Improvement of Drought Tolerance in Shorea roxburghii and Shorea siamensis Seedlings with Astraeus odoratus as Ectomycorrhiza

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Parichat Saengngam
Chanthipha Sridawong
Sulfa Yeerahem
Oraya Burakai
Jetsada Wongprom
Nisa Leksungnoen
Tharnrat Kaewgrajang


The aim of this study was to assess the drought tolerance of Shorea roxburghii and Shorea siamensis seedlings resulting from Astraeus odoratus acting as an ectomycorrhiza. The spore suspension of A. odoratus was inoculated into the two seedling species at a concentration of 10 ml/seedling. After one year, the drought tolerance of seedlings was determined by stopping irrigation for 7 weeks. During the drought tolerance experiment, all data were collected at four different times. The obtained data was then analyzed using t-test to compare the drought resistance between the inoculated and non-inoculated seedlings.

Results indicated that all the growth parameters of inoculated seedlings of S. roxburghii were not significantly higher than the non-inoculated seedlings when under saturated soil water content or 61-100 percent of soil moisture. When the soil moisture was reduced to between 41-60 percent, the inoculated S. roxburghii seedlings had a significantly higher performance index, Fv/Fm, and leaf area than those of the non-inoculated seedlings. For the S. siamensis seedlings, it was found that the inoculated seedlings had a significantly higher diameter at root collar, height, and leaf surface area compared to those of the non-inoculated seedlings under saturated soil water content. However, the inoculated S. siamensis seedlings had a significantly higher diameter at root collar than the non-inoculated control when the soil moisture was reduced to between 21-40 percent. Our results indicate that inoculation of A. odoratus on both the seedling species can improve drought tolerance of the two dipterocarp seedlings.


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Saengngam, P. ., Sridawong, C., Yeerahem, S., Burakai, O., Wongprom, J., Leksungnoen, N., & Kaewgrajang, T. (2021). Improvement of Drought Tolerance in Shorea roxburghii and Shorea siamensis Seedlings with Astraeus odoratus as Ectomycorrhiza. Thai Journal of Forestry, 40(2), 69–82. Retrieved from
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