This study was reviewing and collecting fundamental data on biological resources in Nonthaburi suburban area to identify the situation of biological resources within the studied area and to develop the arrangement for the ultimate benefit.
The studied biological resources consisted of protozoa, fireflies, trees, bush trees, collected from 9 stations. Water quality was classified as Beta-Mesosaprobic Zone in saprobic system. BOD was ranged from 2.5-10 mg/L. Waste water was found high organic substances, high level of bacterial biodegradation. Sixty species of protozoa were found, where plant protozoa were more than animal protozoa. The outstanding protozoa species were Euglena and Phacus, which was consistency to the 3rd type of surface water classification standard governed by the Ministry of Science and Technology and Environment 1997. One genus with 2 species of firefly population were found, i.e. Luciola brahmina and Luciola oralis. The most frequencies found were Luciola brahmina, accounted for 90% of all firefly population. The highest density of mature firefly was found in November with the average value of 34 firefly/1,600 square metre. When culturing firefly in the controlled ecological system, it was found that female firefly laid 505±46 eggs with 10-12 days hatching period. The hatching rate was found 82% and 20% proceeded the worm period. The survival rate when developing from pupae to maturity accounted for 1.9%. Regarding trees and bush trees investigation, the number of 3,401 trees and bush trees were found, being 124 species 46 genus. Eighty-one species and 34 families of trees were found. Also, 43 species, 22 genus of bush trees were investigated. The two most frequency found trees were Mangifera indica in Anacadiaceae family and Peltophorum pterocarpum in Papilionaceae fmaily, respectively. The three most frequency found bush trees were Leucaena leucocephala in Mimosaceae family, Ixora sp. in Rubiaceae family and Ficus microcarpa in Moraceae family, respectively. The work on commercial plant propagation network by tissue culture of rare and nearly extinct species found Philodendron had high survival rate and presented high growth with all formula nutrients containing BA. The network foundation gained the high benefit and provided fundamental data for plant propagation services. And yet, it was the excellent resources for skills and knowledge regarding tissue culture of rare and nearly extinct species.