Anti-Cariogenic Activity, Cytotoxicity and Chemical Constituents of Zingiber rubens Roxb


  • Wipawan Pukumpuang Program of Biological Sciences, Chiang Rai Rajabhat University
  • Pratya Chaliewchalad Department of Biology, Rambhai Barni Rajabhat University


Zingiber rubens, Antibacterial, Plant Extract, Essential Oil


The objectives of this study were to evaluate the antibacterial activity, cytotoxicity and chemical constituents of Zingiber rubens Roxb. Four parts of the dried plant materials including rhizomes, stems, leaves and fruits were extracted with distilled water and 95% ethanol to obtain crude extracts. The essential oil was derived from the rhizome using steam distillation. After that, the crude extracts and essential oil were screened for antibacterial activity against Lactobacillus casei TISTR 390 and Streptococcus mutans ATCC 25175 using the agar disc diffusion and broth dilution methods. Moreover, the cytotoxicity of the plant extracts was evaluated using MTT assay on Vero cells. The results show that all ethanolic extracts could inhibit the tested bacteria with an inhibition zone ranging from 7.00–8.67 mm and MIC/MBC ranging from 6.25–50 mg/ml. Additionally, the essential oil also effectively inhibited both the bacterial strains with an inhibition zone ranging from 8.17–8.83 mm and MIC/MBC of 250 mg/ml. For in vitro cytotoxic properties, all plant extracts exhibited no toxicity on Vero cells with CC50 between 30.32 - > 1,000 µg/ml. Notably, the essential oil derived from the plant rhizome also revealed no toxicity in vitro with CC 50 of 2.5 µg/ml. Furthermore, the essential oil from the rhizome identified volatile compounds using Gas Chromatography-Mass Spectrometry (GC-MS). Importantly, 2,6,10-Cycloundecatrien-1-one, 2,6,9,9-tetramethyl-, (E,E,E)- or zerumbone is the main compound in essential oil with a % peak area of 20.47 %. Thus, the crude extract and essential oil of this plant could inhibit cariogenic bacteria and display low toxicity on human cells, which may be useful in the development of an antibacterial agent in the future.


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Abdel-Aziz, S. M., Aeron, A., & Kahil, T. A. (2016). Health benefits and possible risks of herbal medicine. In N. Garg, S.M. Abdel-Aziz, & A. Aeron (Eds.), Microbes in Food and Health (pp. 97-116).

Cham, Switzerland: Springer. Abdul, A.B., Abdelwahab, S.I., Al-Zubairi, A.S., Elhassan, M.M., & Murali, S.M. (2008). Anticancer and antimicrobial activities of zerumbone from the rhizomes of Zingiber zerumbut. International Journal of Pharmacology, 4(4), 301-304.

Ahmed, Z.U., Begum, Z.T., Hassan, M.A., Khondker, M., Kabir, S.M.H., Ahmad, M.A.T.A., ... & Haque, E. U. (2008). Encyclopedia of flora and fauna of Bangladesh. Asiatic Society of Bangladesh, Dhaka.

Baker, P. J., Coburn, R. A., Genco, R. J., & Evans, R. T. (1987). Structural determinants of activity of chlorhexidine and alkyl bisbiguanides against the human oral flora. Journal of dental research, 66(6), 1099-1106.

Boik, J. (2001). Natural compounds in cancer therapy (Vol. 25). Princeton, MN: Oregon Medical Press.

Chamorro, E.R., Zambón, S.N., Morales, W.G., Sequeira, A. F., & Velasco, G.A. (2012). Study of the chemical composition of essential oils by gas chromatography. Gas chromatography in plant science, wine technology, toxicology and some specific applications, 1, 307-324.

Clinical and Laboratory Standards Institute, CLSI. (2015). M02-A12 performance standards for antimicrobial disk susceptibility tests; approved standard (12th ed.). Wayne, USA: Clinical and Laboratory Standards Institute.

Costa, S.M., Martins, C.C., Bonfim, M.D.L.C., Zina, L.G., Paiva, S.M., Pordeus, I.A., & Abreu, M.H. (2012). A systematic review of socioeconomic indicators and dental caries in adults. International journal of environmental research and public health, 9(10), 3540-3574.

Dai, D.N., Thang, T.D., Chau, L.T., & Ogunwande, I.A. (2013). Chemical constituents of the root essential oils of Zingiber rubens Roxb., and Zingiber zerumbet (L.) Smith. American Journal of Plant Sciences, 4(01), 7-10.

da Silva, T.M., Pinheiro, C.D., Orlandi, P.P., Pinheiro, C.C., & Pontes, G.S. (2018). Zerumbone from Zingiber zerumbet (L.) smith: a potential prophylactic and therapeutic agent against the cariogenic bacterium Streptococcus mutans. BMC complementary and alternative medicine, 18(1), 301.

Di Nunzio, M., Valli, V., Tomás-Cobos, L., Tomás-Chisbert, T., Murgui-Bosch, L., Danesi, F., & Bordoni, A. (2017). Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives?. BMC complementary and alternative medicine, 17(1), 453.

Freshney, R.I. (2000). Culture of Animal Cells: A Manual of Basic Technique. NY, USA : John Wiley & Sons Inc.

Habsah, M., Amran, M., Mackeen, M.M., Lajis, N.H., Kikuzaki, H., Nakatani, N., ... & Ali, A.M. (2000). Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. Journal of Ethnopharmacology, 72(3), 403-410.

Hamada, S., & Slade, H.D. (1980). Biology, immunology, and cariogenicity of Streptococc mutans. Microbiological reviews, 44(2), 331.

Johnson, C.B., Kazantzis, A., Skoula, M., Mitteregger, U., & Novak, J. (2004). Seasonal, populational and ontogenic variation in the volatile oil content and composition of individuals of Origanum vulgare subsp. Hirtum, assessed by GC headspace analysis and by SPME sampling of individual oil glands. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 15(5), 286-292.

Kantayos, V., & Paisooksantivatana, Y. (2012). Antioxidant activity and selected chemical components of 10 Zingiber spp. in Thailand. Journal of Developments in Sustainable Agriculture, 7(1), 89-96.

Loesche, W. J. (1986). Role of Streptococcus mutans in human dental decay. Microbiological reviews, 50(4), 353-380.

Nontasit, N., Kanlayanapaphon, C., Mekanawakul, M., & Nualmangsar, O. (2015). Taxonomic studies and traditional uses of Zingiberaceae in Khao Luang national park, Nakhon Si Thammarat Province, Thailand. Walailak Journal of Science and Technology, 12(8), 643-658.

Sanpa, S., & Sanpa, S. (2019). Antimicrobial activity of edible plant extracts against skin infection pathogens. Journal of Food Health and Bioenvironmental Science, 12(2), 34-39.

Selwitz, R.H., Ismail, A.I., & Pitts, N.B. (2007). Dental caries. The Lancet, 369(9555), 51-59.

Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction: a review. Internationale pharmaceutica sciencia, 1(1), 98-106.

Van Houte, J. (1994). Role of micro-organisms in caries etiology. Journal of dental research, 73(3), 672-681.

Veiga, N., Aires, D., & Douglas, F. (2016). Dental caries: A review. Journal of Dental and Oral Health, 3(1), 2.

Vieira, D. R., Amaral, F. M., Maciel, M. C., Nascimento, F. R., Libério, S. A., & Rodrigues, V. P. (2014). Plant species used in dental diseases: ethnopharmacology aspects and antimicrobial activity evaluation. Journal of ethnopharmacology, 155(3), 1441-1449.

Wolinsky, L. E. (1994). Caries and cardiology. Oral microbiology and immunology, 341-359.

Yadav, K., & Prakash, S. (2017). Dental caries: A microbiological approach. Journal of Clinical Infectious Diseases & Practice, 2(1), 1-15.

Yob, N. J., Jofrry, S. M., Affandi, M. M. R., Teh, L.K., Salleh, M. Z., & Zakaria, Z. A. (2011). Zingiber zerumbet (L.) Smith: a review of its ethnomedicinal, chemical, and pharmacological uses. Evidence-Based Complementary and Alternative Medicine, 2011, 1-12.

Yu, Z., Li, W., & Liu, F. (2004). Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells. Cancer Letters, 215(2), 159-166.




How to Cite

Pukumpuang, W., & Chaliewchalad, . P. (2023). Anti-Cariogenic Activity, Cytotoxicity and Chemical Constituents of Zingiber rubens Roxb. Journal of Food Health and Bioenvironmental Science, 12(3), 13–19. Retrieved from



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