Short-term Storage of White Shrimp (Litopenaeus vannamei) Spermatophores.
Article Sidebar
Main Article Content
บทคัดย่อ
The objective of this study was to estimate the suitability of extender solutions for maintaining sperm viability in spermatophore of white shrimp (Litopenaeus vannamei). L.vannamei sperm were exposed to different types of extenders to evaluate their efficiency over various storage periods. The type of suitable extender solution for shrimp spermatophores was observed in spermatophore stored in mineral oil and Ca-F saline. At 720 hr. extended period, the highest percentage of sperm viability was observed in mineral oil (64.33±17.29). Sperm exposed in Ca-F saline, although percent viability was lower than that in mineral oil after 1 month of storage, there was no significant difference at beginning periods. The highest percentage of sperm mortality was observed in phosphate buffer at 24 hr. storage. However, although in this study the best result of short-term storage of L. vannamei sperm was observed with sperm preserved in mineral oil extender solution, next study for long-term storage (cryopreservation) the Ca-F saline should be considered as a good alternative for long-term storage due to its sperm cell osmoregulation properties.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของ PBRU Science Journal
References
Okomoda VT, Koh ICC, Shahreza SM. A simple technique for accurate estimation of fertilization rate with specific application to Clarias gariepinus (Burchell, 1822). Aquac Res 2017;49:1116–21.
Ferreira GS, Silva VF, Martins MA, Silva ACCP da, Machado C, Seiffert WQ, et al. Strategies for ammonium and nitrite control in Litopenaeus vannamei nursery systems with bioflocs. Aquac Eng 2020;88:102040.
Lotz JM, Soto MA. Model of white spot syndrome virus (WSSV) epidemics in Litopenaeus vannamei. Dis Aquat Organ 2002;50:199-209.
Zeng D, Chen X, Xie D, Zhao Y, Yang C, Li Y, et al. Transcriptome analysis of Pacific white shrimp (Litopenaeus vannamei) hepatopancreas in response to Taura syndrome Virus (TSV) experimental infection. PloS One 2013;8:e57515.
Zorriehzahra MJ, Banaederakhshan R. Early Mortality Syndrome (EMS) as new emerging threat in shrimp industry. Adv Anim Vet Sci 2015;3:64-72.
Rajendran KV, Shivam S, Praveena PE, Rajan JJS, Kumar TS, Avunje S, et al. Emergence of Enterocytozoon hepatopenaei (EHP) in farmed Penaeus (Litopenaeus vannamei) in India. Aquaculture 2016;454:272-80.
Kiatpathomchai W, Jaroenram W, Arunrut N, Gangnonngiw W, Boonyawiwat V, Sithigorngul P. Experimental infections reveal that common Thai crustaceans are potential carriers for spread of exotic Taura syndrome virus. Dis Aquat Organ 2008;79:183-90.
Braga A, Lopes D, Magalhães V, Klosterhoff MC, Romano LA, Poersch LH, et al. Hemocytic melanization in shrimp spermatophores. Aquaculture 2018;486:64-7.
Corral-Rosales DC, Ricque-Marie D, Cruz-Suárez LE, Arjona-López O, Rodríguez-Jaramillo C, Navarro C, et al. Reduction of spermatophore melanization in Litopenaeus vannamei shrimp fed Ulva clathrata during a commercial hatchery production. Anim Reprod Sci 2020;217:106468.
Nimrat S, Sangnawakij T, Vuthiphandchai V. Preservation of black tiger shrimp (Penaeus monodon) spermatophores by chilled storage. J World Aquaculture Soc 2005;26:76-86.
Guthrie HD, Welch GR, Theisen DD, Woods III LC. Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm. Theriogenology 2011;75:951-61.
Kang X, Li G, Mu S, Guo M, Ge S. Acrosome reaction of Chinese mitten-handed crab Eriocheir sinensis (Crustacea: Decapoda) spermatozoa: Promoted by long-term cryopreservation. Aquaculture 2009;295:195–9.
Ishida T, Talbot P, Kooda-Cisco M. Technique for the long-term storage of lobster (Homarus) spermatophores. Gamete Res 1986;14:183–95.
Bugnot AB, Greco LSL. Structural alterations in the male reproductive system of the freshwater crayfish, Cherax quadricarinatus (Decapoda, Parastacidae). J Invertebr Pathol 2009;102:160–6.
Chow S. Artificial insemination using preserved spermatophores in the palaemonid shrimp Macrobrachium rosenbergii. Bull Japan Soc Sci Fish 1982;48:1693-5.
Chow S, Taki Y, Ogasawara Y. Cryopreservation of spermatophore of the freshwater shrimp, Macrobrachium rosenbergii. Biol Bull 1985;168:471-5.
Nimrat S, Noppakun S, Sripuak K, Traimat B, Vuthiphandchai VV. Cryopreservation of banana shrimp (Fenneropenaeus merguiensis) spermatophores with supplementation of medicinal plant extracts: Development of a programmable controlled-rate method and a practical method. Aquaculture 2020;515:734537.
Bart AN, Choosuk S, Thakur DP. Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius). Aquac Res 2006;37:523–8.
Vuthiphandchai V, Nimrat S, Kotcharat S, Bart A. Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophore. Theriogenology 2007;68:1192-9.
Diamond S, Powell A, Shields RJ, Rowley AF. Is spermatophore melanisation in captive shrimp (Litopenaeus vannamei) a result of an autoimmune response? Aquaculture 2008;285:14-8.
Brown A, McVey J, Middleditch BS, Lawrence AL. Maturation of shrimp (Penaeus sertiferous) in captivity. J World Maricult Soc 1979;10:435-44.
Rattanachuay P, Kantachote D, Tantirungkij M, Nitoda T, & Kanzaki H. Inhibition of shrimp pathogenic vibrios by extracellular compounds from a proteolytic bacterium Pseudomonas sp. W3. Electron J Biotechnol 2010;13(1).
Alfaro-Montoya J. The reproductive conditions of male shrimps, genus Penaeus, sub-genus Litopenaeus (open thelyca penaeoid shrimps): A review. Aquaculture 2010;300:1-9.
Pongtippatee P, Vanichviriyakit R, Chavadej J, Plodpai P, Pratoomchart B, Sobhon P, et al. Acrosome reaction in the sperm of the black tiger shrimp Penaeus monodon (Decapoda, Penaeidae). Aquac Res 2007;38:1635-44.
Alfaro J, Komen J, Huisman EA. Cooling, cryoprotectant and hypersaline sensitivity of penaeid shrimp embryos and nauplius larvae. Aquaculture 2001;195:353-66.
Nimrat S, Siriboonlamom S, Zhang S, Xu Y, Vuthiphandchai V. Chilled storage of white shrimp (Litopenaeus vannamei) spermatophores. Aquaculture 2006;261:944-51.
Huang C, Dong Q, Walter RB, Tiersch TR. Sperm cryopreservation of green swordtail Xiphophorus helleri, a fish with internal fertilization. Cryobiology 2004;48:295-308.
Vuthiphandchai V, Chomphuthawach S, Nimrat S. Cryopreservation of red snapper (Lutjanus argentimaculatus) sperm: Effect of cryoprotectants and cooling rates on sperm motility, sperm viability, and fertilization capacity. Theriogenology 2009;72:129-38.
Chomphutawach S, Samosorn T, Juntaban J, Nuangsaeng B, Preechaphol R, Yuvanatemiya V, et al. Evaluation of morphological and ultrastructural changes of black tiger shrimp (Penaeus monodon) spermatophore. IOSR-JESTFT 2015;9:34-40.