In Vitro Seed Culture of Dendrobium delacourii Guillaumin

Abstract

The purpose of this study was to assess in vitro propagations of Dendrobium
delacourii Guillaumin. Plantlets were induced from seeds in variable media (MS, ½MS and
¼MS) and sucrose concentrations (0, 15, 20 and 30 g.L-1). Plantlets were cut into 0.5 cm.
and cultured on ½MS medium supplemented with different concentrations of BA (0, 0.5,
1.0, 2.0 and 4.0 mg.L-1) and NAA (0, 0.5 and 1.0 mg.L-1) and cultured for 8 weeks. Results
demonstrated that all treatments were capable of inducing seed germination with 100%
efficacy. The highest plantlet regeneration area (3.77) was obtained with ½MS medium
supplemented with 15 g.L-1 sucrose when cultured for 12 weeks. The ½MS medium
supplemented with BA 4.0 mg.L-1 and NAA 1.0 mg.L-1 could induce the highest number of
shoots (5.25/plantlets). The highest number of leaves (4/shoot) occurred under the ½MS
medium supplemented with BA 4.0 mg.L-1 and NAA 0.5 mg.L-1 condition. The best medium
for root induction was ½MS medium supplemented with BA 2.0 mg.L-1 and NAA 1.0 mg.L-1
resulting in 1.42 roots/ plantlets.