Total Phenolics, Flavonoids, DPPH Radical Scavenging and Tyrosinase Inhibition Activities of Sacha inchi (Plukenetia volubilis L.)
Keywords:Sacha inchi, Phenolics, Flavonoids, DPPH radical scavenging, Tyrosinase inhibition
Sacha inchi (Plukenetia volubilis L.) or Inca star is a medicinal plant whose fruits and seeds are mainly used in processing. The objectives of this research were to analyze the total phenolic and flavonoid contents of Sacha inchi from leaves, vines and shells and to study the efficiency of DPPH radical scavenging activities and tyrosinase inhibition activities of Sacha inchi. The leaves, vines and shells were extracted with 95% ethanol using the maceration technique. The crude extracts of leaves, vines and shells were analyzed in terms of total phenolic contents using the Folin-Ciocalteu method, whereas the total flavonoid contents were analyzed using aluminium chloride colorimetric method. The results of the percentage yield in leaves, vines and shells were found to be 19.4815 ±0.0617, 4.81 ± 0.0482 and 2.51 ± 0.0943, respectively. The total phenolic contents in leaves, vines, and shells were 26.3441±0.8895, 37.0925±0.8898 and 30.5202±1.9938 mg of gallic acid/g sample, respectively. The total flavonoid contents were 407.6190±2.5036, 171.2516±3.6696, and 179.0318±3.7771 mg of rutin/g sample, respectively. DPPH radical scavenging activities in leaves, vines and shells were shown with EC50 as 0.0092±0.0090, 0.0803±0.0063 and 0.2527±0.1105 mg/mL, respectively, compared to standard BHT with EC50 as 0.1296±0.0528 mg/ml. The tyrosinase inhibition activities in leaves, vines and shells were shown with IC 50 as 0.0016±0.0107, 0.4924±0.1500 and 0.5986±0.2751 mg/mL, respectively, compared to standard kojic acid with IC50 as 0.0002±0.0011 mg/mL. All selected parts of the Sacha inchi had phenolic and flavonoid contents. The DPPH radical scavenging activities in Sacha inchi extracts were found in leaves, vines and shells. In addition, the tyrosinase inhibition activities were found mainly in leaves, although to a lesser degree in vines and shells of Sacha inchi.
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