In vitro synergistic efficacy of nystatin combined with geraniol against Candida albicans and Candida glabrata biofilm on acrylic resin
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Abstract
Candida albicans and Candida glabrata predominate in the oral cavities of elderly people with denture stomatitis. Prolonged nystatin treatment can alter the surface properties of acrylic resin, while geraniol can inhibit Candida biofilm without affecting the surface roughness or color of the acrylic resin. This study investigated the efficacy of nystatin/geraniol combinations against C. albicans and C. glabrata biofilms on acrylic resin. Checkerboard assay revealed synergistic activity of nystatin/geraniol combinations. The minimum biofilm eradication concentration (MBEC) and efficacy against Candida biofilm were evaluated by crystal violet assay. The MBEC90 of nystatin against C. albicans and C. glabrata biofilms was 55 and 131 μg/mL, respectively. The MBEC90 of geraniol against C. albicans and C. glabrata biofilms was 330 and 45,367 μg/mL, respectively. A significantly higher percentage of C. albicans biofilm reduction was observed when combining nystatin at 0.25 MBEC with geraniol at 1 MBEC (70.89%) (p<0.05), compared to geraniol monotherapy (42.35%). However, the percentage of C. glabrata biofilm reduction at sub-inhibitory concentrations did not differ from single-agent treatment. In conclusion, nystatin/geraniol combinations had a synergistic effect against C. albicans and C. glabrata, and combinations at sub-inhibitory concentrations can diminish the biomass of Candida biofilms on acrylic resin.
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References
Al-Dwairi, Z. N., Al-Quran, F. A., & Al-Omari, O. Y. (2012). The effect of antifungal agents on surface properties of poly (methyl methacrylate) and its relation to adherence of Candida albicans. Journal of Prosthodontic Research, 56(4), 277–280. https://doi.org/10.1016/j.jpor.2012.02.006
Coco, B. J., Bagg, J., Cross, L. J., Jose, A., Cross, J., & Ramage, G. (2008). Mixed Candida albicans and Candida glabrata populations associated with the pathogenesis of denture stomatitis. Oral Microbiology and Immunology, 23(5), 377–383. https://doi.org/10.1111/j.1399-302x.2008.00439.x
Dabiri, S., Shams-Ghahfarokhi, M., & Razzaghi-Abyaneh, M. (2018). Comparative analysis of proteinase, phospholipase, hydrophobicity and biofilm forming ability in Candida species isolated from clinical specimens. Journal de Mycologie Médicale, 28(3), 437–442. https://doi.org/10.1016/j.mycmed.2018.04.009
Danchik, C., & Casadevall, A. (2021). Role of cell surface hydrophobicity in the pathogenesis of medically-significant fungi. Frontiers in Cellular Infection and Microbiology, 10, Article 594973. https://doi.org/10.3389/fcimb.2020.594973
Figueiredo-Carvalho, M. H. G., de Souza Ramos, L., Barbedo, L. S., de Oliveira, J. C. A., dos Santos, A. L. S., Almeida-Paes, R., & Zancopé-Oliveira, R. M. (2017). Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata. Mediators of Inflammation, 2017(1), Article 8952878. https://doi.org/10.1155/2017/8952878
Gad, M. M., & Fouda, S. M. (2020). Current perspectives and the future of Candida albicans-associated denture stomatitis treatment. Dental and Medical Problems, 57(1), 95–102. https://doi.org/10.17219/dmp/112861
Gupta, P., Gupta, H., & Poluri, K. M. (2021). Geraniol eradicates Candida glabrata biofilm by targeting multiple cellular pathways. Applied Microbiology and Biotechnology, 105(13), 5589–5605. https://doi.org/10.1007/s00253-021-11397-6
Jogalekar, A. P., Ashrit, P., Vijayalakshmi, V., Prathima, P. T., Tejaswini, R. P., Chandana, G. K., & Sadanandan, B. (2014). Comparative study on Candida biofilm quantification methods. International Reviews in Applied Biotechnology and Biochemistry, 2, 133–138.
Kaypetch, R., Rudrakanjana, P., Churnjitapirom, P., Tua-ngam, P., Tonput, P., & Tantivitayakul, P. (2022). Geraniol and thymoquinone inhibit Candida spp. biofilm formation on acrylic denture resin without affecting surface roughness or color. Journal of Oral Science, 64(2), 161–166. https://doi.org/10.2334/josnusd.21-0435
Khan, M. S. A., Malik, A., & Ahmad, I. (2012). Anti-candidal activity of essential oils alone and in combination with amphotericin B or fluconazole against multi-drug resistant isolates of Candida albicans. Medical Mycology, 50(1), 33–42. https://doi.org/10.3109/13693786.2011.582890
Le, P. H., Nguyen, D. H. K., Aburto-Medina, A., Linklater, D. P., Crawford, R. J., MacLaughlin, S., & Ivanova, E. P. (2020). Nanoscale surface roughness influences Candida albicans biofilm formation. ACS Applied Bio Materials, 3(12), 8581–8591. https://doi.org/10.1021/acsabm.0c00985
Lei, Y., Fu, P., Jun, X., & Cheng, P. (2019). Pharmacological properties of geraniol — A review. Planta Medica, 85(1), 48–55. https://doi.org/10.1055/a-0750-6907
Leite, M. C. A., de Brito Bezerra, A. P., de Sousa, J. P., & de Oliveira Lima, E. (2015). Investigating the antifungal activity and mechanism(s) of geraniol against Candida albicans strains. Medical Mycology, 53(3), 275–284. https://doi.org/10.1093/mmy/myu078
Lyn, X., Zhao, C., Yan, Z., & Hua, H. (2016). Efficacy of nystatin for the treatment of oral candidiasis: A systematic review and meta-analysis. Drug Design, Development and Therapy, 10, 1161–1171. https://doi.org/10.2147/dddt.s100795
Malinovská, Z., Čonková, E., & Váczi, P. (2023). Biofilm formation in medically important Candida species. Journal of Fungi, 9(10), Article 955. https://doi.org/10.3390/jof9100955
Marra, J., Paleari, A. G., Rodriguez, L. S., Leite, A. R. P., Pero, A. C., & Compagnoni, M. A. (2012). Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation. Journal of Applied Oral Science, 20(6), 643–648. https://doi.org/10.1590/S1678-77572012000600009
McReynolds, D. E., Moorthy, A., Jabra-Rizk, M. A., & Sultan, A. S. (2023). Denture stomatitis—An interdisciplinary clinical review. Journal of Prosthodontics, 32(7), 560–570. https://doi.org/10.1111/jopr.13687
Meirowitz, A., Rahmanov, A., Shlomo, E., Zelikman, H., Dolev, E., & Sterer, N. (2021). Effect of denture base fabrication technique on Candida albicans adhesion in vitro. Materials, 14(1), Article 221. https://doi.org/10.3390/ma14010221
Monteiro, D. R., Gorup, L. F., Silva, S., Negri, M., de Camargo, E. R., Oliveira, R., Barbosa, D. B., & Henriques, M. (2011). Silver colloidal nanoparticles: Antifungal effect against adhered cells and biofilms of Candida albicans and Candida glabrata. Biofouling, 27(7), 711–719. https://doi.org/10.1080/08927014.2011.599101
Orsi, I. A., Junior, A. G., Villabona. C. A., Fernandes, F. H. C. N., & Ito, I. Y. (2011). Evaluation of the efficacy of chemical disinfectants for disinfection of heat-polymerised acrylic resin. Gerodontology, 28(4), 253–257. https://doi.org/10.1111/j.1741-2358.2010.00400.x
Pappas, P. G., Kauffman, C. A., Andes, D. R., Clancy, C. J., Marr, K. A., Ostrosky-Zeichner, L., Reboli, A. C., Schuster, M. G., Vazquez, J. A., Walsh, T. J., Zaoutis, T. E., & Sobel, J. D. (2016). Clinical practice guideline for the management of Candidiasis: 2016 update by the infectious diseases society of America. Clinical Infectious Diseases, 62(4), e1–e50. https://doi.org/10.1093/cid/civ933
Parker, R. A., Gabriel, K. T., Graham, K. D., Butts, B. K., & Cornelison, C. T. (2022). Antifungal activity of select essential oils against Candida auris and their interactions with antifungal drugs. Pathogens, 11(8), Article 821. https://doi.org/10.3390/pathogens11080821
Pesee, S., Angkananuwat, C., Tancharoensukjit, S., Muanmai, S., Sirivan, P., Bubphawas, M., & Tanarerkchai, N. (2016). In vitro activity of caspofungin combined with fluconazole on mixed Candida albicans and Candida glabrata biofilm. Medical Mycology, 54(4), 384–393. https://doi.org/10.1093/mmy/myv108
Ramage, G., Vande Walle, K., Wickes, B. L., & López-Ribot, J. L. (2001). Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrobial Agents and Chemotherapy, 45(9), 2475–2479. https://doi.org/10.1128/aac.45.9.2475-2479.2001
Sharma, Y., Rastogi, S. K., Saadallah, S. S. A., Fadel, K. A., & Manzoor, N. (2018). Anti-Candida activity of geraniol: Effect on hydrolytic enzyme secretion and biofilm formation. Journal of Pure and Applied Microbiology, 12(3), 1337–1349. https://doi.org/10.22207/JPAM.12.3.36
Singh, S., Fatima, Z., Ahmad, K., & Hameed, S. (2018). Fungicidal action of geraniol against Candida albicans is potentiated by abrogated CaCdr1p drug efflux and fluconazole synergism. PLoS ONE, 13(8), Article e0203079. https://doi.org/10.1371/journal.pone.0203079
Singh, S., Fatima, Z., & Hameed, S. (2016). Insights into the mode of action of anticandidal herbal monoterpenoid geraniol reveal disruption of multiple MDR mechanisms and virulence attributes in Candida albicans. Archives of Microbiology, 198(5), 459–472. https://doi.org/10.1007/s00203-016-1205-9
van der Maarel-Wierink, C. D., Vanobbergen, J. N. O., Bronkhorst, E. M., Schols, J. M. G. A., & de Baat, C. (2013). Oral health care and aspiration pneumonia in frail older people: A systematic literature review. Gerodontology, 30(1), 3–9. https://doi.org/10.1111/j.1741-2358.2012.00637.x
