Absorption of Nucleic Acid from the Small Intestines of Swamp Buffaloes and Zebu Cattle
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swamp buffaloes Kedah Kelantan (KK) zebu cattle purine absorption nucleic acid
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Abstract
Three male yearling swamp buffaloes and three Kedah Kelantan (KK) zebu cattle of similar age and sex were used. Animals were each fed 4 kg of a total mixed ration diet (TMR) consisting of 40% oil palm frond and 60% concentrate for 2 wk. Cr2O3 was used as the indigestibility marker. On day 15, animals were sacrificed and the digesta in the small intestine was collected to study the absorption of purines in the segments, each being 60 cm in length of the small intestine. Deoxyribonucleic acid (DNA) disappearance from the first to the 15th segment did not differ between buffaloes and cattle (P > 0.05), averaging 94% and 91%, respectively. Ribonucleic acid (RNA) disappearance for buffaloes was lower than that for cattle (P < 0.05) (82% and 95%, respectively) but did not differ between species (P > 0.05). Similarly, purine absorption did not differ between buffaloes and cattle (P > 0.05). Total purine absorbed within 20 segments was 81% and 92% respectively for buffaloes and cattle. Although disappearance of RNA, DNA and the purine bases (PB) in the small intestine did not differ between cattle and swamp buffaloes (P > 0.05), based on concentration per Cr marker, the quantity of unabsorbed PB in the last 5 segments of the small intestine of buffaloes was higher than that of cattle (P < 0.05). Although this suggests that absorption of PB in the small intestine of buffaloes was lower than of cattle, it can not explain the large differences in the PD urinary excretion rates between the species.
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Pimpa, O., Liang, J. B., & Balcells, J. Q. (2013). Absorption of Nucleic Acid from the Small Intestines of Swamp Buffaloes and Zebu Cattle. Science, Engineering and Health Studies, 1(1), 26–35. Retrieved from https://li01.tci-thaijo.org/index.php/sehs/article/view/7110
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References
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Liang, J. B., Matsumoto, M. and Young, B. A. (1994). Purine derivative excretion and ruminal microbial yield in Malaysian cattle and swamp buffalo. Animal Feed Science and Technology, 47: 189-199.
Martin Orue, S. M., Balcells, J., Guada, J. A., and Castrillo, C. (1995). Endogenous purine and pyrimidine derivative excretion in pregnant sows. British Journal of Nutrition, 73: 375- 385.
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Perez, J. F., Balcells, J., Guada, J. A. and Castrillo, C. (1997). Contribution of dietary nitrogen and purine bases to duodenal digesta: comparison of duodenal and polyester-bag measurements. British Society of Animal Science, 65: 237-245.
Pimpa, O. (2002). Urinary purine derivatives excretion as a method for estimation of rumen microbial protein production in swamp buffaloes and Zebu cattle. PhD Thesis, Universiti Putra Malaysia.
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Smith, R. H. and McAllan, A. B. (1970). Nucleic acid metabolism in the ruminant. 2. Formation of microbial nucleic acids in the rumen in relation to the digestion of food nitrogen and the fate of dietary nucleic acids. British Journal of Nutrition, 24: 545-556.
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Smith, R. H., McAllan, A. B., and Hill, W. B. (1969). Nucleic acids in bovine nutrition. 3. Fate of nucleic acid presented to the small intestine. Proceeding of the Nutrition Society 28, 28A.
Vagnoni, D. B., Broderick, G. A., Clayton, M. K., and Hatfield, R. D. (1997). Excretion of purine derivatives by Holstein cows abomasally infused with incremental amounts of purines. Journal of Dairy Science, 80: 1695-1702.
Verbic, J., Chen, X. B., MacLeod, N. A., and Ørskov, E. R. (1990). Excretion of purine derivatives by ruminants: effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science (Cambridge), 114: 243-248.
Vercoe, J. E. (1976). Urinary allantoin excretion and digestible dry-matter intake in cattle and buffalo. Journal of Agricultural Science (Cambridge), 86: 613-615.
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. (1991). Urinary excretion of allantoin and allantoin precursors by sheep after different rate of purine infusion into the duodenum. Journal of Agricultural Science (Cambridge), 116: 309-317.
Balcells, J., Guada, J. A., Peiro, J. M. and Parker, D. S. (1992a). Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. Journal of Chromatography, 575: 153-157.
Balcells, J., Parker, D. S. and Seal, C. J. (1992b). Purine metabolite concentrations in portal and peripheral blood of steers, sheep and rats. Comparative Biochemistry and Physiology 101B, 4: 633-636.
Chen, X. B., Hovell, F. D. DeB., and Orskov, E. R. (1990a). Excretion of purine derivatives by ruminants: recycling of allantoin into the rumen via saliva and its fate in the gut. British Journal of Nutrition, 63: 197-205.
Chen., X. B., Hovell, F. D. DeB., Orskov, E.R. and Brown, D. S. (1990b). Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition, 63: 131-142.
Chen, X. B., Samaraweera, L., Kyle, D. J. and Orskov, E. R. (1996). Urinary excretion of purine derivatives and tissue xanthine oxidase (EC 1.2.3.2) activity in buffaloes (Bubalis bubalis) with special reference to difference between buffaloes and Bos taurus cattle. British. Journal of Nutrition, 75: 397-407.
Coehlo da Silva, J. F., Seeley, R. C., Beever, D. E., Prescott, J. H. D., and Armstrong, D. J. (1972). The effect in sheep of physical form and stage of growth on the site of digestion of a dried grass. 2. Sites of nitrogen digestion. British Journal of Nutrition, 28: 357.
Condon, R. J., Hall, G., and Hatfield, E. E. (1970). Metabolism of abomasally infused 14C-labelled RNA adenine, uracil and glycine. Journal of Animal Science, 31: 1037-1038 (abstr).
Christian, R. R., and Coup, M. R. (1954). Measurement of feed intake by grazing cattle and sheep. VI. The determination of chromic oxide in faeces. New Zeland Journal of Science and Technology. Sec. A., 36: 328-330.
Jackson, T. C., Schelling, G. T., Mitchell, G. E., and Tucker, R. E. (1976). Nucreic acid bases in sheep ingesta. Journal of Animal Science, 43: 325-326.
Liang, J. B., Matsumoto, M. and Young, B. A. (1994). Purine derivative excretion and ruminal microbial yield in Malaysian cattle and swamp buffalo. Animal Feed Science and Technology, 47: 189-199.
Martin Orue, S. M., Balcells, J., Guada, J. A., and Castrillo, C. (1995). Endogenous purine and pyrimidine derivative excretion in pregnant sows. British Journal of Nutrition, 73: 375- 385.
McAllan, A. B. (1980). The degradation of nucleic acid in, and the removal of breakdown products from the small intestines of steers. British Journal of Nutrition, 44: 99-112.
Molecular research center (MRC), Inc. (2000). Manufacturer's protocol 1995, TRI Reagent LS"DNA, RNA and protein from liquid samples, single step method. MRC. Inc. Cincinnati, Ohio, USA.
Perez, J. F., Balcells, J., Guada, J. A. and Castrillo, C. (1997). Contribution of dietary nitrogen and purine bases to duodenal digesta: comparison of duodenal and polyester-bag measurements. British Society of Animal Science, 65: 237-245.
Pimpa, O. (2002). Urinary purine derivatives excretion as a method for estimation of rumen microbial protein production in swamp buffaloes and Zebu cattle. PhD Thesis, Universiti Putra Malaysia.
Statistical Analysis Systems Institute Institute. (1988). SAS User's Guide statistic, Version 6.03, Cary, NC.
Siddons, R. C., Paradine, J., Beever, D. E. and Cornell, P.R. (1985). Ytterbium acetate as a particulate-phase digesta-flow marker. British Journal of Nutrition, 54: 509-519.
Smith, R. H. and McAllan, A. B. (1970). Nucleic acid metabolism in the ruminant. 2. Formation of microbial nucleic acids in the rumen in relation to the digestion of food nitrogen and the fate of dietary nucleic acids. British Journal of Nutrition, 24: 545-556.
Smith, R. H. and McAllan, A. B. (1971). Nucleic acid metabolism in the ruminant 3. Amounts of nucleic acids and total ammonia nitrogen in digesta from the rumen, duodenum and ileum of calves. British Journal of Nutrition, 25: 181-190.
Smith, R. H., McAllan, A. B., and Hill, W. B. (1969). Nucleic acids in bovine nutrition. 3. Fate of nucleic acid presented to the small intestine. Proceeding of the Nutrition Society 28, 28A.
Vagnoni, D. B., Broderick, G. A., Clayton, M. K., and Hatfield, R. D. (1997). Excretion of purine derivatives by Holstein cows abomasally infused with incremental amounts of purines. Journal of Dairy Science, 80: 1695-1702.
Verbic, J., Chen, X. B., MacLeod, N. A., and Ørskov, E. R. (1990). Excretion of purine derivatives by ruminants: effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science (Cambridge), 114: 243-248.
Vercoe, J. E. (1976). Urinary allantoin excretion and digestible dry-matter intake in cattle and buffalo. Journal of Agricultural Science (Cambridge), 86: 613-615.