The Correlation of Lecanoric Acid with Atranorin in Lichen Parmotrema tinctorum

Abstract

In this research investigation, the researchers study the correlation of lecanoric acid and atromarin using Pearson’s correlation. The collection of more than twenty-five lichen samples was conducted at different times with different environments at Khao Yai National Park. The technique of high performance liquid chromatography was used to analyze lichen substances. The hypersil C18 column (250 x 4.0 mm, 5μm) under gradient elution and UV detection at λ 254 nm was employed. The two mobile phases used were methanol as solvent B and buffer solution of 1 percent phosphoric acid as solvent A. The program of gradient elution started with 30 percent B at the flow rate of 0.7 milliliter per minute. Then, solvent B was increased at 70 percent within fourteen minutes and at 100 percent within thirty minutes. The analysis showed that lecanoric acid and atranorin did not exhibit correlation. It was also found that the average amount of lecanoric acid and atranorin in the rainy season was higher than in the dry season.