Genetic Relationship among Paphiopedilum Subgenus Brachypetalum Section Brachypetalum Using HAT-RAPD Markers
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Published:
Dec 4, 2017
Keywords:
Paphiopedilum genetic relationship HAT-RAPD marker
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Abstract
Abstract
Genetic relationship of lady’s slipper orchids, Paphiopedilum subgenus Brachypetalum section Brachypetalum, 14 varieties found in Thailand, was demonstrated by HAT-RAPD markers. The total of 72 random primers were screened and 29 primers could be used for DNA amplification. Twenty-two primers were selected and used to analyze each DNA species of all samples. A dendrogram was constructed, based on polymorphic bands, and showed genetic similarities among 4 clusters with the similarity coefficients ranging from 0.32 to 0.81 as well as the analysis of genetic diversity.
Keywords: Paphiopedilum; genetic relationship; HAT-RAPD marker
Article Details
How to Cite
Meesangiem, T., Damrianant, S., Thanananta, T., & Thanananta, N. (2017). Genetic Relationship among Paphiopedilum Subgenus Brachypetalum Section Brachypetalum Using HAT-RAPD Markers. Thai Journal of Science and Technology, 7(1), 99–105. https://doi.org/10.14456/tjst.2018.2
Section
วิทยาศาสตร์ชีวภาพ
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ข้อความที่ปรากฏในแต่ละเรื่องของวารสารเล่มนี้เป็นเพียงความเห็นส่วนตัวของผู้เขียน ไม่มีความเกี่ยวข้องกับคณะวิทยาศาสตร์และเทคโนโลยี หรือคณาจารย์ท่านอื่นในมหาวิทยาลัยธรรมศาสตร์ ผู้เขียนต้องยืนยันว่าความรับผิดชอบต่อทุกข้อความที่นำเสนอไว้ในบทความของตน หากมีข้อผิดพลาดหรือความไม่ถูกต้องใด ๆ
References
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Tansa-nga, W., Thanananta, T. and Thana-nanta, N., 2014, Identification and genetic relationship analysis of Aerides varieties using high annealing temperature-random amplified polymorphic DNA (HAT-RAPD) and inter-simple sequence repeat (ISSR) techniques, Thai J. Sci. Technol. 3: 102-112.
Thanananta, N., Prasit, V. and Thanananta, T., 2012, Identification of rice cultivars KDML105 and its improved cultivars by using HAT-RAPD technique, Thai J. Sci. Technol. 1: 169-179.
Thanananta, N., Pumeiam, S. and Thanananta, T., 2013, Identification and genetic relationship of Rhynchostylis gigantean and their hybrids using HAT-RAPD markers, Thai J. Sci. Technol. 4: 360-370.
Thomsopa, T., Thanananta, T. and Thanananta, N., 2014, Identification and genetic relationship analysis of Dendrobium spp., Ueang Sai group, using HAT-RAPD and ISSR techniques, Thai J. Sci. Technol. 3: 82-91.
Tongsom, J., Thanananta, T. and Thanananta, N., 2015, Genetic relationship among Vanda section Ascocentrum based on HAT-RAPD and ISSR, Thai J. Sci. Technol. 23: 475-484.
Wanichananan, P., 2000, A study on species relationship in the section Brachypetalum of Paphiopedilum using RAPD technique, Ph.D. Thesis, Kasetsart University, Bangkok.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.U., 1990, DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucl. Acids Res. 8: 6531-6535.
Zhang, F., Lv, Y., Dong, H. and Guo, S., 2010, Analysis of genetic stability through inter simple sequence repeats molecular markers in micro propagated plantlets of Anoectochilus formosanus Hayata, a medicinal plant, Biol. Pharm. Bull. 33: 384-388.
Cox, A.V., Pridgeon, A.M., Albert, V.A. and Chase, M.W., 1997, Phylogenetics of the slipper orchids (Cypripedioideae, Orchidaceae): Nuclear rDNA ITS sequences, Plant Syst. Evol. 208: 197-223.
Doyle, J.J. and Doyle, J.L., 1987, A rapid DNA isolation procedure for small quantities of fresh leaf tissue, Polym. Bull. 19: 11-15.
Guo, Y.Y, Huang, L.Q., Liu, Z.J. and Wang, X.Q., 2016, Promise and challenge of DNA barcoding in Venus slipper (Paphiopedilum), Plos ONE, 11(1): e0146880.
Lertnitikul, N., Jittham, P., Khankhampoch, L., Pattamadilok, C., Sukrong, S. and Suttisri, R., 2016, Cytotoxic stilbenes from the roots of Paphiopedilum godefroyae, J. Asian Nat. Prod. Res. 18: 1143-1150.
Naphatsawan, P., Nonthalert, L., Rutt, S. and Suchada, S., 2016, Stilbenes from Paphiopedilum exul roots, TJPS. 40: 116-119.
Netnaree, S., 2006, Orchids, 5th Ed, Kasetsiambook Publications, Bangkok.
Peyachoknagul, S., 2009, DNA marker, Kasetsart University Publications, Bangkok.
Pumikong, S., Vanichanon, A. and Upho, U., 2011, Morphological charecters and genetic diversity of Lady’s slipper orchids (Paphiopedilum thaianum, P. niveum and P. godefroyae) in some locations of southern Thailand, Faculty of Science, Thaksin University, Songkhla.
Rohlf, F.J., 2002, NTSYpc Numerical Taxonomy and Multivariate Analysis System, Applied Biostatistics, Inc., New York.
Saetai, K., Thanananta, T. and Thanananta, N., 2014, Identification and genetic relationship analysis of Bulbophyllum section Sestochilus using HAT-RAPD and ISSR markers, Thai J. Sci. Technol. 3: 92-101.
Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989, Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, New York.
Sumrith, J., Thanananta, T. and Thanananta, N., 2014, Identification and genetic relationship analysis of colored rice cultivars using HAT-RAPD and ISSR techniques, Thai J. Sci. Technol. 3: 113-122.
Tansa-nga, W., Thanananta, T. and Thana-nanta, N., 2014, Identification and genetic relationship analysis of Aerides varieties using high annealing temperature-random amplified polymorphic DNA (HAT-RAPD) and inter-simple sequence repeat (ISSR) techniques, Thai J. Sci. Technol. 3: 102-112.
Thanananta, N., Prasit, V. and Thanananta, T., 2012, Identification of rice cultivars KDML105 and its improved cultivars by using HAT-RAPD technique, Thai J. Sci. Technol. 1: 169-179.
Thanananta, N., Pumeiam, S. and Thanananta, T., 2013, Identification and genetic relationship of Rhynchostylis gigantean and their hybrids using HAT-RAPD markers, Thai J. Sci. Technol. 4: 360-370.
Thomsopa, T., Thanananta, T. and Thanananta, N., 2014, Identification and genetic relationship analysis of Dendrobium spp., Ueang Sai group, using HAT-RAPD and ISSR techniques, Thai J. Sci. Technol. 3: 82-91.
Tongsom, J., Thanananta, T. and Thanananta, N., 2015, Genetic relationship among Vanda section Ascocentrum based on HAT-RAPD and ISSR, Thai J. Sci. Technol. 23: 475-484.
Wanichananan, P., 2000, A study on species relationship in the section Brachypetalum of Paphiopedilum using RAPD technique, Ph.D. Thesis, Kasetsart University, Bangkok.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.U., 1990, DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucl. Acids Res. 8: 6531-6535.
Zhang, F., Lv, Y., Dong, H. and Guo, S., 2010, Analysis of genetic stability through inter simple sequence repeats molecular markers in micro propagated plantlets of Anoectochilus formosanus Hayata, a medicinal plant, Biol. Pharm. Bull. 33: 384-388.