An An Efficiency of DNA Extraction Methods for Green Microalgae
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Abstract
Microalgae have been known to have numerous medicinal and industrial applications. Molecular studies are important in microalgae research and the study requires suitable concentrations of DNA free of contaminants. At present, numerous protocols exist for DNA extraction from microalgae. However, they are either time-consuming, expensive or work only with few species. In this research, various DNA extraction methods were employed to extract a high concentration of algal DNA with the least contaminants. A total of 30 samples of five algal genus were used in this study. Results indicated that out of five extraction methods, the use of SDS resulted to the highest quality and quantity of DNA, followed by the CTAB method. The extracted algal DNAs obtained from SDS, CTAB, and DTAB methods were suitable for PCR amplification of 18S rDNA region. MseI-digestion was also employed to determine the quality of the isolated algal DNA. The genomic DNA of 24 out of 30, 16 out of 30 and 5 out of 30 algal samples that were extracted using SDS, CTAB, and DTAB methods, respectively, could be digested by MseI. On another hand, the use of Triton x-100 and Chelex -100 methods resulted to a poor DNA quality.
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