Optimization of Transethosome by Varying Dark Purple Glutinous Rice Variety Leum Phua (Oryza sativa var. glutinosa) Extracts and Rice Bran Oil under Hot Method
Keywords:Transethosome, Nanoparticle, Rice extract, Leum Phua glutinous rice
Leum Pua is native Thai glutinous rice that contains antioxidants higher than other colored rice. The purpose of this study was to develop transethosomes (TEs) extracted from Leum Phua rice by hot method. The influence of rice extract and rice bran oil concentrations was investigated on physical properties of the TEs. The formulation of TEs was performed by selecting the optimal ratio obtained from a mixture of glutinous rice extract at the concentration 20, 30 and 40% (w/v) and rice bran oil 20, 25 and 30% (w/v). Characterization of the TEs was based on results from particle size, polydispersity index, zeta potential, entrapment efficiency and stability testing. Leum Pua glutinous rice was extracted by 95% ethanol. The extracts were developed for TEs using rice bran oil as phospholipid and lecithin as permeation enhancer, Span 80 and Tween 20 as edge activator (surfactant) in their formula. Furthermore, the required size of ethosome vesicles was prepared by using sonication. Results showed that the optimized TEs formulation with particle size below 300 nm could be achieved by using rice extract at the concentration 30 and 40% (w/v) and rice bran oil 20% (w/v). At a concentration of rice extract 40% (w/v) and rice bran oil 20% (w/v) showed the highest entrapment efficiency (68.94±2.9%). The good colloidal characteristics had a particle size (PZ) of 233.0±12.9 nm, polydispersity index (PDI) of 0.314±0.078 and zeta potential (ZP) of -51.4±0.24 mV. The TEs optimized formulation was stable at room temperature and also at elevated temperature conditions (45°C/75% RH) for 3 months. Therefore, based on the current study, the potential of employing the novel carrier transethosomalloaded nanoparticles of Leum Phua rice extracts could serve as an effective dermal delivery. The development of TEs for topical delivery can lead to value added of native Thai glutinous rice.
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