Isolation and identification of Acanthamoeba species from natural water sources in the northeastern part of Thailand. Parasitology Research, 115(4), 1705-1709.

Walochnik, J., Scheikl, U., & Haller-Schober, E. M. (2015). Twenty years of Acanthamoeba diagnostics in Austria. Journal of Eukaryotic Microbiology, 62, 3-11.

Wanachiwanawin, D., Booranapong, W., & Kosrirukvongs, P. (2012). Clinical feature of Acanthamoeba keratitis in contact lens wearers and non-wearers. The Southeast Asian Journal of Tropical Medicine and Public Health, 43(3), 549-556.

Wannasan, A., Chaiwong, P., Bunchoo, M., & Morakote, N. (2009). Occurrence of thermotolerant Naegleria and Acanthamoeba in some natural water sources in Chiang Mai. Chiang Mai Medical Journal, 48, 117-124.

Yaicharoen, R., Ngrenngarmlert, W., Thongmee, P., & Damsaman, W. (2007). Survey of Acanthamoeba spp. in dust from Bangkok and suburban areas. Journal of Associated Medical Sciences, 40(1), 46-53.

Zhao, G., Sun, S., Zhao, J., & Xie, L. (2010). Genotyping of Acanthamoeba isolates and clinical characteristics of patients with Acanthamoeba keratitis in China. Journal of Medical Microbiology, 59, 462-466.

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Published

2022-01-28

How to Cite

Hounkong, K., Sornying, P., Buatong, J., & Sawangjaroen, N. (2022). Genotyping of Acanthamoeba spp. from Soil in Narathiwat Province, Thailand. Princess of Naradhiwas University Journal, 14(1), 33–43. Retrieved from https://li01.tci-thaijo.org/index.php/pnujr/article/view/251433