Influence of Various Factors on Hydrolytic Enzyme Activity and COD Removal Efficiency in Cassava Starch Wastewater by Marine Actinomycete Streptomyces sp. A1-3


  • Maliwan Kutako Marine Biotechnology Research Unit, Faculty of Marine Technology, Burapha University, Chanthaburi Campus, Chanthaburi, Thailand
  • Pattana Sillapachai Institute of Marine Science, Burapha University, Chonburi, Thailand
  • Yutthaya Yuyen Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700 Thailand
  • Paveena Tapaneeyaworawong National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand & Center of Excellence for Marine Biotechnology, Department of Marine Science, Chulalongkorn University, Bangkok, Thailand
  • Nisa Siranonthana Institute of Marine Science, Burapha University, Chonburi, Thailand
  • Nongnuch Rungsawang Research and Development Institute, Suan Dusit University, Bangkok, 10300 Thailand
  • Janjarus Watanachote Institute of Marine Science, Burapha University, Chonburi, Thailand


Cassava starch wastewater, COD treatment, Hydrolytic enzyme activity, Marine actinomycetes Streptomyces sp


Wastewater from the production line of cassava starch factories contains a significant amount of organic matter, necessitating treatment before discharging or reusing in the factory. Actinomycetes can produce various hydrolytic enzymes, especially carbohydrate-degrading enzymes, which are important in the biological wastewater treatment process because actinomycetes can utilize several growth substances such as sugar, polysaccharides and protein. In this research, the effects of carbon source, pH, salinity and incubation time on hydrolytic enzyme activity for producing reducing sugar of a marine actinomycete Streptomyces sp. A1-3 were evaluated. The maximum hydrolytic enzyme activity was found in the oatmeal yeast extract carboxymethyl cellulose (OYC) medium with a pH value of 4.0-6.2 and a cultivation time of 4 days. Salinities in the range of 0-35 practical salinity units (psu) had no effect on the hydrolytic enzyme activity of strain A1-3. Subsequently, the efficiency of Streptomyces sp. A1-3 in reducing the chemical oxygen demand (COD) in cassava starch wastewater was evaluated using a Completely Randomized Design (CRD). The control tank had no Streptomyces sp. A1-3, while in the treatment tank, strain A1-3 was added. Aeration was supplied throughout the 16-day experiment. Water samples were collected daily for COD analysis and to measure reducing sugar levels. Within the first 4 days, the efficiency of COD reduction by strain A1-3 was 50%, higher than that in the control tank. During days 7-9, the COD reduction value of strain A1-3 remained almost stable in the range of 58-63%. Adding NaCl


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How to Cite

Kutako, M., Sillapachai, P. ., Yuyen, Y. ., Tapaneeyaworawong, P. ., Siranonthana, N. ., Rungsawang, N. ., & Watanachote, J. . (2024). Influence of Various Factors on Hydrolytic Enzyme Activity and COD Removal Efficiency in Cassava Starch Wastewater by Marine Actinomycete Streptomyces sp. A1-3 . Journal of Food Health and Bioenvironmental Science, 17(1), 31–37. Retrieved from



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