In Vitro Seed Culture of Dendrobium delacourii Guillaumin
Keywords:Dendrobium delacourii Guillaumin, In Vitro Propagations
The purpose of this study was to assess in vitro propagations of Dendrobium
delacourii Guillaumin. Plantlets were induced from seeds in variable media (MS, ½MS and
¼MS) and sucrose concentrations (0, 15, 20 and 30 g.L-1). Plantlets were cut into 0.5 cm.
and cultured on ½MS medium supplemented with different concentrations of BA (0, 0.5,
1.0, 2.0 and 4.0 mg.L-1) and NAA (0, 0.5 and 1.0 mg.L-1) and cultured for 8 weeks. Results
demonstrated that all treatments were capable of inducing seed germination with 100%
efficacy. The highest plantlet regeneration area (3.77) was obtained with ½MS medium
supplemented with 15 g.L-1 sucrose when cultured for 12 weeks. The ½MS medium
supplemented with BA 4.0 mg.L-1 and NAA 1.0 mg.L-1 could induce the highest number of
shoots (5.25/plantlets). The highest number of leaves (4/shoot) occurred under the ½MS
medium supplemented with BA 4.0 mg.L-1 and NAA 0.5 mg.L-1 condition. The best medium
for root induction was ½MS medium supplemented with BA 2.0 mg.L-1 and NAA 1.0 mg.L-1
resulting in 1.42 roots/ plantlets.