Antioxidant and Anti-acne Activities of Stingless Bee Honey and Propolis Extract
Keywords:
Stingless bee honey, Discriminant, black pepper, Anti-acneAbstract
Stingless bees form a large group of bees that lack a sting and are found in tropical and sub-tropical
areas. Honey and propolis are major products that are produced by the stingless bee. This study aimed to investigate honey and propolis extracts, leading us to isolate bioactive compounds for their antioxidant and anti-acne properties. The four different stingless bee species (Geniotrigona thoracica, Heterotrigona itama, Tetragonula pagdeni, and Lepidotrigona terminate), collected from a community enterprise area (southern Thailand). The raw propolis was extracted via maceration with 20% ethanol. The present study aimed to assess the concentration of phenolics and flavonoids using Folin-Ciocalteu method, Aluminum Chloride Colorimetry method and DPPH respectively, as well as the antibacterial against bacteria Propionibacterium acne (DMST 14917) using the agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined by the in vitro method.The findings indicate that G. thoracica honey has the highest content of total phenolic (30.28±0.51mgGAE/g), flavonoids (59.77±0.05 mg RE/g), and DPPH (1.251±0.27mg/ml), while the phenolic (25.34±0.06 mgGAE/g), flavonoids (44.33±0.41) and DPPH (2.210±0.28) of H. itama propolis extract are higher than those of the other extracts.The antibacterial activity of the honey against P. acne was categorized as highly strong inhibitory, while propolis extract was classified as moderate inhibitory. Additionally, G. thoracica honey exhibits the highest antibacterial activity against P. acnes at 37.74±1.5 mm, MIC (62.5 µg/ml) and MBC (125 µg/ml), while
H. itama propolis extract was 14.43±2.7 mm, MIC (125 µg/ml) and MBC (250 µg/ml). These results indicated that stingless bee honey and propolis extracts contain valuable quantities of phenolic and flavonoid compounds, which serve as natural anti-oxidants and exhibit notable anti-microbial activity. The results suggest that stingless bee products hold commercial potential as a natural source of bioactive ingredients to be formulated into cosmetic agents.
References
Abdullah, N.A., Ja’afar, F., Yasin, H.M., Taha, H., Petalcorin, M.I.R., Mamit, M.H., … Usman, A. (2019). Physicochemical analyses, antioxidant, antibacterial, and toxicity of propolis particles produced by stingless bee Heterotrigona itama found in Brunei Darussalam. Heliyon, 5(9), e02476.
Aboulghazi, A., Bakour, M., Fadil, M., & Lyoussi, B. (2022). Simultaneous optimization of extraction yield, phenolic compounds and antioxidant activity of moroccan propolis extracts: Improvement of ultrasound-assisted technique using response surface methodology. Processes, 10(297), 1-16.
Albaridi, N.A. (2019). Antibacterial potency of honey. International Journal of Microbiology, 2019(1), 1–10.
Almasaudi, S. (2020). The antibacterial activities of honey. Saudi Journal of Biological Sciences, 28(4), 2188–2196.
Alvarez-Suarez, J.M., Giampieri, F., Brenciani, A., Mazzoni, L., Gasparrini, M. González-Paramás, A.M., & Battino, M. (2018). Apis mellifera vs Melipona beecheii cuban polifloral honeys: A comparison based on their physicochemical parameters. Chemical Composition and Biological properties, LWT, 87, 272-279.
Anjum, S.I., Ullah, A., Khan, K.A., Attaullah, M., Khan, H., Ali, H., … Dash, C.K. (2019). Composition and functional properties of propolis (bee glue): A review. Saudi Journal of Biological Sciences, 26(7), 1695-1703.
Araújo, M.J.A.M., Bosco, S.D.M.G., & Sforcin, J.M. (2016). Pythium insidiosum: Inhibitory effects of propolis and geopropolis on hyphal growth. Brazilian Journal of Microbiology, 47(4), 863–869.
Arung, E.T., Syafrizal., Kusuma, I.W., Paramita, S., Amen, Y., Kim, Y-U., Naibaho, N.M., … Shimizu, K. (2023). Antioxidant, anti-inflammatory and anti-acne activities of stingless bee (Tetragonula biroi) propolis. Fitoterapia, 164, 105375.
Ávila, S., Beux, M.R., Ribani, R.H., & Zambiazi, R.C. (2018). Stingless bee honey: Quality parameters, bioactive compounds, health-promotion properties and modification detection strategies. Trends in Food Science and Technology. 81, 37–50.
Barbiéri, C., & Francoy, T.M. (2020). Theoretical model for interdisciplinary analysis of human activities: Meliponiculture as an activity that promotes sustainability. Ambiente & Sociedade, 23, 1-19.
Bachevski, D., Damevska, K., Simeonovski, V.,& Dimova, M. (2020).Back to the basics: Propolis and COVID‐19. Dermatol Therapy, 33(4), e13780.
Biluca, F.C., da Silva, B., Caon, T., Mohr, E.T.B., Vieira, G.N., Gonzaga, L.V., ... Costa, A.C.O. (2020). Investigation of phenolic compounds, antioxidant and antiinflammatory activities in stingless bee honey (Meliponinae). Food Research International, 129, 108756.
Chanchao, C. (2009). Antimicrobial activity by Trigona laeviceps (stingless bee) honey from Thailand. Pakistan Journal of Medical Sciences, 25(3), 364-369.
Chanchao, C. (2013). Bioactivity of honey and propolis of Tetragonula laeviceps in Thailand. in pot-honey (pp. 495-505). Springer, New York, NY.
Costa, A.G Jr., Yoshida, N.C., Garcez, W.S., Perdomo, R.T., Matos, M.D., & Garcez. F.R. (2020). Metabolomics approach expands the classification of propolis samples from midwest Brazil. Journal of Natural Products, 83, 333-343.
Djakaria, S.A., Batubara, I., & Raffiudin, R. (2020). Antioxidant and antibacterial activity of selected indonesian honey against bacteria of acne. Jurnal Kimia Sains dan Aplikasi, 23(8), 267-275.
Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., & Ju, Y.-H. (2014) Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22, 296–302.
Elgayyar, M., Draughon F.A., Golden, D.A., & Mount, J.R. (2001). Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of Food Protection, 64(7), 1019-1024.
Fatima, I.J., Mohd Hilmi, A.B., Salwani, I., & Lavaniya, M. (2018). Physicochemical characteristics of Malaysian stingless bee honey from trigona species. IIUM Medical Journal Malaysia, 17(1), 187–191.
Fletcher, M.T., Hungerford, N.L., Webber, D., Carpinelli de Jesus, M., Zhang, J., Stone, I.S.J., … Zawaw, N. (2020). Stingless bee honey, a novel source of trehalulose: a biologically active disaccharide with health benefits. Scientific Reports, 10(1), 12128.
Gül, A., & Pehlivan, T. (2018). Antioxidant activities of some monofloral honey types produced across Turkey. Saudi Journal of Biological Sciences, 25(6), 1056–1065.
Hernández Zarate, M.S., Abraham Juárez, M.d.R., Cerón García, A., & Ozuna, C. (2018). Flavonoids, phenolic content, and antioxidant activity of propolis from various areas of Guanajuato, Mexico. Food Science & Technology, 38(2), 210–215.
Kamal, M.M., Rashid, M.H.U., Mondal, S.C., El Taj, H.F., & Jung., C.(2019).Physicochemical and microbiological characteristics of honey obtained through sugar feeding of bees. Journal of Food Science and Technology, 56(4), 2267-2277.
Kek, S.P., Chin, N.L., Yusof, Y.A., Tan, S.W., & Chua, L.S. (2014). Total phenolic contents and colour intensity of Malaysian honeys from the Apis spp. and Trigona spp. bees. Agriculture and Agricultural Science Procedia, 2, 150–155.
Klakasikorn, A., Wongsiri, S., Deowanish, S., & Dunagphakdee, O. (2005). New record of stingless bees (Meliponini: Trigona) in Thailand. The Natural History Journal of Chulalongkorn University, 5, 1 – 7.
Kraikongjit, S., Jongjitvimol, T., Mianjinda N., & Sirithep, N. (2018). Antibacterial effect of plant resin collected from Tetrigona apicalis (Smith, 1857) in Thung Salaeng Luang national park, Phitsanulok. Journal of Science and Technology, 15(8), 599–607.
Lim, J.R., Chua, L.S., & Dawood, D.A.S. (2023). Evaluating biological properties of stingless bee propolis. Foods, 12(12), 2290.
Lusby, P.E., Coombes, A.L., & Wilkinson, J.M. (2005) Bactericidal activity of different honeys against pathogenic bacteria. Archives of Medical Research, 36, 464-467.
Martysiak-Żurowska, D., & Wenta, W.A. (2012). Comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk. ACTA Scientiarum Polonorum Technologia Alimentaria, 11(1), 83-5.
Minden-Birkenmaier, B.A. & Bowlin, G.L. (2018). Honey-based templates in wound healing and tissue engineering. Bioengineering, 5(2), 1–27.
Mog, M., Waikhom, D., Panda, S.P., Sharma, S., Ngasotter, S., Tesia, S., … Varshney, S. (2020). Problems of antibiotic resistance associated with oxytetracycline use in aquaculture: A review. Journal of Entomology and Zoology Studies, 8, 1075-1082.
Mokhtar, S.U., Hooi, H.S., Lene, D.T.T., & Jayaraman, S. (2019). Comparison of total phenolic and flavonoids contents in Malaysian propolis extract with two different extraction solvents. International Journal of Engineering Technology and Sciences, 6(2), 1–11.
Moniruzzaman, M., Sulaiman, S.A., Khalil, M.I., & Gan, S.H. (2013). Evaluation of physicochemical and antioxidant properties of sourwood and other malaysian honeys: A comparison with manuka honey. Chemistry Central Journal, 7, 138.
Mohammad, S.M., Mahmud-Ab-Rashid, N-K., & Zawawi, N. (2020). Botanical origin and nutritional values of bee bread of stingless bee (Heterotrigona itama) from Malaysia. Journal of Food Quality. 1-12.
Muhammad, N.I.I., & Sarbon, N.M. (2021). Physicochemical profile, antioxidant activity and mineral contents of honey from stingless bee and honey bee species. Journal of Apicultural Research. 62(2), 1–8.
Mustafa, M.Z., Yaacob, N.S., & Sulaiman, S.A. (2018). Reinventing the honey industry: Opportunities of the stingless bee. Malaysian Journal of Medical Sciences, 25(4), 1–5.
Nasir, N.A.M., Halim, A.S., Singh, K.K.B., Dorai, A.A., & Haneef, M.N.M. (2010) Antibacterial properties of tualang honey and its effect in burn wound management: A comparative study. BMC Complementary and Alternative Medicine, 10(31), 1-7.
Nates-Parra, G. (2001). Stingless bees (Hymenoptera: Apidae: Meliponini) of Colombia. Biotechnology Colombiana, 2, 235 – 242.
Pazin, W.M., Mônaco, L.d.M., Egea Soares, A.E., Miguel, F.G., Berretta, A.A., & Ito, A.S. (2017). Antioxidant activities of three stingless bee propolis and green propolis types. Journal of Apicultural Research, 56(1), 40–49.
Ranneh, Y., Ali, F., Zarei, M., Akim, A.M., Hamid, H.A., & Khazaai, H. (2018). Malaysian stingless bee and Tualang honeys: A comparative characterization of total antioxidant capacity and phenolic profile using liquid chromatography-mass spectrometry, LWT, 89, 1–9.
Rao, P.V., Krishnan, K.T., Salleh, N., & Gan, S.H. (2016). Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Revista Brasileira de Farmacognosia. 26, 657-664.
Salleh, S.N.A.S., Hanapiah, N.A.M., Johari, W.L.W., Ahmad, H., & Osman N.H. (2021). Analysis of bioactive compounds and chemical composition of Malaysian stingless bee propolis water extracts. Saudi Journal of Biological Sciences, 28, 6705–6710.
Santos-Buelga, C., & González-Paramás, A.M. (2017). Chemical composition of honey. In Alvarez-Suarez, J.M., (Ed.) Bee Products Chemical and Biological Properties, (pp. 43-82). NY: Springer International Publishing.
Shamsudin, S., Selamat, J., Sanny, M., Abd., Razak, S.B., Jambari, N.N., Mian, Z., … Khatib, A. (2019). Influence of origins and bee species on physicochemical, antioxidant properties and botanical discrimination of stingless bee honey. International Journal of Food Properties, 22(1), 238–263.
Soleymani, S., Farzaei, M.H., Zargaran, A., Niknam, S., & Rahimi, R. (2020). Promising plant-derived secondary metabolites for treatment of acne vulgaris: a mechanistic review. Archives of Dermatological Research, 312(1), 5–23.
Syafrizal, Ramadhan, R., Kusuma, I.W., Egra, S., Shimizu, K., Kanzaki, M., & Tangkearung, E. (2020).Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity, 21, 4623–4630.
Wattanuruk, D., Phasuk, S., Nilsang, P., & Takolpuckdee, P. (2020). Total phenolics, flavonoids, anthocyanins and antioxidant activities of Khaow-Mak extracts from various colored rice. Journal of Food Health and Bioenvironmental Science, 13(1), 10-18.
Wavinya, F., Omolo, M., Malebo, H., Sifuna, A., Nyongesa, P., & Nonoh, J. (2021). Antibacterial Activity of Honey from Wild Species of Stingless Bees; Plebenia hylderbrandii and Meliponula bocandei. Journal of Biosciences and Medicines, 9(7), 67-84.
Xu, D.P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., … Li, H.-B. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18(96), 1-32.
Zulkifli., N., & Hadi, H.A. (2023). Cosmeceutical benefits of stingless bee honey. Journal of Pharmacy, 3(2), 116- 128.
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