Extraction of d-limonene from pomelo (Citrus maxima) peel waste using solvent-free microwave process and antioxidant activity analysis

Main Article Content

Boonta Chutvirasakul
Thanapat Riangnam
Wancharoen Kunkamphon
Prakorn Ramakul

Abstract

Solvent-free microwave assisted extraction has been performed as an efficient green technology for the extraction of d-limonene from pomelo peel waste. This environmentally friendly method does not require solvents or water and can be operated at atmospheric pressure and room temperature. The extraction results were identified by gas chromatography–mass spectrometry (GC-MS). It was found that d-limonene was the most abundant compound in the extracted essential oils, comprising 95% of the total, with an additional 17 compounds detected. For 100 grams of pomelo peel, the energy requirement was 336.0 kJ to achieve the highest yield of 1.2% essential oil by weight of the pomelo peel plant material. The essential oil showed the antioxidant activities of IC50 at 4.87 + 0.06 mg/mL using ABTS radical scavenging assay.

Downloads

Download data is not yet available.

Article Details

How to Cite
Chutvirasakul, B., Riangnam, T., Kunkamphon, W., & Ramakul, P. (2025). Extraction of d-limonene from pomelo (Citrus maxima) peel waste using solvent-free microwave process and antioxidant activity analysis. Science, Engineering and Health Studies, 19, 25040008. https://doi.org/10.69598/sehs.19.25040008
Section
Engineering

References

Akhavan-Mahdavi, S., Sadeghi, R., Esfanjani, A. F., Hedayati, S., Shaddel, R., Dima, C., Malekjani, N., Boostani, S., & Jafari, S. M. (2022). Nanodelivery systems for d-limonene; techniques and applications. Food Chemistry, 384, Article 132479. https://doi.org/10.1016/j.foodchem.2022.132479

Attard, T. M., Watterson, B., Budarin, V. L., Clarka, J. H., & Hunt, A. J. (2014). Microwave assisted extraction as an important technology for valorising orange waste. New Journal of Chemistry, 38(6), 2278–2283. https://doi.org/10.1039/C4NJ00043A

d’ Alessio, P. A., Béné, M. C., & Menut, C. (2022). d-Limonene challenging anti-inflammatory strategies, AIMS Molecular Science, 9(2), 46–65. https://doi.org/10.3934/molsci.2022003

Eddin, L. B., Jha, N. K., Meeran, M. F. N., Kesari, K. K., Beiram, R., & Ojha, S. (2021). Neuroprotective potential of limonene and limonene containing natural products. Molecules, 26(15), Article 4535. https://doi.org/10.3390/molecules26154535

Felicia, W. X. L., Rovina, K., Aqilah, N. M. N., & Jaziri, A. A. (2024). Optimisation of supercritical fluid extraction of orange (Citrus sinenis L.) peel essential oil and its physicochemical properties. Current Research in Green and Sustainable Chemistry, 8, Article 100410. https://doi.org/10.1016/j.crgsc.2024.100410

Ferhat, M. A., Meklati, B. Y., Smadja, J., & Chemat, F. (2006). An improved microwave Clevenger apparatus for distillation of essential oils from orange peel. Journal of Chromatography A, 1112(1–2), 121–126. https://doi.org/10.1016/j.chroma.2005.12.030

Ghadiri, K., Raofie, F., Qomi, M., & Davoodi, A. (2020). Response surface methodology for optimization of supercritical fluid extraction of orange peel essential oil. Pharmaceutical and Biomedical Research, 6(4), 303–312. https://doi.org/10.18502/pbr.v6i4.5117

Kusuma, H., Putri, D., Dewi, I., & Mahfud, M. (2016). Solvent-free microwave extraction as the useful tool for extraction of edible essential oils. Chemistry & Chemical Technology, 10(2), 213–218. https://doi.org/10.23939/chcht10.02.213

Kusuma, H. S., & Mahfud, M. (2015). Preliminary study: Kinetics of oil extraction from sandalwood by microwave-assisted hydrodistillation. ASEAN Journal of Chemical Engineering, 15(2), 62–69. https://doi.org/10.22146/ajche.49687

Kusuma, H. S., Syahputra, M. E., Parasandi, D., Altway, A., & Mahfud, M. (2017). Optimization of microwave hydrodistillation of dried patchouli leaves by response surface methodology. Rasāyan Journal of Chemistry, 10(3), 861–865. https://doi.org/10.7324/RJC.2017.1031763

Ni, Z.-J., Wang, X., Shen, Y., Thakur, K., Han, J., Zhang, J.-G., Hu, F., & Wei, Z.-J. (2021). Recent updates on the chemistry, bioactivities, mode of action, and industrial applications of plant essential oils. Trends in Food Science & Technology, 110, 78–89. https://doi.org/10.1016/j.tifs.2021.01.070

Putri, D. K. Y., Dewi, I. E. P., Kusuma, H. S., & Mahfud, M. (2019). Extraction of an essential oil from fresh cananga flowers (Cananga odorata) using solvent-free microwave method. Journal of Chemical Technology and Metallurgy, 54(4), 793–802.

Vieira, A. J., Beserra, F. P., Souza, M. C., Totti, B. M., & Rozza, A. L. (2018). Limonene: Aroma of innovation in health and disease. Chemico-Biological Interactions, 283, 97–106. https://doi.org/10.1016/j.cbi.2018.02.007

Yang, J., & Park, M.-J. (2025). Antioxidant effects of essential oils from the peels of citrus cultivars. Molecules, 30(4), Article 833. https://doi.org/10.3390/molecules30040833