Effects of Tea Extracts for Inhibition of Enteropathogenic Bacteria
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Abstract
The objective of this research was to study the antimicrobial effect of crude extracts obtained from white tea, green tea, black tea and oolong tea against bacteria causing gastrointestinal diseases including; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella flexneri, Klebsiella pneumoniae and Pseudomonas aeruginosa. Agar well diffusion technique was used for cell enumeration and minimum bactericidal concentration (MBC) was determined. The results showed that S. aureus, P. aeruginosa, S. flexneri and S. typhi were inhibited by white tea, oolong tea and green tea extracts at same concentration. Inhibition zone diameter of white tea extract of S. aureus, P. aeruginosa, S. flexneri and S. typhi were 31.50±0.50, 15.50±0.50, 13.58±0.38 and 11.58±0.38 mm, respectively. Inhibition zone diameter of oolong tea extract were 27.33±0.57, 12.33±0.28, 10.83±0.28 and 6.33±0.57 mm. Inhibition zone diameter of green tea extract are 23.67±1.25, 11.67±0.76, 9.50±0.50 and 5.17±0.28 mm., whereas black tea extract did not show any growth inhibition of enteropathogenic bacteria. E. coli and K. pneumonia growths cannot be inhibited by all four tea extracts. The white tea extract showed antibacterial activity on S. aureus, P. aeruginosa, S. flexneri and S. typhi with minimum bactericidal concentration (MBC) of 450, 450, 500 and 500 mg/mL, respectively. Oolong tea extract showed antibacterial activity on four bacteria with MBC of 500, 500, 500 and 500 mg/mL, and green tea extract were showed MBC 600, 500, 500 and 550 mg/mL to inhibit S. aureus, P. aeruginosa, S. flexneri and S. typhi, respectively. The current study thus supported the trend of exploiting antimicrobial effects of white tea, oolong tea and green tea extracts for gastrointestinal disease prevention and therapy.
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References
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