Temporary Immersion System (TIS) for Micropropagation of Dendrocalamus latiflorus in Commercial Production

Abstract

Improvement of tissue culture techniques for shoots of Ma-chu Bamboo (Dendrocalamus latiflorus) were cultured in three different conditions in Murashige and Skoog (MS) medium supplemented with Benzylaminopurine (BAP) 4 mg 1-1, Naphthaleneacetic acid (NAA) and 6-furfurylaminopurin (kinetin) 1, 1 mg 1^-11 and coconut water 8 mg 1^-1. The conditions included liquid medium with temporary immersion system (TIS), plant cultures by intermittent immersion in medium with this simple system by pneumatic and time controllers, for 1 minute in every 1 or 3 hours (TIS₁), 5 minutes in every 3 hours (TIS₂) and semi-solid medium. The highest shoot multiplication number in 60 days were obtained from liquid medium with TIS₂, 105 shoots (multiplication rate of 7.75 X) compared to 83 shoots (multiplication rate of 5.92 X) in TIS₁ and 27 shoots (multiplication rate of 1.25 X) in semi-solid conditions. The TIS₂ gave higher shoot production than semi-solid culture medium about 6.5 folds in shoot multiplication rate and shoots were healthy and produced internodes elongation. Therefore, the TIS is a promising technique for reducing costs of Ma-chu bamboo plantlets in commercial production from tissue culture technique in the future.

Keywords: temporary immersion system (TIS), Dendrocalamus latiflorus, micropropagation