Effects of Whey Protein Isolate Mixed with Vitamin D Emulsion Gels on Improving Muscle and Performance Recovery in Resistance-Trained Males

Authors

  • Jutawan Nuanchankong Department of Nutrition and Dietetics, Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage, Pathumthani, 13180 Thailand
  • Pattamaporn Jaroennon Department of Nutrition and Dietetics, Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage, Pathumthani, 13180 Thailand
  • Sakunta Manakla Department of Nutrition and Dietetics, Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage, Pathumthani, 13180 Thailand

Keywords:

Whey Protein Isolate, Vitamin D, Muscle Mass, Fat-Free Mass, Performance Recovery

Abstract

Exercise performance, skeletal muscle repair, and physical recovery are influenced by protein and vitamin D. This study investigated the effects of whey protein isolate mixed with vitamin D emulsion gels (WPD-emulsion gels) on body composition and performance recovery in resistance-trained males. Various formulas of WPD-emulsion gels were developed with an optimal daily dose recommended protein content of 28 g from whey protein isolate, 1000 IU of vitamin D and three different amounts of rice bran oil and gelatin. A 9-point hedonic scale study was conducted to identify the highest rating formula on sensory acceptability and the effects on body composition and performance recovery. Eight resistance-trained males aged between 20-40 years old who spent at least 70% of total exercise time on resistance training for 3-5 days per week were chosen to participate. Participants were assigned WPD-emulsion gels consumed 30 min after resistance training (4 days/week) for 4 weeks. The study instruments were as follows: anthropometric and dietary assessments, the push-up and sit-to-stand test, muscle recovery times, perceived recovery status scale and rating of perceived exertion. Statistical analysis used one-way ANOVA and Paired t-tests. The results showed that the 5% rice bran with 14% gelatin formula received the highest sensory acceptability score. Skeletal muscle mass (at week 0: 59.9±3.4 kg, at week 4: 61.4±3.0 kg) and fat free mass (at week 0: 63.1±3.6 kg, at week 4: 64.6±3.1 kg) were significantly increased from week 0 (p<0.05). In addition, participant’s legs muscle mass and exercise recovery were also significantly improved. The study recommends that the consumption of WPD emulsion gels is beneficial to muscles and could increase fat-free mass and improve exercise performance recovery.

References

Chen, H., Mao, L., Hou, Z., Yuan, F., & Gao, Y. (2020). Roles of additional emulsifiers in the structures of emulsion gels and stability of vitamin E. Food Hydrocoll, 99, 105372.

Chen, Y., Liang, Y., Guo, H., Meng, K., Qiu, J., & Benardot, D. (2022). Muscle-Related Effect of Whey Protein and Vitamin D3Supplementation Provided before or after Bedtime in Males Undergoing Resistance Training. Nutrients, 14(11), 1-18.

Dickinson, E. (2012). Emulsion gels: The structuring of soft solids with protein-stabilized oil droplets. Food Hydrocolloids, 28(1), 224-241.

Dzik, K.P., & Kaczor, J.J. (2019). Mechanisms of vitamin D on skeletal muscle function: oxidative stress, energy metabolism and anabolic state. Eur J Appl Physiol, 119(4), 825–839.

Gao, C., Li, Y., Pan, Q., Fan, M., Wang, L., & Qian, H. (2021). Analysis of the key aroma volatile compounds in rice bran during storage and processing via HS-SPME GC/MS. J. Cereal Sci, 99, 103178.

Gunton, J. E., & Girgis, C. M. (2018). Vitamin D and muscle. Bone Rep,8, 163–167. Institute of Nutrition, Mahidol university. (2018). Nutrient calculation computer software INMUCAL-Nutrients V4 database NB1. Nakornpathom

Institute of Nutrition, Mahidol University. (2018). Nutrient calculation computer software INMUCAL-Nutrients V4 database NB1. Nakornpathom

Jäger, R., Kerksick, C. M., Campbell, B. I., Cribb, P. J., Wells, S. D., Skwiat, T. M., … Purpura, M. (2017). International Society of Sports Nutrition Position Stand: protein and exercise. J Int Soc Sports Nutr, 14(20), 1-25.

Joanisse, S., McKendry, J., Lim, C., Nunes, E.A., Stokes, T., Mcleod, J.C., & Phillips, S.M. (2021). Understanding the effects of nutrition and post-exercise nutrition on skeletal muscle protein turnover: Insights from stable isotope studies. Clin. Nutr. Open Sci.,36, 56-77.

Kim, J., Lee, C., & Lee, J. (2017). Effect of timing of whey protein supplement on muscle damage markers after eccentric exercise. J Exerc Rehabil, 13(4), 436-440.

Latham, C. M., Brightwell, C. R., Keeble, A. R., Munson, B. D., Thomas, N. T., Zagzoog, A. M., … Fry, C. S. (2021). Vitamin D Promotes Skeletal Muscle Regeneration and Mitochondrial Health. Front Physiol, 12, 660498.

Laurent, C. M., Green, J. M., Bishop, P. A., Sjökvist, J., Schumacker, R. E., Richardson, M. T., & Curtner-Smith, M. (2011). A practical approach to monitoring recovery: development of a perceived recovery status scale. J Strength Cond Res, 25(3), 620–628.

Lee, Y. S., Kim, S. D., Kang, H. J., Kim, S. W., Shin, I. S., Yoon, J. S., & Kim, J. M. (2017). Associations of Upper Arm and Thigh Circumferences with Dementia and Depression in Korean Elders. Psychiatry Investig, 14(2), 150–157.

Margolis, L. M., Allen, J. T., Hatch-McChesney, A., & Pasiakos, S. M. (2021). Coingestion of Carbohydrate and Protein on Muscle Glycogen Synthesis after Exercise: A Meta-analysis. Med Sci Sports Exerc, 53(2), 384–393.

Marra, M., Sammarco, R., De Lorenzo, A., Iellamo, F., Siervo, M., Pietrobelli, A., … Donini, L. M (2019). Assessment of Body Composition in Health and Disease Using Bioelectrical Impedance Analysis (BIA) and Dual Energy X-Ray Absorptiometry (DXA): A Critical Overview. Contrast Media Mol Imaging, 2019, 3548284.

McAdam, J. S., McGinnis, K. D., Beck, D. T., Haun, C. T., Romero, M. A., Mumford, P. W., …. Roberson, P. A., (2018). Effect of Whey Protein Supplementation on Physical Performance and Body Composition in Army Initial Entry Training Soldiers. Nutrients, 10(9), 1248.

Omron Healthcare (2020). Full Body Sensor Body Composition Monitor and Scale Model HBF-510. Retrieved January 20, 2023,from https://omronhealthcare.com/wp-content/uploads/hbf-510w-instruction-manual.pdf

Sangwan, S., & Seth, R. (2021). Whey Protein Supplement: An Exclusive Food or Need of the Hour: Review. Annu Res Rev Biol, 36(4), 110-119.

Sangwanna, S., Jaroennon, P., Nuanchankong, J., Suchaiya, W., & Manakla, S. (2019). Body composition among fitness club members of Valaya Alongkorn Rajabhat University under the Royal Patronage Pathum thani Thailand: a cross-sectional study. The Proceeding of the 10th napa 2019, 15-20.

Salvador Castell, G., Serra-Majem, L., & Ribas-Barba, L. (2015). What and how much do we eat? 24-hour dietary recall method. Nutr Hosp, 31(3), 46–48.

Schoenfeld, B.J., & Grgic, J. (2017). Evidence-Based Guidelines for Resistance Training Volume to Maximize Muscle Hypertrophy. Strength Cond J., 40(4),107-12.

Sobral,C., Gomes, D., Silva, M., Martins, P., & Baltazar, A. (2020). Whey protein supplementation in muscle hypertrophy. Eur. J. Public Health, 30(2).

Society of Sensory Professionals (2023). The 9-point Hedonic Scale. Retrieved January 15, 2023, from https://www.sensorysociety.org/knowledge/sspwiki/Pages/The%209point%20Hedonic%20Scale.aspx

Tagawa, R., Watanabe, D., Ito, K., Otsuyama, T., Nakayama, K., Sanbongi, C., … Miyachi, M. (2022). Synergistic Effect of Increased Total Protein Intake and Strength Training on Muscle Strength: A Dose-Response Metaanalysis of Randomized Controlled Trials. Sports Med, 8(1),1-12.

The American College of Sports Medicine (2014). ACSM’s Guidelines for Exercise Testing and Prescription. (9th Ed). Philadelphia : Lippincott Williams & Wilkins.

The American College of Sports Medicine. (2015). Protein Intake for Optimal Muscle Maintenance. Retrieved January 22, 2023, from https://www.acsm.org/docs/default-source/files-for-resource-library/proteinintake-for-optimal-muscle-maintenance.pdf

The Department of Physical Education. Ministry of Tourism and Sports. (2019). Criteria of Physical fitness standard test for aged 19 - 59 years old. Retrieved January,10, 2023 from https://www.dpe.go.th/manual-files411291791796

Trommelen, J., Betz, M. W., & van Loon, L. J.C. (2019). The Muscle Protein Synthetic Response to Meal Ingestion Following Resistance Type Exercise. Sports Med, 49(2), 185–197.

Wilborn, C. D., Taylor, L. W., Outlaw, J., Williams, L., Campbell, B., Foster, C. A., Smith-Ryan, A., Urbina, S., & Hayward, S. (2013). The Effects of Pre- and Post-Exercise Whey vs. Casein Protein Consumption on Body Composition and Performance Measures in Collegiate Female Athletes. J Sci Med Sport, 12(1), 74–79.

Williams, J., Vieira, K., & Williams, S. (2018).The Effects of Supplementing Ingredient Optimized Whey Protein Isolate (ioProtein) Versus Whey Protein Comparator Following High Intensity Exercise for 8-Weeks. J Food Process Technol, 9(2),1-5.

Williams, N. (2017). The Borg Rating of Perceived Exertion (RPE) scale. J Occup Med, 67(5), 404–405.

Van Loon L. J. (2013). Role of dietary protein in post-exercise muscle reconditioning. Nestle Nutr Inst Workshop Ser, 75, 73–83.

Vliet, S. V., Beals, J. W., Martinez, I. G., Skinner, S. K., & Burd, N. A. (2018). Achieving Optimal Post-Exercise Muscle Protein Remodeling in Physically Active Adults through Whole Food Consumption. Nutrients, 10(2),1-14.

Volek, J. S., Volk, B. M., Gómez, A. L., Kunces, L. J., Kupchak, B. R., Freidenreich, D. J., … Aristizabal, J. C. (2013). Whey protein supplementation during resistance training augments lean body mass. J Am Coll Nutr, 32 (2), 122–135.

Downloads

Published

2023-12-19

How to Cite

Nuanchankong, J. ., Jaroennon, P. ., & Manakla, S. . (2023). Effects of Whey Protein Isolate Mixed with Vitamin D Emulsion Gels on Improving Muscle and Performance Recovery in Resistance-Trained Males. Journal of Food Health and Bioenvironmental Science, 16(3), 39–46. Retrieved from https://li01.tci-thaijo.org/index.php/sdust/article/view/261889

Issue

Section

Original Articles