Evaluation of Antibacterial Activity and Gel Formulation Development from Terminalia chebula Retz Extract

Abstract

The objective of this study was to prepare the crude ethanol extract of Terminalia
chebula and to evaluate an antibacterial activity of T. chebula extract against dermatitis
inducing bacteria including Staphylococcus aureus and Staphylococcus epidermidis.
Antibacterial activity was tested by agar well diffusion method while minimum inhibitory
concentration required to inhibit the growth of 50% of organisms (MIC50) and minimum
bactericidal concentration (MBC) were tested by broth macrodilution method. The yield of
the crude extract was 14.124% of the dry weight of T. chebula fruit. The results indicated
that T. chebula extract can inhibit the growth of both S. aureus and S. epidermidis. The
MIC50 of S. aureus and S. epidermidis were 0.96 and 0.45 mg/ml, respectively, while MBC
were 2.05 and 0.78 mg/ml, respectively. Gel composed of T. chebula was prepared and its
physiochemical properties were evaluated. The physical properties of gel were clear light
yellow color with pH 6.5 and showed good appearance.