Extraction, characterization and wound healing property of chitosan extracted from Penaeus indicus shells on mouse fibroblast (L929) cell line
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The current study aimed to characterize the properties of chitosan, extracted from Penaeus indicus, on the mouse fibroblast cell line (L929) for wound healing purposes. Chitosan is well known for its wide variety of biological characteristics. Shrimp exoskeletons were obtained from a local fish market in Chengalpattu, Tamil Nadu. Chitosan was extracted through demineralization, deproteination, and deacetylation processes. It was characterized using X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy. The characterized chitosan was processed into nanoparticles and evaluated for cytotoxicity on cell lines. The cell line was treated with concentrations ranging from 25 to 500 µg/mL for 4 h. The results demonstrated 99% cell viability across all concentrations. The study primarily focuses on evaluating the ability of chitosan to repair wounds. Mouse fibroblast cell lines were seeded on a microtitre plate at a density of 1×10⁵ cells per well. Results indicated that the chitosan-treated groups exhibited significantly greater wound closure, with 10% to 20% more growth compared to the control groups. These findings underscore the potential of chitosan derived from P. indicus shrimp waste as a valuable pharmaceutical compound. Such applications could significantly contribute to the nation’s socioeconomic development.
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