Mutation Induction in Physic nut (Jatropha curcas L.) by Colchicine Treatments
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Published:
Jun 4, 2014
Keywords:
aneuploid energy plant genetic variability mixoploid ploidy chromosome level
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Abstract
The objective of this study aimed to evaluate the genetic variation in physic nut (Jatropha curcas L.) by colchicine induction in two experiments. In Experiment 1, inflorescences of physic nut were treated under different colchicine concentrations by dropping the solution directly at the stage that the frequency of male flower at microspore stage. In Experiment 2, seeds were soaked in different concentrations of colchicine at various durations.
In Experiment 1 and 2, the results showed that the increasing of colchicine concentration likely to decreased size of guard cells, plant height and stomatal density. However, significant different among those parameters was not observed. The ploidy level determined from leaf cells of plants by flow cytometry in both experiments showed almost of plants were disomic (2x). However, in experiment 1, incomplete aneuploidy or mixoploid plants that mixing between trisomic (2x + 1) (at 0 mM colchicine) and tetrasomic (2x + 2) (at 2.5 mM colchicine) with disomic were also found. In experiment 2, incomplete aneuploid plants were composed of trisomic, tetrasomic and pentasomic (2x + 3) with disomic. The mixoploid plant of disomic, trisomic and hexasomic (2x + 4) was also detected when concentrations of colchicine and times for soaking duration were increased.
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Na Chiangmai, P., Pootaeng-on, Y., Meetum, P., Jankomon, N., Muangnoi, D., & Kitthip, D. (2014). Mutation Induction in Physic nut (Jatropha curcas L.) by Colchicine Treatments. Science, Engineering and Health Studies, 8(2), 28–39. https://doi.org/10.14456/sustj.2014.8
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References
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Ambrosi, D. G., Galla, G., Purelli, M., Barbi, T., Fabbri, A., Lucretti, S., Sharbel, T. F., and Barcaccia, G. (2010). DNA marker and FCSS analyses shed light on the genetic diversity and reproductive strategy of Jatropha curcas L. Diversity, 2: 810-836.
Basha, S. D. and Sujatha, M. (2007). Inter and intra-population variability of Jatropha curcas (L.). Characterized by RAPD and ISSR markers and development of population-specific SCAR markers. Euphytica, 156: 375-386.
Bever, J. D. and Felber, F. (1991). The theoretical genetics of autopolyploidy. Oxford Surveys inEvolutionary Biology, 7: 185-217.
Bhattacharya, A., Datta, K., and Kumar, S. D. (2005). Floral biology, floral resource constraints and pollination limitation in Jatropha curcas L. Pakistan Journal of Biological Sciences, 8: 456-460. DOI: 10.3923/pjbs.2005.456.460
Birchler, J. A. (2010). Reflections on studies of gene expression in aneuploids. Biochemical Journal, 426: 119-123.
Birchler, J. A., Bhadra, U., Bhadra, M. P., and Auger, D. L. (2001). Dosage-dependent gene regulation in multicellular eukaryotes: implications for dosage compensation, aneuploidy syndromes, and quantitative traits. Developmental Biology, 234: 275-288.
Carretero, C. L., Cantos, M., and Garcia, J. L. (2007). In vitro-ex vitro salt (NaCl) tolerance of cassava (Manihot esculenta Crantz) plants. In Vitro Cellular and Developmental Biology-Plant, 43: 364-369.
Dasgupta, M., Sahoo, M. R., Kole, P. C., and Mukherjee, A. (2008). Evaluation of orange-fleshed sweet potato (Ipomoea batatas L.) genotypes for salt tolerance through shoot apex culture under in vitro NaCl mediated salinity stress conditions. Plant Cell, Tissue and Organ Culture, 94: 161-170.
Datta, S. K. and Chakrabarty, D. (2009). Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in Chrysanthemum. In Inducted plant mutations in the genomics era (Shu, Q. Y., ed.), pp. 303-305. Food and agriculture organization of the United Nation, Rome.
Doleẑel, J. and Bartoṧ, J. (2005). Plant DNA flow cytometry and estimation of nuclear genome size. Annual of Botany, 95: 99-110.
Fernandez, A. O. and Muzica, B. (1973). Effects of some environmental factors on the differentiation of stomata in Spirodela intermedia W. Koch. Botanical Gazette, 134: 117-121.
Ginting, C. and Maryono, T. (2009). Physic nut (Jatropha curcas L.) diseases in Lampung province. Biotropia, 16: 45-54.
Heller, J. (1996). Physic nut, Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. No 1. International Plant Genetics Resource Institute, Rome. 66 pages.
Henry, I. S., Dilkes, B. P., Miller, E. S., Burkart-Waco, D., and Comai, L. (2010). Phenotypic consequences of aneuploidy in Arabidopsis thaliana. Genetics 186: 1231-1245. DOI: 10.1534/genetics. 110.121079.
Heslop-Harrison, J. S. (1995). Flow cytometry and genome analysis. Probe, 5: 14-17.
Hill, H. D. and Myers, W. M. (1944). Isolation of diploid and tetraploid clones from mixoploid plants of ryegrass (Lolium Perenne L.), produced by treatment of germinating seeds with colchicine. Journal of Heredity, 35: 359-361.
Huang, H. -P., Gao, S. -L., Chen, L. -L., and Wei, K.-H. (2010). In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis. Pharmacognosy Magazine, 6: 51-56.
Inagaki, M. N. (2003). Double haploid production in wheat through wide hybridization. In Doubled haploid production in crop plants (Maluszynski, M., Kasha,K. J., Forster, B. P., and Szarejko, I. (eds.), pp. 53-58. Springer Science + Business Media, New York.
Jenczewski, E., Eber, F., Manzanares-Dauleux, M. J., and Chevre, A. M. (2002). A strict diploidy-like pairing regime is associated with tetrasomic segregation in induced autotetraploids of kala. Plant Breeding, 121: 177-179.
Jongschaap, R. E. E., Corre, W. J., Bindraban, P. S., and Brandenburg, W. A. (2007). Claims and facts on Jatropha curcas L.: Global Jatropha curcas evolution. Breeding and propagation programme. Research Report. [Online URL: https://library.wur.nl/WebQuery/wurpubs/ lang/358549] accessed on January 3, 2012.
Koh, H. -J., Heh, M. -H., and Lee, G. -S. (1993). Transmission of extra chromosomes and its effects on anther culture in rice trisomic plants. In Biotechnology in Agriculture (You, C., Chen, Z., and Ding, Y. (eds.). Current Plant Science and Biotechnology in Agriculture, 15: 313-316.
Kung, C. Y. and Tam, H. C. (2005). On-line management and control of distributed renewable energy power plant part I-Fuzzy modeling of PV sytem. PVSEC-15, Shanghai China.
Lee, E. A., Darrah, L. L., and Coe, E. H. (1996). Dosage effects on morphological and quantitative traits in maize aneuploids. Genome, 39: 898-908.
Li, M. R., Li, H. Q., and Wu, G. J. (2006). Study on factors influencing Agrobacterium-mediated transformation of Jatropha curcas (in Chinese). Journal of Molecular Cell Biology, 39: 83-89.
Mayo, O. (1971). Rates of change in gene frequency in tetrasomic organisms. Genetica, 42: 329-339.
Miller, M., Zhang, C., and Chen, Z. J. (2012). Ploidy and hybridity effects on growth vigor and gene expression in Arabidopsis thaliana hybrids and their parents. G3 (Bethesda), 2: 505-513.
Mishra, M. K. (1997). Stomatal characteristics at different ploidy levels in Coffea L. Annuals of Botany, 80: 689-692.
Moody, M. E., Mueller, L. D., and Soltis, D. E. (1993). Genetic variation and random drift in autotetraploid populations. Genetics, 134: 649-657.
Oates, K. M., Touchell, D. H., and Ranney, T. G. (2013). Induced variation in tetraploid Rudbeckia subtomentosa ‘Henry Eilers’ regenerated from gramma-irradiated callus. Horst Science, 48: 831-834.
Omezzine, F., Ladhari, A., Nefzi, F., Harrath, R., Aouni, M., and Haouala, R. (2012). Induction and flow cytometry identification of mixoploidy through colchicine treatment of Trigonella foenum-graecum L. African Journal of Biotechnology, 11: 16434-16442.
Paepatung, N., Nopharatana, A., and Songkasiri, W. (2009). Bio-methane potential of biological solid materials and agricultural wastes. Asian Journal on Energy and Environment, 10: 19-27.
Peerapong, P. and Limmeechokchai, B. (2009). Exergetic and thermoeconomic analyses of the rice-husk power plant in Thailand. Journal of Metals, Materials and Minerals, 19: 9-14.
Piromya, R. and Kermanee, P. (2013). Occurrence of tetraploidy in cochicine-treated physic nut (Jatrophacurcas Linn.). Kasetsart Journal (Nat. Sci.), 47: 23-29.
Rajore, S. and Batra, A. (2005). Efficient plant regeneration via shoot tip explant in Jatropha curcas. Journal of Plant Biochemistry and Biotechnolgy, 14: 73-75.
Sapra, V. T., Hughes, J. L., and Sharma, G. C. (1975). Frequency, size and distribution of stromata in triticale leaves. Crop Science, 15: 356-358.
Sardana, J., Batra, A., and Ali, D. J. (2000). An expeditious method for regeneration of somatic embryos in Jatropha curcas L. Phytomophology, 50: 239-242.
Sharma, H. K., Shukla, A., Kumar, A., Shukla, A., Choudhary, S. B., and Jatothu, J. L. (2013). Variability and genetic diversity assessment in physic nut (Jatropha curcas L.). Journal of Medical Plants Research, 7: 2380-2391.
Songsri, P., Suriharn, B., Sanitchon, J., Srisawangwong, S., and Kesmala, T. (2011). Effects of gramma radiation on germination and growth characteristics of physic nut (Jatropha curcas L.). Journal of Biological Sciences, 11: 268-274.
Sujatha, M., Makkar, H. P. S., and Becker, K. (2006). Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Regulation, 47: 83-90.
Sujatha, M. and Mukta, N. (1996). Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell, Tissue and Organ Culture (Historical Archive), 44: 135-141.
Sujatha, M. and Prabhakaran, A. J. (2003). New ormamental Jatropha hybrids through interspecific hybridization. Genetic Resources and Crop Evolution, 50: 75-82.
Sujatha, M. and Sailaja, M. (2005). Stable genetic transformation of castor (Ricinus communisL.) via Agrobacterium tumefaciens-mediate gene transfer using embryo exes from mature seeds. Plant Cell Report, 23: 803-810.
Tan, G. Y. and Dunn, G. M. (1973). Relationship of stomatal length and frequency and pollen grain diameter to ploidy level in Bromus inermis leyss. Crop Science, 13: 332-334.
Trabi, M., Gübitz, G. M., Steiner, W., and Foidl, N. (1997). Toxicity of Jatropha curcas seeds. In: Proceeding “Jatropha 97”: Biofuels and industrial products from Jatropha curcas. Gübitz, G. M., Mittelbach, M., and Trabi, M. (Eds), Managua, Nicaragua, 23-27 February.
Wei, Q., Lu, W. -D. Liao, Y., Pan, S. L. Xu, Y., Tang, L., and Chen, F. (2004). Plant regeneration from epicotyl explant of Jatropha curcas. Journal of Plant Physiology and Molecular Biology, 30: 475-478.
Winkler, E., Gübitz, G. M., Foidl, N., Staubmann, R., and Steiner, W. (1997). Use of enzymes for oil extraction from Jatropha curcasseeds. In: Proceeding “Jatropha curcas” Gübitz GM, Mittelbach M, Trabi M (eds), Managua, Nicaragua, 23-27 February.
Ambrosi, D. G., Galla, G., Purelli, M., Barbi, T., Fabbri, A., Lucretti, S., Sharbel, T. F., and Barcaccia, G. (2010). DNA marker and FCSS analyses shed light on the genetic diversity and reproductive strategy of Jatropha curcas L. Diversity, 2: 810-836.
Basha, S. D. and Sujatha, M. (2007). Inter and intra-population variability of Jatropha curcas (L.). Characterized by RAPD and ISSR markers and development of population-specific SCAR markers. Euphytica, 156: 375-386.
Bever, J. D. and Felber, F. (1991). The theoretical genetics of autopolyploidy. Oxford Surveys inEvolutionary Biology, 7: 185-217.
Bhattacharya, A., Datta, K., and Kumar, S. D. (2005). Floral biology, floral resource constraints and pollination limitation in Jatropha curcas L. Pakistan Journal of Biological Sciences, 8: 456-460. DOI: 10.3923/pjbs.2005.456.460
Birchler, J. A. (2010). Reflections on studies of gene expression in aneuploids. Biochemical Journal, 426: 119-123.
Birchler, J. A., Bhadra, U., Bhadra, M. P., and Auger, D. L. (2001). Dosage-dependent gene regulation in multicellular eukaryotes: implications for dosage compensation, aneuploidy syndromes, and quantitative traits. Developmental Biology, 234: 275-288.
Carretero, C. L., Cantos, M., and Garcia, J. L. (2007). In vitro-ex vitro salt (NaCl) tolerance of cassava (Manihot esculenta Crantz) plants. In Vitro Cellular and Developmental Biology-Plant, 43: 364-369.
Dasgupta, M., Sahoo, M. R., Kole, P. C., and Mukherjee, A. (2008). Evaluation of orange-fleshed sweet potato (Ipomoea batatas L.) genotypes for salt tolerance through shoot apex culture under in vitro NaCl mediated salinity stress conditions. Plant Cell, Tissue and Organ Culture, 94: 161-170.
Datta, S. K. and Chakrabarty, D. (2009). Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in Chrysanthemum. In Inducted plant mutations in the genomics era (Shu, Q. Y., ed.), pp. 303-305. Food and agriculture organization of the United Nation, Rome.
Doleẑel, J. and Bartoṧ, J. (2005). Plant DNA flow cytometry and estimation of nuclear genome size. Annual of Botany, 95: 99-110.
Fernandez, A. O. and Muzica, B. (1973). Effects of some environmental factors on the differentiation of stomata in Spirodela intermedia W. Koch. Botanical Gazette, 134: 117-121.
Ginting, C. and Maryono, T. (2009). Physic nut (Jatropha curcas L.) diseases in Lampung province. Biotropia, 16: 45-54.
Heller, J. (1996). Physic nut, Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. No 1. International Plant Genetics Resource Institute, Rome. 66 pages.
Henry, I. S., Dilkes, B. P., Miller, E. S., Burkart-Waco, D., and Comai, L. (2010). Phenotypic consequences of aneuploidy in Arabidopsis thaliana. Genetics 186: 1231-1245. DOI: 10.1534/genetics. 110.121079.
Heslop-Harrison, J. S. (1995). Flow cytometry and genome analysis. Probe, 5: 14-17.
Hill, H. D. and Myers, W. M. (1944). Isolation of diploid and tetraploid clones from mixoploid plants of ryegrass (Lolium Perenne L.), produced by treatment of germinating seeds with colchicine. Journal of Heredity, 35: 359-361.
Huang, H. -P., Gao, S. -L., Chen, L. -L., and Wei, K.-H. (2010). In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis. Pharmacognosy Magazine, 6: 51-56.
Inagaki, M. N. (2003). Double haploid production in wheat through wide hybridization. In Doubled haploid production in crop plants (Maluszynski, M., Kasha,K. J., Forster, B. P., and Szarejko, I. (eds.), pp. 53-58. Springer Science + Business Media, New York.
Jenczewski, E., Eber, F., Manzanares-Dauleux, M. J., and Chevre, A. M. (2002). A strict diploidy-like pairing regime is associated with tetrasomic segregation in induced autotetraploids of kala. Plant Breeding, 121: 177-179.
Jongschaap, R. E. E., Corre, W. J., Bindraban, P. S., and Brandenburg, W. A. (2007). Claims and facts on Jatropha curcas L.: Global Jatropha curcas evolution. Breeding and propagation programme. Research Report. [Online URL: https://library.wur.nl/WebQuery/wurpubs/ lang/358549] accessed on January 3, 2012.
Koh, H. -J., Heh, M. -H., and Lee, G. -S. (1993). Transmission of extra chromosomes and its effects on anther culture in rice trisomic plants. In Biotechnology in Agriculture (You, C., Chen, Z., and Ding, Y. (eds.). Current Plant Science and Biotechnology in Agriculture, 15: 313-316.
Kung, C. Y. and Tam, H. C. (2005). On-line management and control of distributed renewable energy power plant part I-Fuzzy modeling of PV sytem. PVSEC-15, Shanghai China.
Lee, E. A., Darrah, L. L., and Coe, E. H. (1996). Dosage effects on morphological and quantitative traits in maize aneuploids. Genome, 39: 898-908.
Li, M. R., Li, H. Q., and Wu, G. J. (2006). Study on factors influencing Agrobacterium-mediated transformation of Jatropha curcas (in Chinese). Journal of Molecular Cell Biology, 39: 83-89.
Mayo, O. (1971). Rates of change in gene frequency in tetrasomic organisms. Genetica, 42: 329-339.
Miller, M., Zhang, C., and Chen, Z. J. (2012). Ploidy and hybridity effects on growth vigor and gene expression in Arabidopsis thaliana hybrids and their parents. G3 (Bethesda), 2: 505-513.
Mishra, M. K. (1997). Stomatal characteristics at different ploidy levels in Coffea L. Annuals of Botany, 80: 689-692.
Moody, M. E., Mueller, L. D., and Soltis, D. E. (1993). Genetic variation and random drift in autotetraploid populations. Genetics, 134: 649-657.
Oates, K. M., Touchell, D. H., and Ranney, T. G. (2013). Induced variation in tetraploid Rudbeckia subtomentosa ‘Henry Eilers’ regenerated from gramma-irradiated callus. Horst Science, 48: 831-834.
Omezzine, F., Ladhari, A., Nefzi, F., Harrath, R., Aouni, M., and Haouala, R. (2012). Induction and flow cytometry identification of mixoploidy through colchicine treatment of Trigonella foenum-graecum L. African Journal of Biotechnology, 11: 16434-16442.
Paepatung, N., Nopharatana, A., and Songkasiri, W. (2009). Bio-methane potential of biological solid materials and agricultural wastes. Asian Journal on Energy and Environment, 10: 19-27.
Peerapong, P. and Limmeechokchai, B. (2009). Exergetic and thermoeconomic analyses of the rice-husk power plant in Thailand. Journal of Metals, Materials and Minerals, 19: 9-14.
Piromya, R. and Kermanee, P. (2013). Occurrence of tetraploidy in cochicine-treated physic nut (Jatrophacurcas Linn.). Kasetsart Journal (Nat. Sci.), 47: 23-29.
Rajore, S. and Batra, A. (2005). Efficient plant regeneration via shoot tip explant in Jatropha curcas. Journal of Plant Biochemistry and Biotechnolgy, 14: 73-75.
Sapra, V. T., Hughes, J. L., and Sharma, G. C. (1975). Frequency, size and distribution of stromata in triticale leaves. Crop Science, 15: 356-358.
Sardana, J., Batra, A., and Ali, D. J. (2000). An expeditious method for regeneration of somatic embryos in Jatropha curcas L. Phytomophology, 50: 239-242.
Sharma, H. K., Shukla, A., Kumar, A., Shukla, A., Choudhary, S. B., and Jatothu, J. L. (2013). Variability and genetic diversity assessment in physic nut (Jatropha curcas L.). Journal of Medical Plants Research, 7: 2380-2391.
Songsri, P., Suriharn, B., Sanitchon, J., Srisawangwong, S., and Kesmala, T. (2011). Effects of gramma radiation on germination and growth characteristics of physic nut (Jatropha curcas L.). Journal of Biological Sciences, 11: 268-274.
Sujatha, M., Makkar, H. P. S., and Becker, K. (2006). Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Regulation, 47: 83-90.
Sujatha, M. and Mukta, N. (1996). Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell, Tissue and Organ Culture (Historical Archive), 44: 135-141.
Sujatha, M. and Prabhakaran, A. J. (2003). New ormamental Jatropha hybrids through interspecific hybridization. Genetic Resources and Crop Evolution, 50: 75-82.
Sujatha, M. and Sailaja, M. (2005). Stable genetic transformation of castor (Ricinus communisL.) via Agrobacterium tumefaciens-mediate gene transfer using embryo exes from mature seeds. Plant Cell Report, 23: 803-810.
Tan, G. Y. and Dunn, G. M. (1973). Relationship of stomatal length and frequency and pollen grain diameter to ploidy level in Bromus inermis leyss. Crop Science, 13: 332-334.
Trabi, M., Gübitz, G. M., Steiner, W., and Foidl, N. (1997). Toxicity of Jatropha curcas seeds. In: Proceeding “Jatropha 97”: Biofuels and industrial products from Jatropha curcas. Gübitz, G. M., Mittelbach, M., and Trabi, M. (Eds), Managua, Nicaragua, 23-27 February.
Wei, Q., Lu, W. -D. Liao, Y., Pan, S. L. Xu, Y., Tang, L., and Chen, F. (2004). Plant regeneration from epicotyl explant of Jatropha curcas. Journal of Plant Physiology and Molecular Biology, 30: 475-478.
Winkler, E., Gübitz, G. M., Foidl, N., Staubmann, R., and Steiner, W. (1997). Use of enzymes for oil extraction from Jatropha curcasseeds. In: Proceeding “Jatropha curcas” Gübitz GM, Mittelbach M, Trabi M (eds), Managua, Nicaragua, 23-27 February.