Genetic Variation of Long-Whiskered Catfish (Mystus gulio) Population Using Microsatellite DNA for Hatchery Broodstock Management

Authors

  • Chaowalee Jaisuk Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna, Nan Campus, Phu Phiang, Nan 55000, Thailand.
  • Patchara Nithirojpakdee Faculty of Argo-Industrial Technology, Rajamangala University of Technology Tawan-Ok, Chanthaburi Campus, Khao Khitchakut, Chanthaburi 22210, Thailand.
  • Anocha Kiriyakit Faculty of Argo-Industrial Technology, Rajamangala University of Technology Tawan-Ok, Chanthaburi Campus, Khao Khitchakut, Chanthaburi 22210, Thailand.
  • Kitipong Suwannaket Faculty of Argo-Industrial Technology, Rajamangala University of Technology Tawan-Ok, Chanthaburi Campus, Khao Khitchakut, Chanthaburi 22210, Thailand.
  • Piyachat Srisakda Kung Krabaen Bay Royal Development Study Centre, Tha Mai, Chanthaburi 22120, Thailand.
  • Khanoksinee Sirirak Faculty of Science and Technology, Rajamangala University of Technology Suvarnabhumi, Nonthaburi Center, Mueang, Nonthaburi 11000, Thailand.

Keywords:

Long - whiskered catfish, Mystus gulio, microsatellite, genetic variation

Abstract

Long-whiskered catfish (Mystus gulio) is attractive and promoted in aquaculture due to consumer demand, but there is a lack of fry. This study emphasizes on good management for offspring production to support aquaculture. Therefore, operations should possess sufficient population genetics information to effectively manage broodstock for producing the target offspring. Brood-stock samples were collected from a farm located in Chanthaburi province, Thailand. They were divided into two groups: male and female. Six microsatellite loci developed for M. nemurus were used to assess population genetic variation of M. gulio in the study. The results revealed that the markers could be used for cross-species studies with M. gulio. These microsatellites were polymorphisms. M. gulio males and females showed no significant difference in genetic diversity (p > 0.05), and the effective population size (Ne) was infinite. A bottleneck was detected in males, considering that Ne was exceeded. The results indicated that increasing broodstock numbers could enhance genetic diversity. Genetic differentiation between males and females showed significant values (p < 0.05). Consequently, maintaining genetic diversity level and phenotype of domesticated stockrequires careful brood-stocks management to prevent inbreeding, genetic drift, and negative selection. Improvement of broodstock management practices will have a positive long-term impact  of M. gulio production.

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Published

2024-12-25

How to Cite

Jaisuk, C., Nithirojpakdee, P., Kiriyakit, A., Suwannaket, K., Srisakda, P., & Sirirak, K. (2024). Genetic Variation of Long-Whiskered Catfish (Mystus gulio) Population Using Microsatellite DNA for Hatchery Broodstock Management. Recent Science and Technology, 17(1), 257917. retrieved from https://li01.tci-thaijo.org/index.php/rmutsvrj/article/view/257917

Issue

Vol. 17 No. 1 (2025): January - April

Section

Research Article

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