Effects of Seed Priming on Germination and Seedling Vigor of Korean Perilla (Perilla frutescens var. frutescens)
Article Sidebar
Main Article Content
Abstract
The objectives of this study were to evaluate the effectiveness of seed priming treatment on Perilla frutescens var. frutescens, a species known for its low germination rate and dormancy which hinders propagation and commercial production. The experiment was designed in a completely randomized design that composed of five treatments with 3 replications, each replication contained 40 seeds and soaking for 15 min such as non-soaking seed (Control), Soaking in tap water at room temperature, potassium nitrate, lemon juice and gibberellic acid. The Results showed that potassium nitrate has the highest germination percentages during 7, 14 days and 21 days with averages of 38.33 %, 75.50 %, and 75.50%, respectively, and also has the highest germination index. Lemon juice shows the results similar to potassium nitrate. Gibberellic acid was less effective in initiating germination but significantly enhanced post-germination growth for stem height. However, no significant differences were observed in root length, leaf length, weight and leaf number. According to the result, potassium nitrate is suitable for stimulating on var. frutescens with low germination rates and lemon juice presents a viable option for organic farming, while gibberellic acid is recommended to promote seedling growth after germination.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Ali, M. M., Javed, T., Mauro, R. P., Shabbir, R., Afzal, I., & Yousef, A. F. (2020). Effect of seed priming with potassium nitrate on the performance of tomato. Agriculture, 10(11), 498. https://doi.org/10.3390/agriculture10110498
Aochen, C., Kumar, A., Jaiswal, S., Puro, K. u., Shimray, P. W., Hajong, S., Sangma, R. H. C., Aochen, S., Langrai, B., Bhattacharjee, B., Jamir, L., Angami, T., Pattanayak, A., & Mishra, V. K. (2023). Perilla frutescens L.: A dynamic food crop worthy of future challenges. Frontiers in Nutrition, 10, 1130927.
Balaguera-López, H. E., Cárdenas-Hernández, J. F., & Álvarez-Herrera, J. G. (2009). Effect of gibberellic acid (GA₃) on seed germination and growth of tomato (Solanum lycopersicum L.). Acta Horticulturae, 821, 141–148. https://doi.org/10.17660/ActaHortic.2009.821.15
Bewley, J. D. & Black, M. (1994). Seeds: Physiology of development and germination (2nd ed.). Plenum Press.
Bray, C. M. (2015). Seed priming as a means of enhancing seed performance. In R. H. Ellis, M. Black, A. Murdoch, & T. D. Hong (Eds.), Handbook of seed technology. Wallingford: CAB International.
Chakrobortty, J., Imran, S., Mahamud, M. A., Sarker, P., & Paul, N.C. (2022). Effect of citric acid (CA) priming and exogenous application on germination and early seedling growth of okra (Abelmoschus esculentus L.) under salinity stress condition. Archives of Agriculture and Environmental Science, 7(3), 318–326.
Chanprasert, W. (2010). Role of nitrate in stimulating seed germination. Faculty of Agriculture, Kasetsart University. [in thai]
Dhyani, A., Rawat, S., & Joshi, B. (2024). A review on nutritional value, functional properties and pharmacological application of Perilla (Perilla frutescens L.). Biomedical and Pharmacology Journal, 17(1), 88–102.
Duangpatra, J. (1977). Laboratory practices in seed technology. Department of Agronomy, Faculty of Agriculture, Kasetsart University. [in thai]
Fu, M. M., Liu, J., Nijabat, A., Alsudays, I. M., Saleh, M. A., Alamer, K. H., Attia, H., Ziaf, K., Zaman, Q. U., & Amjad, M. (2024). Seed priming with potassium nitrate alleviates the high temperature stress by modulating growth and antioxidant potential in carrot seeds and seedlings. BMC Plant Biology, 24, Article 606.
Holloway, P. S. (2011). Seed germination and gibberellic acid (Georgeson Botanical Notes No. 43). University of Alaska Fairbanks, School of Natural Resources and Agricultural Sciences.
Lee, J. H., Kwon, J. W., & Lee, S. Y. (2001). Effect of GA3 on germination of Perilla frutescens var. acuta KUDO under high temperature. Korean Journal of Medicinal Crop Science, 9(3), 198–204.
Mbi, T. K., Ntsomboh, G. O., & Tatah, E. L. (2022). Seed dormancy: Induction, maintenance and seed technology approaches to break dormancy. In Seed Biology Updates. IntechOpen. https://doi.org/10.5772/intechopen.106153.
Mutetwa, M., Mafukidze, B. P., Makaure, B. T., Mubaiwa, T. C., Dendera, O., Tovigepi, E., Mtaita, T., Ngezimana, W., & Chaibva, P. (2023). Priming seeds with potassium nitrate is associated with modulation of seed germination and seedling growth ecology of Cucumis metuliferus. EUREKA: Life Sciences, (4), 23–35. https://doi.org/10.21303/2504-5695.2023.003030
Shim, S. I., Moon, J. C., Jang, C. S., Raymer, P., & Kim, W. (2008). Effect of potassium nitrate priming on seed germination of seashore paspalum. HortScience, 43(7), 2259–2262. https://doi.org/10.21273/HORTSCI.43.7.2259
Sukasem, K., Chulaka, P., & Kaewsorn, P. (2020). Effects of concentration of KNO3 solution and soaking duration during seed priming on germination and vigor of dill (Anethum graveolens L.) seeds. King Mongkut’s Agricultural Journal, 38(3), 280–287.
Teerangkul, K., Kaewsom, P., & Jaruwattanaphan, T. (2020). Effects of KNO3 and GA3 on breaking dormancy of cocklebur (Xanthium strumarium L.) seeds. King Mongkut’s Agricultural Journal, 38(2), 200–207. [in Thai]
The Seed Collection. (n.d.). How to Grow Perilla Seeds. https://www.theseedcollection.com.au/growguide/perilla-seeds
Veeramani, M., Kumar, S., & Lee, J. (2024). A review of phytochemical and pharmacological activity of Perilla frutescens. International Journal of Pharmacy and Pharmaceutical Research, 12(3), 45–59.
Yi, D., Wang, Z., & Peng, M. (2025). Comprehensive review of Perilla frutescens: Chemical composition, pharmacological mechanisms, and industrial applications in food and health products. Foods, 14(7), 1252. https://doi.org/10.3390/foods14071252
Zhang, C. P., He, P., He, J. X., Yu, Z. L., Du, D. D., & Hu, S. J. (2010). Effects on germination characteristics with different treatments for seeds of medicinal plant Perilla frutescens. Chinese Traditional and Herbal Drugs, 41(8), 1361–1365.
