Somatic Embryogenesis Induction from Protocorm-like Bodies and Leaf Segments of Dendrobium Sonia ‘Earsakul’
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Published:
Sep 1, 2015
Keywords:
Dendrobium plant growth regulators (PGRs) protocorm-like bodies (PLBs) somatic embryogenesis
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Abstract
This study aimed to evaluate effects of types and concentrations of plant growth regulators in a halfstrength Murashige and Skoog medium on somatic embryogenesis from protocorm-like bodies (PLBs) and leaf segments of Dendrobium Sonia ‘Earsakul’ cultured for 8 weeks. It was found that media containing thidiazuron (TDZ) either at 0.1, 0.3 and 1.0 mg/L alone or in combinations with 0.1 mg/L naphthaleneacetic acid (NAA) or 1.0 mg/L 2,4 dichlorophenoxyacetic acid (2,4-D) provided no significant differences in percentage of somatic embryogenesis (38.18-49%) from PLBs. Half strength MS supplemented with TDZ either at 1 and 3 mg/L alone or combination with 0.1 mg/L NAA provided higher percentage of somatic embryo formation and number of somatic embryo per explant more than supplemented with 0.1 mg/L NAA alone. Low percentage of somatic embryogenesis was found from leaf segments. Leaf explants with the dorsal surface in contact with a medium provided higher somatic embryogenesis (0-18.75%) than those obtained from the explants with the ventral surface contact with culture medium (0.89 8.04%). Furthermore, basal segments of leaves with ventral surface in contact with a medium showed higher somatic embryogenesis and number of embryos (1.79-16.07% and 0.2-0.85, respectively) than those obtained from apical segments (0-1.78% and 0-0.3, respectively). There was a significant difference in somatic embryogenesis (0-18.75%) of a leaf when a dorsal surface was placed on the culture medium.
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Juntada, K., Taboonmee, S., Meetum, P., Poomjae, S., & Na Chiangmai, P. (2015). Somatic Embryogenesis Induction from Protocorm-like Bodies and Leaf Segments of Dendrobium Sonia ‘Earsakul’. Science, Engineering and Health Studies, 9(2), 9–19. Retrieved from https://li01.tci-thaijo.org/index.php/sehs/article/view/39201
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Research Articles
References
Alam, M. K., Rashid, M. H., Hossain, M. S., Salam, M. A., and Rauf, M. A. (2002). In vitro seed propagation of Dendrobium (Dendrobium transparens) orchid as influenced by different media. Biotechnology, 1: 111-115.
Anjum, S., Zia, M., and Chaudhary, M. F. (2006). Investigations of different strategies for high frequency regeneration of Dendrobium malones ‘Victory’. African Journal of Biotechnology, 5(19): 1738-1743.
Antony, J. J. J., Keng, C. L., Rathinam, X., Sinniah, U. R., and Subramaniam, S. (2010). Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm-like bodies by vitrification African Journal of Biotechnology, 9(42): 7063-7070.
Atichart, P. (2013). Polyploid induction by colchicine treatments and plant regeneration of Dendrobium chrysotoxum. Thai Journal of Agricultural Science, 46(1): 59-63.
Bairu, M. W., Fennell, C. W., and van Staden, J. (2006). The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Scientia Horticulurae, 108(4): 347-351.
Chang, C. and Chang, W. C. (1998). Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Report, 17: 251-255.
Chen, J. T., Chang, C., and Chang, W. C. (1999). Direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration. Plant Cell Report, 19: 143-149.
Chen, J. T. and Chang, W. C. (2000a). Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae). Plant Science, 160: 87-93.
Chen, J. T. and Chang, W. C. (2000b). Plant regeneration via embryo and shoot bud formation from lower-stalk explants of Oncidium Sweet Sugar. Plant Cell Tissue and Organ Culture, 62: 95-100.
Chen, J. T. and Chang, W. C. (2001). Effects of auxins and cytokinins on direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey’. Plant Growth Regulation, 34: 229-232.
Chia, T. F., Lim, Y. H., Luan, Y., and Ng, I. (2001). Transgenic Dendrobium (Orchid). Transgenic Crop III. Biotechnology in Agriculture and Forestry, 48: 95-106.
Chung, H. H., Chen, J. T., and Chang, W. C. (2005). Cytokinins induce direct somatic embryogenesis of Dendrobium Chiengmai Pink and subsequent plant regeneration. In Vitro Cellular & Developmental Biology – Plant, 41: 765-769.
Chung, H. H., Chen, J. T., and Chang, W. C. (2007). Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biologia Plantarum, 51(2): 346-350.
Dunlap, J. R., Kresovich, S., and McGee, R. E. (1986). The effect of salt concentration on auxin stability in culture media. Plant Physiology, 81: 934-936.
Ernst, R. (1994). Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae). Plant Cell, Tissue and Organ Culture, 39: 273-275.
Gong, Y. Q., Fan, Y., Wu, D. Z., Yang, H., Hu, Z. B., and Wang, Z. T. (2004). In vivo and in vitro evaluation of erianin, a novel antiangiogenic agent. European Journal of Cancer, 40: 1554-1565.
Huan, L. V. T., Takamura, T., and Tanaka, M. (2004). Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid. Plant Science, 166: 1443-1449.
Huetteman, C. A. and Preece, J. E. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell, Tissue and Organ Culture, 33:105-119.
Information Center, Deparment of Agricultural Extension. (2007). Agricultural Statistics. [Online URL: www.agriinfo.doae.go.th] accessed on May 5, 2014.
Jitsopakul, N. (2008). Vitrification-based cryopreservation of Vanda coerulea Griff. ex Linal. PH.D Dissertation. Faculty of Graduate Studies. Mahidol University.
Jones, W. E., Kuehnle, A. R., and Arumuganathan, K. (1998). Nuclear DNA content of 26 orchids (Orchidaceae) genera with emphasis on Dendrobium. Annals of Botany, 82: 189-184.
Kasikorn Research Center. (2006). Cut orchids. Business Brief 1846. [Online URL:www. kasikornresearch.com/EN/K-EconAnalysis/Pages/ViewSummary] accessed on May 5, 2014.
Kaewduangta, W. and Reamkatog, P. (2011). Effect of modification medium on growth development of Dendrobium parishii in vitro. American- Eurasian Journal of Agricultural & Environmental Sciences, 11(1): 117-121.
Khentry, Y., Paradornuvat, A., Tantiwiwat, S., Phansiri, S., and Thaveechai, N. (2006). Protoplast isolation and culture of Dendrobium Sonia “Bom 17”. Kasetsart Journal (Natural Science), 40: 361-369.
Khentry, Y., Chowpongpang, S., Paradormuwat, A., Tantiwiwat, S., and Thaveechai, N. (2007). Detection of Cymbidium mosaic virus in protocorm-like bodies in Dendrobium Sonia using one-step RT-PCR. Journal of Plant Biochemistry and Biotechnology, 16(2): 123-125.
Khosravi, A. R., Kadir, M. A., Kazemin, S. B., Zaman, F. Q., and De Silva, A. E. (2008). Establishment of a plant regeneration system from callus of Dendrobium cv. Serdang Beauty. African Journal of Biotechnology, 7(22): 4093-4099.
Kunasakdakul, K. and Smitamana, P. (2003). Dendrobium Pratum Red protoplast. Thai Journal of Agricultural Science, 36(1): 1-8.
Kuo, H. L., Chen, J. T., and Chang, W. C. (2005). Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis ‘Little Steve’. In Vitro Cellular Development Biology-Plant, 41: 453-456.
Lim, W. L. and Loh, C. S. (2003). Endopolyploidy in Vanda Miss Joaquim (Orchidaceae). New Phytologist, 159: 279-287.
Liu, R. J., Meng, A. D., Deng, X. Q., and Li, Y. R. (1988). Seedling rapid propagation of Dendrobium candidum. Acta Pharmaceutica, 23: 636-640.
Mahendran, G. and Narmatha Bai, V. (2012). Direct somatic embryogenesis and plant regeneration from seed derived protocorms of Cymbidium bicolor Lindl. Scientia Horticulturae, 135: 40-44.
Malabadi, R. B. and Nataraja, K. (2006). Cryopreservation and plant regeneration via somatic embryogenesis using shoot apical domes of mature Pinus roxburghii Sarg. Trees. In Vitro Cellular & Developmental Biology – Plant, 42: 152-159.
Martin, K. P., Geevarghese, J., Joseph, D., and Madassery, J. (2005). In vitro propagation of Dendrobium hybrids using flower stalk node explant. Indian Journal of Experimental Biology, 43: 280-285.
Mei, T. A., Danial, M., Mahmood, M., and Subramaniam, S. (2012). Exquisite protocol of callus induction and protocorm-like bodies (PLBs) regeneration of Dendrobium Sonia-28. Australian Journal of Crop Science, 6(5): 793-800.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15: 473-497.
Nayak, N. R., Sahoo, S., Patnaik, S., and Rath, S. P. (2002). Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. (Orchidaceae). Scientia Horticulturae, 94: 107-116.
Nehra, N. S., Kartha, K. K., Stushnoff, C., and Giles, K. L. (1992). The influence of plant growth regulator concentrations and callus age on somaclonal variation in callus culture regenerants of strawberry. Plant Cell, Tissue and Organ Culture, 29: 257-268.
Nissen, S. J. and Sutter, E. G. (1990). Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience, 25(7): 800-802.
Pinthong, R., Sujipuli, K., and Ratanasut, K. (2014). Agroinfiltration for transient gene expression in floral tissues of Dendrobium Sonia ‘Earsakul’. Journal of Agricultural Technology, 10(2): 459-465.
Pontaroli, A. C. and Camadro, E. L. (2005). Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long- term callus cultures. Genetics and Molecular Biology, 28(3): 423-430.
Pradhan, U. C. (1979). Indian orchids: guide to identification and culture, Vol II. Published by UC Pradhan, Kalimpong, India.
Roy, J., Naha, S., Majumdar, M., and Banerjee, N. (2007). Direct and callus-mediated protocorm- like body induction from shoot-apicals of Dendrobium chrysotoxum Lindl. (Orchidaceae). Plant Cell, Tissue and Organ Culture, 90: 31-39.
Shiau, Y. J., Nalawade, S. M., Hsia, C. N., Mulabagal, V., and Tsay, H. S. (2005). In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. ex Lindl., from axenic nodal segments. In Vitro Cellular and Developmental Biology-Plant, 41: 666-670.
Sujjaritthurakarn, P. and Kanchanapoom, K. (2011). Efficient direct protocorm-like bodies induction of dwarf Dendrobium using Thidiazuron. Notulae Scientia Biologicae, 3(4): 88-92.
Sunitibala, H. and Kishor, R. (2009). Micropropagation of Dendrobium transparens L. from axenic pseudobulb segments. Indian Journal of Biotechnology, 8: 448-452.
Suzuki, M., Tandon, P., Ishikawa, M., and Toyomasu, T. (2008). Development of a new vitrification solution, VSL and its application to the cryopreservation of gentian axillary buds. Plant Biotechnology, 2: 123-131.
Temjensangba and Deb, C. R. (2005). Regeneration of plantlets from in vitro raised leaf explants of Cleisostoma racimeferum Lindl. Indian Journal of Experimental Biology, 43: 377-381.
Tokuhara, K. and Mii, M. (2003). Highly-efficient somatic embryogenesis from cell suspension cultures of Phalaenopsis orchids by adjusting carbohydrate sources. In Vitro Cellular and Developmental Biology-Plant, 39(6): 635-639.
Vacin, E. and Went, F. (1949). Some pH changes in nutrient solution. Botanic Gardens Conservation News, 110: 605-613.
Zhang, Z. G., Wang, L., Liu, H., Cai, Z. G., Han, X. Z., Zhao, L. H., and Luo, Z. J. (1993). Studies on culture medium for protocorm differentiation medium of Dendrobium candidum Wall. ex Lindl. China Journal of Chinese Materia Medica, 18: 16-19.
Zhao, P., Wu, F., Feng, F. S., and Wang, W. J. (2008). Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl. In Vitro Cellular and Developmental Biology-Plant, 44(3): 178-185.
Anjum, S., Zia, M., and Chaudhary, M. F. (2006). Investigations of different strategies for high frequency regeneration of Dendrobium malones ‘Victory’. African Journal of Biotechnology, 5(19): 1738-1743.
Antony, J. J. J., Keng, C. L., Rathinam, X., Sinniah, U. R., and Subramaniam, S. (2010). Preliminary study on cryopreservation of Dendrobium Bobby Messina protocorm-like bodies by vitrification African Journal of Biotechnology, 9(42): 7063-7070.
Atichart, P. (2013). Polyploid induction by colchicine treatments and plant regeneration of Dendrobium chrysotoxum. Thai Journal of Agricultural Science, 46(1): 59-63.
Bairu, M. W., Fennell, C. W., and van Staden, J. (2006). The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Scientia Horticulurae, 108(4): 347-351.
Chang, C. and Chang, W. C. (1998). Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Report, 17: 251-255.
Chen, J. T., Chang, C., and Chang, W. C. (1999). Direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration. Plant Cell Report, 19: 143-149.
Chen, J. T. and Chang, W. C. (2000a). Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae). Plant Science, 160: 87-93.
Chen, J. T. and Chang, W. C. (2000b). Plant regeneration via embryo and shoot bud formation from lower-stalk explants of Oncidium Sweet Sugar. Plant Cell Tissue and Organ Culture, 62: 95-100.
Chen, J. T. and Chang, W. C. (2001). Effects of auxins and cytokinins on direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey’. Plant Growth Regulation, 34: 229-232.
Chia, T. F., Lim, Y. H., Luan, Y., and Ng, I. (2001). Transgenic Dendrobium (Orchid). Transgenic Crop III. Biotechnology in Agriculture and Forestry, 48: 95-106.
Chung, H. H., Chen, J. T., and Chang, W. C. (2005). Cytokinins induce direct somatic embryogenesis of Dendrobium Chiengmai Pink and subsequent plant regeneration. In Vitro Cellular & Developmental Biology – Plant, 41: 765-769.
Chung, H. H., Chen, J. T., and Chang, W. C. (2007). Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biologia Plantarum, 51(2): 346-350.
Dunlap, J. R., Kresovich, S., and McGee, R. E. (1986). The effect of salt concentration on auxin stability in culture media. Plant Physiology, 81: 934-936.
Ernst, R. (1994). Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae). Plant Cell, Tissue and Organ Culture, 39: 273-275.
Gong, Y. Q., Fan, Y., Wu, D. Z., Yang, H., Hu, Z. B., and Wang, Z. T. (2004). In vivo and in vitro evaluation of erianin, a novel antiangiogenic agent. European Journal of Cancer, 40: 1554-1565.
Huan, L. V. T., Takamura, T., and Tanaka, M. (2004). Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid. Plant Science, 166: 1443-1449.
Huetteman, C. A. and Preece, J. E. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell, Tissue and Organ Culture, 33:105-119.
Information Center, Deparment of Agricultural Extension. (2007). Agricultural Statistics. [Online URL: www.agriinfo.doae.go.th] accessed on May 5, 2014.
Jitsopakul, N. (2008). Vitrification-based cryopreservation of Vanda coerulea Griff. ex Linal. PH.D Dissertation. Faculty of Graduate Studies. Mahidol University.
Jones, W. E., Kuehnle, A. R., and Arumuganathan, K. (1998). Nuclear DNA content of 26 orchids (Orchidaceae) genera with emphasis on Dendrobium. Annals of Botany, 82: 189-184.
Kasikorn Research Center. (2006). Cut orchids. Business Brief 1846. [Online URL:www. kasikornresearch.com/EN/K-EconAnalysis/Pages/ViewSummary] accessed on May 5, 2014.
Kaewduangta, W. and Reamkatog, P. (2011). Effect of modification medium on growth development of Dendrobium parishii in vitro. American- Eurasian Journal of Agricultural & Environmental Sciences, 11(1): 117-121.
Khentry, Y., Paradornuvat, A., Tantiwiwat, S., Phansiri, S., and Thaveechai, N. (2006). Protoplast isolation and culture of Dendrobium Sonia “Bom 17”. Kasetsart Journal (Natural Science), 40: 361-369.
Khentry, Y., Chowpongpang, S., Paradormuwat, A., Tantiwiwat, S., and Thaveechai, N. (2007). Detection of Cymbidium mosaic virus in protocorm-like bodies in Dendrobium Sonia using one-step RT-PCR. Journal of Plant Biochemistry and Biotechnology, 16(2): 123-125.
Khosravi, A. R., Kadir, M. A., Kazemin, S. B., Zaman, F. Q., and De Silva, A. E. (2008). Establishment of a plant regeneration system from callus of Dendrobium cv. Serdang Beauty. African Journal of Biotechnology, 7(22): 4093-4099.
Kunasakdakul, K. and Smitamana, P. (2003). Dendrobium Pratum Red protoplast. Thai Journal of Agricultural Science, 36(1): 1-8.
Kuo, H. L., Chen, J. T., and Chang, W. C. (2005). Efficient plant regeneration through direct somatic embryogenesis from leaf explants of Phalaenopsis ‘Little Steve’. In Vitro Cellular Development Biology-Plant, 41: 453-456.
Lim, W. L. and Loh, C. S. (2003). Endopolyploidy in Vanda Miss Joaquim (Orchidaceae). New Phytologist, 159: 279-287.
Liu, R. J., Meng, A. D., Deng, X. Q., and Li, Y. R. (1988). Seedling rapid propagation of Dendrobium candidum. Acta Pharmaceutica, 23: 636-640.
Mahendran, G. and Narmatha Bai, V. (2012). Direct somatic embryogenesis and plant regeneration from seed derived protocorms of Cymbidium bicolor Lindl. Scientia Horticulturae, 135: 40-44.
Malabadi, R. B. and Nataraja, K. (2006). Cryopreservation and plant regeneration via somatic embryogenesis using shoot apical domes of mature Pinus roxburghii Sarg. Trees. In Vitro Cellular & Developmental Biology – Plant, 42: 152-159.
Martin, K. P., Geevarghese, J., Joseph, D., and Madassery, J. (2005). In vitro propagation of Dendrobium hybrids using flower stalk node explant. Indian Journal of Experimental Biology, 43: 280-285.
Mei, T. A., Danial, M., Mahmood, M., and Subramaniam, S. (2012). Exquisite protocol of callus induction and protocorm-like bodies (PLBs) regeneration of Dendrobium Sonia-28. Australian Journal of Crop Science, 6(5): 793-800.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15: 473-497.
Nayak, N. R., Sahoo, S., Patnaik, S., and Rath, S. P. (2002). Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. (Orchidaceae). Scientia Horticulturae, 94: 107-116.
Nehra, N. S., Kartha, K. K., Stushnoff, C., and Giles, K. L. (1992). The influence of plant growth regulator concentrations and callus age on somaclonal variation in callus culture regenerants of strawberry. Plant Cell, Tissue and Organ Culture, 29: 257-268.
Nissen, S. J. and Sutter, E. G. (1990). Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience, 25(7): 800-802.
Pinthong, R., Sujipuli, K., and Ratanasut, K. (2014). Agroinfiltration for transient gene expression in floral tissues of Dendrobium Sonia ‘Earsakul’. Journal of Agricultural Technology, 10(2): 459-465.
Pontaroli, A. C. and Camadro, E. L. (2005). Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long- term callus cultures. Genetics and Molecular Biology, 28(3): 423-430.
Pradhan, U. C. (1979). Indian orchids: guide to identification and culture, Vol II. Published by UC Pradhan, Kalimpong, India.
Roy, J., Naha, S., Majumdar, M., and Banerjee, N. (2007). Direct and callus-mediated protocorm- like body induction from shoot-apicals of Dendrobium chrysotoxum Lindl. (Orchidaceae). Plant Cell, Tissue and Organ Culture, 90: 31-39.
Shiau, Y. J., Nalawade, S. M., Hsia, C. N., Mulabagal, V., and Tsay, H. S. (2005). In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. ex Lindl., from axenic nodal segments. In Vitro Cellular and Developmental Biology-Plant, 41: 666-670.
Sujjaritthurakarn, P. and Kanchanapoom, K. (2011). Efficient direct protocorm-like bodies induction of dwarf Dendrobium using Thidiazuron. Notulae Scientia Biologicae, 3(4): 88-92.
Sunitibala, H. and Kishor, R. (2009). Micropropagation of Dendrobium transparens L. from axenic pseudobulb segments. Indian Journal of Biotechnology, 8: 448-452.
Suzuki, M., Tandon, P., Ishikawa, M., and Toyomasu, T. (2008). Development of a new vitrification solution, VSL and its application to the cryopreservation of gentian axillary buds. Plant Biotechnology, 2: 123-131.
Temjensangba and Deb, C. R. (2005). Regeneration of plantlets from in vitro raised leaf explants of Cleisostoma racimeferum Lindl. Indian Journal of Experimental Biology, 43: 377-381.
Tokuhara, K. and Mii, M. (2003). Highly-efficient somatic embryogenesis from cell suspension cultures of Phalaenopsis orchids by adjusting carbohydrate sources. In Vitro Cellular and Developmental Biology-Plant, 39(6): 635-639.
Vacin, E. and Went, F. (1949). Some pH changes in nutrient solution. Botanic Gardens Conservation News, 110: 605-613.
Zhang, Z. G., Wang, L., Liu, H., Cai, Z. G., Han, X. Z., Zhao, L. H., and Luo, Z. J. (1993). Studies on culture medium for protocorm differentiation medium of Dendrobium candidum Wall. ex Lindl. China Journal of Chinese Materia Medica, 18: 16-19.
Zhao, P., Wu, F., Feng, F. S., and Wang, W. J. (2008). Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl. In Vitro Cellular and Developmental Biology-Plant, 44(3): 178-185.