Antioxidant Activity and Allelopathic Potential of Lippia nodiflora (L.) Michx on Germination and Growth of Neptunia javanica Miq. seeds
Keywords:
Lippia nodiflora (L.) Michx., Neptunia javanica Miq., Antioxidant activity, Allelopathic potentialAbstract
The research objective was to evaluate the antioxidant activity, the allelopathic potential on germination growth of Neptunia javanica Miq. seeds and the total phenolic content (TPC) of crude extracts and fractions from Lippia nodiflora (L.) Michx. In this research, the ethanol crude extracts of leaves (LE), stems (SE) and roots (RE) from L. nodiflora were subjected to study the antioxidant activity by using DPPH free radical scavenging assay and the allelopathic potential on germination seeds of N. javanica. The RE extract had the highest antioxidant activity with IC50 value of 136.87 ppm. The allelopathic effect of LE extract at a concentration of 20 mg/mL completely inhibited the seed germination of N. javanica. The LE fractions were separated by liquid-liquid partition with hexane and ethyl acetate system to afford the leaf hexane fraction (LH), leaf ethyl acetate fraction (LEtOAc) and leaf hydroethanolic fraction (LW). The antioxidant, TPC and allelopathic activities of all fractions were investigated. The antioxidant activity of LW (158.21) and LEtOAc (175.45) fractions were better than those of LE (296.87) extracts, while LH fraction showed the lowest antioxidant activity at 722.08 ppm. The active allelopathic compounds in LEtOAc at a concentration of 10 mg/mL showed complete inhibition of radicle and hypocotyl on germination seed growth and 50% of seed growth inhibition (IC50) was 3.14 mg/mL. Crude extracts and fractions showed more inhibited radicle growth than hypocotyl growth of N. javanica seeds. The TPC of leaves crude extracts and fractions were the highest. Interestingly, the total phenolic content of LEtOAc fraction, was the highest (0.072 mg GAE/g) that was related to the good allelopathic properties, compared with other fractions and crude extracts. Our results indicate that the ethanolic crude extracts and an ethyl acetate fraction of L. nodiflora leaves can be applied to weed control, especially for N. javanica.
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