Inhibitory Effect of Microalgae Crude Extracts on Antifungal in Fruit Rot Disease of Rambutan

Authors

  • Kittiwan Prommit Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,
  • Jidapa Yongkul Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,
  • Miti Jearaphunt Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,
  • Weena Jirattiwarutkul Chaisarn Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,
  • Cheeranun Klommara Kaewruksa Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,
  • Patcharee Lungmann Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,

Keywords:

microalgae, crude extract, inhibition of rambutan fruit rot disease causes

Abstract

Rambutan (Nephelium lappaceum Linn.) is a tropical fruit in Thailand that has economic value in the market, however the fruit rot disease caused by various fungal pathogens such as Aspergillus sp., Colletotrichum sp., Phomopsis sp., and Pestalotiopsis sp. affects both quality and shelf life. Many farmers used chemicals such as copper oxychloride and sulfur to control the disease, but the use of chemicals affects health and environment as well as increases costs. This research aimed to screen the microalgae in the soil collected from Nam Rad Forest Headwaters, Khiri Rat Nikhom District, Surat Thani, and to test the antifungal activity from the crude extracts against fruit rot disease. The screening results showed 8 species of microalgae, including Haematococcus sp., Fischerella sp, Oscillatoria sp., Hapalosiphon sp., Calothrix sp., Anabaena sp., Aphanocapsa sp. and Scytonema sp. and 4 isolates of fungi cause the fruit rot disease in rambutan, including Penicillium sp., Corticium sp., Pestalotiopsis sp., and Lasiodiplodia sp. Microalgae extracted by methanol yielded 4.45 - 5.37 percent, while the ethyl acetate-extracted yielded 4.48 - 5.39 percent. The evaluation of antifungal activity using disc diffusion technique revealed that the extraction from 3 genera of microalgae cultured in BG-11 medium for 12 days, including Oscilatoria sp., Fischerella sp., and Anabaena sp., could inhibit the rambutan fruit rot disease fungi. Oscillatoria sp. extracted with methanol had the most effective inhibition activity against the rambutan fruit rot disease caused by Lasiodiplopia spp. followed by Oscillatoria sp. extracted with ethyl acetate, with a mean inhibition zone of 15.25 and 14.5 mm, respectively. Anabaena sp. extracted with ethyl acetate inhibited the growth of Corticium sp., while Fischerella sp. extracted with methanol inhibited the growth of Lasiodiplopia spp. with a mean inhibition zone of 11.05 mm.

Author Biographies

Kittiwan Prommit, Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,

Surat-Nasan Road, Muang, Surat Thani 84100, Thailand.

Jidapa Yongkul, Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,

Surat-Nasan Road, Muang, Surat Thani 84100, Thailand.

Miti Jearaphunt, Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,

Surat-Nasan Road, Muang, Surat Thani 84100, Thailand.

Weena Jirattiwarutkul Chaisarn, Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,

Surat-Nasan Road, Muang, Surat Thani 84100, Thailand.

Cheeranun Klommara Kaewruksa, Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,

 Surat-Nasan Road, Muang, Surat Thani 84100, Thailand.

Patcharee Lungmann, Biology Department, Faculty of Science and Technology, Surat Thani Rajabhat University,

Surat-Nasan Road, Muang, Surat Thani 84100, Thailand.

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Published

2023-08-25

How to Cite

Prommit, K., Yongkul, J., Jearaphunt, M., Jirattiwarutkul Chaisarn, W., Klommara Kaewruksa, C., & Lungmann, P. (2023). Inhibitory Effect of Microalgae Crude Extracts on Antifungal in Fruit Rot Disease of Rambutan. Recent Science and Technology, 15(2), 361–372. Retrieved from https://li01.tci-thaijo.org/index.php/rmutsvrj/article/view/250218

Issue

Vol. 15 No. 2 (2023): ๋May- August 2023

Section

Research Article

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