GENETIC DISCRIMINATION OF EUCALYPTUS CAMALDULENSIS DEHNH. BY DNA FINGERPRINTING

Abstract

Genetic discrimination of Eucalyptus camaldulensis Dehnh. was carried out by using random amplified polymorphic DNA (RAPD) fingerprint. There were 38 samples composed of clone S10, clone TS, 14 selfing progenies and 22 outcrossing progenies of both clones. The objectives of this study were to develope technique for genetic discrimination of E. camaldulensis, to discriminate DNA fingerprint among samples and to analyse their genetic relationship using the NTSYS - PC computer program version 1.8. It was found that the modified method of Agrawal et al. (1992) could be efficiently used for DNA extraction of E. camaldulensis. The total of 82 decamer primers were screened and 10 of them were found to give clear reproducible result. 51 bands were observed, only 6 primers showed polymorphism among samples and 5 primers could be used for genetic linkage map and marker assisted selection (MAS). The segregation of homozygous and heterozygous were undergoing to Mendel's Law. The genetic similarity of all studied samples were greater than 90 percent. DNA fingerprinting data revealed clear separation and could be divided into two groups. Both of them were significantly different in some specific bands of DNAs.