Genetic Relationship among Paphiopedilum Subgenus Brachypetalum Section Brachypetalum Using HAT-RAPD Markers

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Teepaka Meesangiem
Somchit Damrianant
Theerachai Thanananta
Narumol Thanananta

Abstract

Abstract


Genetic relationship of lady’s slipper orchids, Paphiopedilum subgenus Brachypetalum section Brachypetalum, 14 varieties found in Thailand, was demonstrated by HAT-RAPD markers. The total of 72 random primers were screened and 29 primers could be used for DNA amplification. Twenty-two primers were selected and used to analyze each DNA species of all samples. A dendrogram was constructed, based on polymorphic bands, and showed genetic similarities among 4 clusters with the similarity coefficients ranging from 0.32 to 0.81 as well as the analysis of genetic diversity.


Keywords: Paphiopedilum; genetic relationship; HAT-RAPD marker

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How to Cite
Meesangiem, T., Damrianant, S., Thanananta, T., & Thanananta, N. (2017). Genetic Relationship among Paphiopedilum Subgenus Brachypetalum Section Brachypetalum Using HAT-RAPD Markers. Thai Journal of Science and Technology, 7(1), 99–105. https://doi.org/10.14456/tjst.2018.2
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วิทยาศาสตร์ชีวภาพ
Author Biographies

Teepaka Meesangiem

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Khlong Luang, Pathum Thani 12120

Somchit Damrianant

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Khlong Luang, Pathum Thani 12120

Theerachai Thanananta

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Khlong Luang, Pathum Thani 12120

Narumol Thanananta

Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under Royal Patronage, Khlong Nueng, Khlong Luang, Pathum Thani 13180

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