Formulation of Whitening Cosmetics from Lakoocha Extract

Abstract

The aims of this research are to test antityrosinase activity by utilizing dopachrome
method, to evaluate the antioxidative activity by using 1,1-diphenyl-2-picrylhydrazyl
(DPPH) antioxidant assay and to determine the total phenolic content by Folin Ciocalteu
reagent by comparing the gallic acid content of both lakoocha crudes extracted by
propylene glycol and ethanol. We determined the stabilities of cosmetic formulations in
which both crude extracts in propylene glycol and ethanol (lotion mixed with ethanol
extract, lotion mixed with propylene glycol extract, shower cream mixed with ethanol
extract, shower cream mixed with propylene glycol extract,) were added. According to the
results, the extraction by propylene glycol and ethanol gave crude % yields of 12.50 and
11.50, respectively. The results demonstrated that the ethanol extract of lakoocha
exhibited higher antityrosinase activity and 2.5 fold better IC50 compared to that of the
reference tyrosinase inhibitor, kojic acid. In the case of propylene glycol the extract
showed inhibitory activity with IC50 value of 25.60 ± 0.15 μg/mL, however the activity was
not as high as when compared to that of the reference kojic acid. The antioxidative activity
of both propylene glycol and ethanol extracts of lakoocha exhibited the same range of
IC50 values, 0.87 ± 0.05 and 2.04 ± 0.43 μg/mL, respectively. In addition, it was found that
both extract, showed inhibitory activity of 12.19 and 5.2 fold more efficacy than vitamin C.
The total phenolic content of the propylene glycol and ethanol extracts of lakoocha were
836.044 ± 0.21 and 590.083 ± 0.5010.84 mg gallic acid extract (GAE/g extract), respectively.
When mixing the crude extracts into lotion and shower cream, they resulted in the same 

manner but the lotion formula showed higher antioxidative activity and total phenolic
content than those of the shower cream formula. The development of 2 cosmetic
formulas, shower cream and lotion, provided formulations with good physical stability: the
cream texture did not change and spread easily on the skin. Upon experimentation with
various conditions, the viscosity decreased because the emulsion system was not
thermodynamically stable (thermodynamically unstable system).