Acetylcholinesterase Inhibition Activity of Ethanolic Extract of Sesbania javanica Miq. Flowers
Keywords:
Alzheimer’s disease, Education Innovation, Fabaceae, Acetylcholinesterase activityAbstract
Cholinergic signaling impairment in Alzheimer’s disease (AD) is associated with a low level of acetylcholine as a result of acetylcholinesterase hydrolysis. Acetylcholinesterase inhibitors are found in numerous plants and phytoconstituents. This study aims to investigate the effect of Sesbania javanica Miq. flower extracts on anti-acetylcholinesterase activity and identify its potential compounds. The ethanolic extracts of Sesbania javanica Miq. flowers showed a relatively high percentage of acetylcholinesterase inhibitory activity in a dose-dependent manner (IC50 = 18.56±1.67 mg/mL). However, physostigmine (reference standard) shows the highest potency of acetylcholinesterase inhibition compared to the extracts, IC50 < 0.005 mg/mL. Gas chromatography-mass spectrometry-based analysis revealed that the ethanolic extract of the flower contained major phytochemicals including thiophene and 1-(5-Bicyclo[2.2.1]heptyl)ethylamine, which are predicted to penetrate across the blood-brain barrier. Thiophene was found to be the most abundant compound of the extract. The molecular docking demonstrated that the active phytochemical 1-(5-Bicyclo[2.2.1]heptyl)ethylamine forms hydrogen bonds with the active site of acetylcholinesterase, exhibiting the same orientation as physostigmine, thereby conferring anti-cholinesterase activity. Our findings suggest that the flower extract of Sesbania javanica Miq. (Fabaceae) may provide new phytochemicals for potential drug discovery as inhibitors of acetylcholinesterase activity.
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