Skin Moisturizing Efficacy of Hand Cleansing Gel Containing Cissampelos pareira Leaves Extract
Keywords:
Skin moisture, Hand cleansing gel, Cissampelos pareira leavesAbstract
Background and Objectives Cissampelos pareira leaves are a traditional herbal plant, but their utilization in cosmetics is quite limited. This study aimed to extract C. pareira leaves, develop gel formulations, evaluate their stability, and evaluate the moisturizing efficacy of hand sanitizer gel containing moisturizing agents from C. pareira leaves.
Methods The experimental research, a total of 30 participants were selected by purposive sampling. The data collection was obtained through the measurement of skin moisture using a Moist Sense® and assessing participants’ satisfaction after utilizing the products.
Results The results showed that polysaccharides extracted from C. pareira leaves yielded 30.45 ± 1.18% of dry plant weight. Following the preparation of the gel, it was found that all formulas were successfully developed into gel forms. F1 and F2 formulas demonstrated good stability under accelerated conditions. The F2 formula was identified as the most suitable due to its favorable texture and moderate color; therefore, it was selected for further efficacy testing. The result showed that the F2 formula was not contaminated by four pathogenic microorganisms and exhibited 99.99% bactericidal efficacy against Pseudomonas aeruginosa and Staphylococcus aureus within one minute. In relation to the effectiveness of moisturizing properties, skin hydration levels significantly increased at 15, 30, 60, 90, and 120 minutes after application compared to baseline values (p < 0.05). Furthermore, the test formula showed significantly higher average skin moisture content than the control formulation at all measurement periods (p < 0.05). In addition, participants reported a high level of satisfaction with both the test and control formula.
Conclusion and Recommendation Hand cleansing gel containing C. pareira leaves extract is effective in maintaining moisture. The extract should be utilized in developing formulations for other moisturizing products.
References
Berardi, A., Cenci-Goga, B., Grispoldi, L., Cossignani, L., & Perinelli, D. R. (2020). Analysis of commercial hand sanitisers amid CoViD-19: Are we getting the products that we need? An Official Journal of the American Association of Pharmaceutical Scientists, 21, 286. https://doi.org/10.1208/s12249-020-01818-6
British Standards Institution. (2019). Chemical disinfectants and antiseptics — Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas — Test method and requirements (phase 2, step 1) (BS EN 1276:2019). BSI Standards Publication.
Chanpirom, S., Khat-udomkiri, N., Tree-Udom, T., Ditthawutthikul, N., Saewan, N., Vinardell, M. P., & Sripisut, T. (2025). Potential of Cissampelos pareira L. Pectin as a bioactive compound in moisturizing and anti-aging applications. Cosmetics, 12(1), 5. https://doi.org/10.3390/ cosmetics 12010005
International Organization for Standardization. (2015a). Cosmetics — Microbiology — Detection of Pseudomonas aeruginosa (ISO 22717:2015). ISO.
International Organization for Standardization. (2015b). Cosmetics — Microbiology — Detection of Staphylococcus aureus (ISO 22718:2015). ISO.
International Organization for Standardization. (2015c). Cosmetics - Microbiology - Detection of Candida albicans (ISO Standard No. 18416:2015(E)). https://www.iso.org
Jing, J.L., Pei Yi, T., Bose, R.J., McCarthy, J.R., Tharmalingam, N., & Madheswaran, T. (2020). Hand sanitizers: A review on formulation aspects, adverse effects, and regulations. International Journal of Environmental Research Public Health, 17(9), 3326. https://doi.org/ 10.3390/ijerph17093326
Jitcharoentham, A. (2022). The effects of Cissampelos pareira L. extract for common pathogenic bacteria in hospital. Udonthani Hospital Medical Journal, 30(1), 121-130. https://he02.tci-thaijo.org/index.php/udhhosmj/article/view/257565/175450
Khampha-in, V. (2019). Preparation of natural gelling agents from Krungkhema leaves [Master’s thesis]. Mae Fah Luang University.
Lourith, N., Pungprom, S., & Kanlayavattanakul, M. (2021). Formulation and efficacy evaluation of the safe and efficient moisturizing snow mushroom hand sanitizer. Journal of Cosmetic Dermatology, 20(2), 554-560. https://doi.org/10.1111/jocd.13543
Matatiele, P., Southon, B., Dabula, B., Marageni, T., Poongavanum, P., & Kgarebe, B. (2022). Assessment of quality of alcohol-based hand sanitizers used in Johannesburg area during the CoViD-19 pandemic. Scientific Reports, 12(1), 4231. https://doi.org/10.1038/s41598-022-08117-z
Methajit, S. (2017). Development of facial mask containing Cissampelos pareira extract [Master’s thesis]. Mae Fah Luang University.
Ministry of Public Health. (2016). Determining the characteristics of cosmetics that are prohibited from being manufactured, imported or sold in 2016. Author.
Ministry of Public Health. (2020). Determining the characteristics of cosmetics containing alcohol for hand hygiene that are prohibited from being manufactured, imported or sold in 2020. Author.
Minzanova, S. T., Mironov, V. F., Arkhipova, D. M., Khabibullina, A. V., Mironova, L. G., Zakirova, Y. M., & Milyukov, V. A. (2018). Biological activity and pharmacological application of pectic polysaccharides: A review. Polymers, 10(12), 1407. https://doi.org/10.3390/polym 10121407
Nachengtai, N., & Thatbudh, Y. (2007). Effect of pH and temperature on the quality of pectin extracted from Moringa leaves [Master’s thesis]. Khon Kaen University.
Pharmacopeia, U. S. (2009). 62. Microbiological examination of nonsterile products: tests for specified microorganims. In Usp Pharmacists’ Pharmacopeia; United States Pharmacopeial Convention. Rockville.
Rodchua, C. (2009). Dry hand washing product containing moisturizer from okra [Master of Science Thesis]. Mae Fah Luang University.
Rungrojnawakul, T. (2023). Development of polysaccharide extraction methods from Tremella fuciformis for industrial application [Master’s thesis]. Mae Fah Luang University.
Singthong, J., Ningsanond, S., Cui, S. W., & Goff, H. D. (2005). Extraction and physicochemical characterization of Krueo Ma Noy pectin. Food Hydrocolloids, 19(5), 793 - 801. https://doi.org/10.1016/j.foodhyd.2004.09.007
Soonthararuk, S., & Prungraenu, P. (2019). Study of the effectiveness of herbal facial masks using Moringa oleifera leaves and sericin protein extracted from silk cocoon waste [Paper presentation]. The 14th National Academic Conference of the National Higher Education Research Network, Office of the Education Council, Ministry of Education.
Sri Mahasarakham Nursing College. (2019). Summary report of the practical training results of the adult nursing practice course 1. https://misn.pbri.ac.th/FONMIS/public/uploads/faculty/ 1707552663_941f4a9c6b525fb342e6.pdf
Stutz, N., Becker, D., Jappe, U., John, S. M., Ladwig, A., Spornraft-Ragaller, P., Uter, W., & Löffler, H. (2009). Nurses’ perceptions of the benefits and adverse effects of hand disinfection: Alcohol‐based hand rubs vs. hygienic handwashing: A multicentre questionnaire study with additional patch testing by the German Contact Dermatitis Research Group. British Journal of Dermatology, 160(3), 565-572. https://doi.org/10.1111/j.1365-2133.2008.08951.x
Visscher, M., & Randall, R. (2012). Hand hygiene compliance and irritant dermatitis: A juxtaposition of healthcare issues. International Journal of Cosmetic Science, 34(5), 402 - 415. https://doi.org/10.1111/j.1468-2494.2012.00733.x
Yuan, H., Dong, L., Zhang, Z., He, Y., & Ma, X. (2022). Production, structure, and bioactivity of polysaccharide isolated from Tremella fuciformis. Food Science and Human Wellness, 11(4), 1010 - 1017. https://doi.org/10.1016/j.fshw.2022.03.030
Zhang, T., Guo, Q., Xin, Y., & Liu, Y. (2022). Comprehensive review in moisture retention mechanism of polysaccharides from algae, plants, bacteria and fungus. Arabian Journal of Chemistry, 15(10), 104163. https://doi.org/10.1016/j.arabjc.2022.104163
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Princess of Naradhiwas University Journal
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
