Effect of Planting Material and Spore Preservation Temperature of Astraeus odoratus on the Growth and Ectomycorrhizal Root Formation in Dipterocarpus alatus seedlings
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Abstract
The objectives of this study were to study the effect of planting materials and temperature on the preservation of Astraeus odoratus spores and their ability to grow and form ectomycorrhizal (ECM) root in Dipterocarpus alatus seedlings. Two formulae of planting materials included Formula 1 consisting of vermiculite + peat moss (8:2); and Formula 2 consisting of soil + granules black rice husk + coconut husk (1:1:1), were used in the experiment. The result indicated that seedlings inoculated with Formula 1 formed ECM colonization at a rate of 42.09%, which was not observed with Formula 2. Moreover, a relative growth rate of height was observed which was significantly higher than that in seedlings inoculated with Formula 2. To study the effect of temperature on preservation of A. odoratus spores, we preserved the spores for two years under three different temperatures including room temperature, 4 °C, and -20 °C. The result indicated that a 2-year-old preserved spore can be used as an ECM inoculum. The appropriate temperature for preservation spore was -20 °C. In addition, the inoculated seedlings with spores preserved at -20 °C showed a significantly higher ECM colonization rate (61.04%) than the inoculated seedlings with spores preserved at room temperature (22.51%).
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ข้าพเจ้าและผู้เขียนร่วม (ถ้ามี) ขอรับรองว่า ต้นฉบับที่เสนอมานี้ยังไม่เคยได้รับการตีพิมพ์และไม่ได้อยู่ในระหว่างกระบวนการพิจารณาตีพิมพ์ลงในวารสารหรือสิ่งตีพิมพ์อื่นใด ข้าพเจ้าและผู้เขียนร่วม (ถ้ามี) ยอมรับหลักเกณฑ์และเงื่อนไขการพิจารณาต้นฉบับ ทั้งยินยอมให้กองบรรณาธิการมีสิทธิ์พิจารณาและตรวจแก้ต้นฉบับได้ตามที่เห็นสมควร พร้อมนี้ขอมอบลิขสิทธิ์ผลงานที่ได้รับการตีพิมพ์ให้แก่วารสารวนศาสตร์ คณะวนศาสตร์ มหาวิทยาลัยเกษตรศาสตร์ กรณีมีการฟ้องร้องเรื่องการละเมิดลิขสิทธิ์เกี่ยวกับภาพ กราฟ ข้อความส่วนใดส่วนหนึ่ง หรือ ข้อคิดเห็นที่ปรากฏในผลงาน ให้เป็นความรับผิดชอบของข้าพเจ้าและผู้เขียนร่วม (ถ้ามี) แต่เพียงฝ่ายเดียว และหากข้าพเจ้าและผู้เขียนร่วม (ถ้ามี) ประสงค์ถอนบทความในระหว่างกระบวนการพิจารณาของทางวารสาร ข้าพเจ้าและผู้เขียนร่วม (ถ้ามี) ยินดีรับผิดชอบค่าใช้จ่ายทั้งหมดที่เกิดขึ้นในกระบวนการพิจารณาบทความนั้น”
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